Composition, kit and application of composition and kit, as well as ammonia acid analysis method by using composition and kit

Abstract

The invention provides a composition which comprises a mixture with alcohol, acid, water and ninhydrin. The invention further provides a kit which comprises a container capable of containing one, two or three of the alcohol, the acid and the water and a container capable of containing the ninhydrin. The invention further provides application of the composition and the kit in analysis of ammonia acid and method for analyzing the ammonia acid. The method can be executed by the composition or the kit provided by the invention. The composition and the kit, provided by the invention, are high in developing property and high in resolution during analysis of the ammonia acid, particularly the ammonia acid in fermenting liquid, and can analyze at least 13 ammonia acids. The method is low in cost, easy to operate and high in detection speed of 10-25 minutes; furthermore, the method can analyze at least 13 ammonia acids under high resolution.

Description

technical field [0001] The invention relates to a composition and a kit, which are used for analyzing amino acids, especially amino acids in fermentation broth, and also relate to a method for analyzing amino acids, especially amino acids in fermentation broth. Background technique [0002] At present, the following methods are mainly used to analyze the amino acid components in fermentation broth: 1) high performance liquid chromatography or amino acid analyzer, although these two methods can perform quantitative analysis, but the instruments used are expensive and difficult to popularize; 2) paper chromatography Although this method does not require high equipment, it is relatively convenient, but this method takes a long time for each chromatography, usually 2-3 hours, and the total analysis time is as long as 4 hours, and the traditional paper chromatography method uses spray Color development, easy to develop uneven color or disperse stains, affecting analysis and judgm...

AI Technical Summary

Problems solved by technology

[0002] At present, the following methods are mainly used to analyze the amino acid components in fermentation broth: 1) high performance liquid chromatography or amino acid analyzer, although these two methods can perform quantitative analysis, but the instruments used are expensive and difficult to popularize; 2) paper chromatography Although this method does not require high equipment, it is relatively convenient, but this method takes a long time for each chromatography, usually 2-3 hours, and the total analysis time is as long as 4 hours, and the traditional paper chromatography method uses spray Color development, easy to develop uneven color or disperse stains, affecting analysis and judgment

[0003] Zhao Jianguo et al. in the document "Research on Protein and Amino Acids in Separation of Schizophrenic Protein by Thin Layer Chromatography", Journal of Hunan University of Traditional Chinese Medicine, April 2005, Volume 25, No. 2: Pages 26-29 disclose a Thin-layer chromatographic separation and identification method for the protein and amino acid components of the deadly cicada. Although this method can realize the analysis of the amino acids in the dead cicada protein, it takes 90 minutes for the development time, the total analysis time is longer, and the types of amino acids that can be analyzed are limited.