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Method for providing images of a tissue section

A technique for tissue sectioning and imaging, applied in the field of immunohistochemistry and computer-based image analysis

Active Publication Date: 2014-05-28
MEDETECT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Because the techniques described above were primarily developed for co-localization studies, they do not address the fact that many signature markers may also occasionally be present on unanticipated cell types

Method used

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  • Method for providing images of a tissue section
  • Method for providing images of a tissue section
  • Method for providing images of a tissue section

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0237] Example 1: Tissue processing, sample preparation and section generation.

[0238] Includes the following human tissues:

[0239] -Human terminal colon: Surgical resection due to inflammation and suspected indeterminate colitis.

[0240] -Human lung tissue from patients with chronic obstructive pulmonary disease, COPD and cystic fibrosis: due to lung resection due to suspected lung cancer, the analyzed tissue was unaffected by the cancer and was obtained as far away from the tumor as possible.

[0241] - Human Lymph Nodes: Larger draining lymph nodes collected in association with lung transplantation due to severe COPD or cystic fibrosis.

[0242] -Human tonsils: collected as part of a common tonsillectomy due to recurrent episodes of tonsillitis.

[0243] Samples (ie, tissue blocks) of all tissue types were subjected to common fixative immersion by immersion in common fixative (4% buffered formaldehyde pH 7.6). After fixing overnight, samples were dehydrated using ...

example 2

[0244] Example 2: Multiple immunohistochemical staining and generation of a series of digital images.

[0245] Immunohistochemical staining was performed using an Automated Immunohistochemical Automated Instrument (Autostainer CL-classic; Dako Cytomation, Glostrup, Denmark) with the DAKO REAL EnVision Detection System, a sensitive standard method intended for the detection of primary murine or rabbit antibodies (IHC kit Code K5007, Dako Cytomation, Denmark; see www.dako.com for more details). Primary antibodies for the detection of cell-specific antigens (formerly called "cell markers") are listed in List 2 and recommended by commercial manufacturers for immunohistochemistry on human tissues prepared for general pathology Stained dilutions were applied to sections (ie, sections from formalin-fixed and paraffin-embedded samples). An example of a panel of markers used in evaluating ESMS is listed in Table 3.

[0246] Table 2. Examples of antibodies used to experimentally val...

example 3

[0268] Example 3: Computerized image analysis and resolution of marker-specific staining patterns code.

[0269] The digitized slides corresponding to the series of master images from an Aperio slide scanner according to the present invention were manually inspected using viewing software (Aperio ImageScope, version 10.0.35.1798, Aperio Technologies, Inc.). During the initial evaluation, regions of interest in each slice were selected for further detailed analysis. Raw images (ie, master digital images) were exported as TIFF or JPEG files using the Extract and Export Image function in ImageScope software; one image for each staining cycle. Together these images form a series of images for each region of interest.

[0270] For some images, the distribution pattern of brown DAB deposits was drawn prior to image output after the color segmentation feature ("positive pixel" algorithm) included in the ImageScope software selected the RGG and Hue values ​​that characterize brow...

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Abstract

A method for differentiating areas in a series of digital images, the method comprising the steps of: providing a series of images comprising undetermined marker areas; evaluating every image I for 1<=n<=N according to predetermined selection criteria and defining image marker areas as undetermined marker areas fulfilling the predetermined selection criteria; providing a new image I; and inserting new image marker areas in the new image I, said new image marker areas having the same shape and location as image marker areas present in image I but not in image I, and said new image marker areas being identifiable in lnew by a unique feature. Further, the application discloses a method for visualizing cell populations in tissue sections of a histological sample. Further, the application discloses a method for visualizing three-dimensional distribution of multiple cell populations in a histological sample.

Description

technical field [0001] This application relates to the fields of immunohistochemistry and computer-based image analysis. More specifically, the present application provides methods for distinguishing regions in a series of images. Background technique [0002] Histological analysis of tissue samples is often used for diagnostic purposes, for example, analyzing breast tissue samples for the diagnosis of breast cancer, or for research purposes, for example, to study conditions such as asthma, atherosclerosis, or inflammatory bowel disease Inflammatory cell types under inflammatory conditions. [0003] Immunohistochemistry (IHC) to detect markers (ie, antigens) by antigen-specific antibodies is commonly used to identify cells in tissue sections. Ideally, cell type identification can be obtained by detecting cellular antigens specific to a cell. However, for multiple cell types, combinations of multiple antigens must be analyzed for proper recognition. [0004] Any diseased ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T7/00G06T9/00G06K9/62G06T11/00
CPCG06K9/00147G06T9/00G06T11/00G06F19/00G01N33/5005G06F19/10G06T7/0012G01N1/30G01N21/4795G01N21/77G01N21/78G01N2021/7786G01N2201/1247G06V20/695G06V20/698
Inventor 乔纳斯·埃延弗特
Owner MEDETECT
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