LC-QTOF (Liquid Chromatography-Quadrupole Time Of Flight) analysis method for distinguishing resveratrol of different resources

Abstract

The invention belongs to the field of analytic chemistry, resveratrol from polygonum cuspidatum and resveratrol from grape vine are taken as research objects, a liquid chromatography-mass spectrometry technology (LC-QTOF) with high sensitivity and high resolution is adopted for providing more characteristics of compound structural information, the difference of micro components in resveratrol samples is researched, four marking compounds which can distinguish resveratrol of different sources are found, the difference of the micro components in the resveratrol from polygonum cuspidatum and resveratrol from grape vine is further confirmed, a reference method is provided for distinguishing resveratrol samples of different raw material sources, and great significance for identifying the real property of the natural healthcare food, namely, resveratrol, is achieved, and meanwhile the technical method can be also widely popularized and applied to real property detection and identification standard of imported and exported agriculture products and biologic raw materials, technical demonstration for identification on the real property of the natural product raw material is provided, and establishment of reference standards is achieved.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and provides an LC-QTOF (liquid chromatography / quadrupole-time-of-flight mass spectrometry) analysis method for identifying the source of resveratrol raw materials. Background technique [0002] Resveratrol has a wide range of biological activities such as anti-tumor, prevention of cardiovascular diseases, immune regulation and antibacterial, and its special health benefits make it popular in the market. As the biological activity of resveratrol is gradually recognized, more and more health care product manufacturers are very optimistic about the functionality of resveratrol, and its application as a terminal product is expected to continue to expand. At this stage, the demand for resveratrol in domestic and foreign markets is increasing, and resveratrol will be more widely and more safely applied to various fields. [0003] Usually the sources of resveratrol are chemical synthesis, microorgan...

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Problems solved by technology

If high-purity Polygonum cuspidatum resveratrol is mixed with grape resveratrol, this method cannot identify the real source of resveratrol, and this method does not use other raw materials such as resveratrol from Polygonum cuspidatum However, it is not clear how to identify resveratrol from different sources of raw materials, and there are certain defects in the identification technology of resveratrol.

[0033] To a certain extent, this method provides a reference for identifying resveratrol products derived from grape vines, but it does not form a complete system for identifying the true properties of resveratrol from different sources, and this method cannot clearly identify how to identify different raw materials source of resveratrol, there are certain defects in the identification technology of resveratrol

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