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Promoter of soybean flowering gene GmFT2a

A promoter and gene technology, applied in the field of soybean flowering gene GmFT2a promoter sequence, can solve problems such as affecting the growth period of soybean

Inactive Publication Date: 2014-05-21
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, how the promoter of the GmFT2a gene affects the soybean growth period (flowering period and maturity period) is a very important scientific issue, and there are few reports on related studies

Method used

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  • Promoter of soybean flowering gene GmFT2a
  • Promoter of soybean flowering gene GmFT2a
  • Promoter of soybean flowering gene GmFT2a

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Experimental program
Comparison scheme
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specific Embodiment approach 1

[0016] Embodiment 1: The nucleotide sequence of the promoter of the soybean flowering gene GmFT2a in this embodiment is shown in Seq ID No: 2 in the sequence table.

[0017] This embodiment is the first successful cloning and verification of the function of the promoter of the soybean flowering gene GmFT2a at the molecular level.

[0018] This embodiment is based on the cloning of the soybean flowering gene GmFT2a (Kong et al. 2010), and further systematic research on the promoter sequence of the GmFT2a gene. We have confirmed that the GmFT2a gene has the function of promoting soybean flowering. The promoter sequence of GmFT2a gene in different varieties was studied, and it was determined that the promoter of GmFT2a was closely related to soybean flowering period.

[0019] Through QTL technology analysis, it can be seen that the promoter of the flowering gene GmFT2a obtained from the soybean variety TK780 has the nucleotide sequence of Seq ID No: 1 in the sequence listing, and...

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Abstract

The invention provides a promoter of a soybean flowering gene GmFT2a, relates to a promoter sequence of the soybean flowering gene GmFT2a, and aims at providing the promoter of the soybean flowering gene GmFT2a. The nucleotide sequence of the promoter of the soybean flowering gene GmFT2a is as shown in Seq ID No: 2. The promoter of the GmFT2a plays a role in flowering promotion. Through soybean genetic transformation verification, the promoter of the GmFT2a plays a role in adjustment and control of flowering. The promoter of the soybean flowering gene GmFT2a has very important significance for disclosing molecular mechanisms in a soybean flowering period and a growth period; developed molecular markers can be used for molecular marker assistant breeding, so that the breeding process is accelerated, and the breeding efficiency is improved. The promoter of the soybean flowering gene GmFT2a is applied to the field of soybean genetic engineering.

Description

technical field [0001] The invention relates to the promoter sequence of soybean flowering gene GmFT2a, which belongs to the fields of plant genetic engineering and crop molecular breeding. Background technique [0002] Soybeans provide human beings with important vegetable proteins and oils. Worldwide, soybeans are cultivated in a wide range of regions ranging from high latitudes in northern Europe, Sweden and North America to Canada, and south to Brazil and Argentina. It is closely related to soybean photoperiod and growth period genes or quantitative trait loci (Quantitative Trait Locus, QTL). Breeding high-quality soybean varieties with wide ecological adaptability is the fundamental way to realize high-yield, high-quality and high-efficiency soybean industry. However, conventional breeding has made slow progress in the selection of widely-adapted varieties. The use of molecular breeding methods is expected to regulate the photoperiod and growth period of soybean in a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113
Inventor 孔凡江刘宝辉李莹曹东
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S