Method and process for extracting 4-androstenedione from phytosterol aqueous phase fermentation liquor

Abstract

The invention provides a method and process for extracting 4-androstenedione from phytosterol aqueous phase fermentation liquor. The method comprises the following steps: performing microbial fermentation on phytosterol serving as a raw material to generate 4-androstenedione; performing liquid-solid separation on the fermentation liquor generated at the end of fermentation through a centrifugal machine, and extracting a wet solid material; adding a acetone-purified water mixed solvent into the wet solid material, continuously stirring for 60-90 minutes under the condition that the temperature is raised to 40-60 DEG C, and performing solid-liquid separation under the temperature condition of higher than 40 DEG C; crystallizing the solution obtained through solid-liquid separation; centrifuging the crystallized material to obtain a coarse product of the 4-androstenedione, and refining the coarse product to prepare the finished 4-androstenedione product. According to the method, the impurities in the 4-androstenedione product can be reduced, and the extracting and refining process is simplified.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to a method and process for extracting 4-androstenedione from phytosterol aqueous phase fermentation liquid. Background technique [0002] 4-Androstenedione is an important intermediate in the synthesis of steroid hormone drugs. At present, it is mainly produced from phytosterols as the main raw material through microbial fermentation. Since it is difficult to convert all phytosterols into 4-androstenedione during the fermentation process, there are two substances of phytosterols and 4-androstenedione in the fermentation broth after the fermentation is finished. After the fermentation is finished, it is necessary to separate and extract 4-androstenedione from the fermentation broth with an appropriate organic solvent. [0003] At present, there are many kinds of organic solvents used in this extraction process in China, such as ethyl acetate, butyl acetate, acetone, methanol, ethanol, d...

AI Technical Summary

Problems solved by technology

The above-mentioned solvents have no selectivity (or low selectivity) to the two substances of phytosterol and 4-androstenedione. As a result, after the extraction of the above-mentioned solvents, both substances are extracted into the organic solvent, It has brought a lot of trouble to the subsequent separation and refining process (the purpose of refining is to prepare high-purity 4-androstenedione)

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