Lonicera japonica detection primer, detection method and detection kit

Abstract

The invention discloses a loop-mediated isothermal amplification technology-based Chinese medicinal material lonicera japonica authenticity rapid-detection technology. A lonicera japonica detection primer can be used for amplifying the specific base of a target nucleic acid sequence, wherein the target nucleic acid sequence is a chloroplast sequence trnL-trnF. The primer is complementary with a part or a complementary chain of the No.582 to No.661 locus nucleic acid sequence of the target nucleic acid sequence. A primer group specific to a specific locus of lonicera japonica is provided, and a kit containing the primer group is used for detecting whether the specific locus of the lonicera japonica exists in the sample or not to further judge whether authentic lonicera japonica exists in the sample or not.

Description

Technical field [0001] The invention relates to a method for rapidly detecting honeysuckle using a loop-mediated isothermal amplification technology, and belongs to the technical field of molecular biology. Background technique [0002] According to market research and relevant experts, it can be used as fakes of Chinese herbal medicine honeysuckle, including Loniceramacranthoides Hand.-Mazz., Lonicera hypoglauca Miq., South China Lonicera (LoniceraConfusa DC.), or Yellow-brown Lonicera. (Lonicera fulvotomentosa HsuetS.C.Cheng), etc. (Hao Jinda, Practical Chinese herbal medicine experience identification, People's Medical Publishing House, 2009). The main methods for identifying medicinal materials included in the Pharmacopoeia of the People’s Republic of China (2010 Edition) include trait identification, thin-layer identification, etc. However, honeysuckle and its counterfeit Lonicera japonica, South China Lonicera, red glandular Lonicera, yellow-brown Lonicera, etc. The charac...

AI Technical Summary

Problems solved by technology

In the Pharmacopoeia of the People's Republic of China (2010 edition), the identification methods of medicinal materials mainly included character identification, thin-layer identification, etc. The characteristics are very similar, so the identification of traits is subjective to a certain extent, and the identifyr is required to have rich identification experience; if the shape of the medicinal material is incomplete or broken, it is also very difficult to identify

On the other hand, since the counterfeit products such as gray felted honeysuckle, southern China honeysuckle, red glandular honeysuckle, and yellow-brown honeysuckle all contain chlorogenic acid, so it is impossible to distinguish honeysuckle from its counterfeit products by TLC

[0003] The molecular biology-based detection methods developed in recent years, such as DNA barcodes and site-specific PCR, have overcome the shortcomings of traditional identification methods such as low accuracy and high subjectivity, and have realized the identification of honeysuckle medicinal materials under laboratory conditions. Accurate detection, but because conventional PCR requires expensive PCR machines or sequencers, gel electrophoresis and imaging systems, etc., the popularization and application of these detection methods are greatly limited

At present, there is no report on the detection of honeysuckle in honeysuckle, a traditional Chinese medicinal material, using LAMP technology

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