Use of nerve growth factor in preparing medicine for treating middle-aged and elderly male hypogonadism syndrome

A nerve growth factor, middle-aged and elderly technology, applied in the field of biomedicine

Active Publication Date: 2016-04-20
GUANGZHOU BIOPHARMACEUTICAL R&D CENT OF JINAN UNIV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The predecessors have also used cobra venom nerve growth factor to treat male reproductive defects and reduce the effect of gossypol on male spermatogenic function (ZL00116192.X), but whether nerve growth factor can be used to treat delayed hypogonadism syndrome has not yet been established. There are reports

Method used

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  • Use of nerve growth factor in preparing medicine for treating middle-aged and elderly male hypogonadism syndrome
  • Use of nerve growth factor in preparing medicine for treating middle-aged and elderly male hypogonadism syndrome
  • Use of nerve growth factor in preparing medicine for treating middle-aged and elderly male hypogonadism syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Detection of the pro-proliferation effect of rat nerve growth factor on rat Leydig cells

[0036] Materials: Coming 12-well plate (purchased from Corning, USA, product number: 3336), testosterone radioimmunoassay kit (purchased from Beijing North Institute of Biotechnology, product number: B10TFB), human nerve growth factor (purchased from Sigma-Aldrich Shanghai Trading Co., Ltd., product number: N1408-.1MG), rat nerve growth factor (purchased from Sigma-Aldrich Shanghai Trading Co., Ltd., product number: N2513-1MG) or mouse nerve growth factor (purchased from Sigma-Aldrich Shanghai Trading Co., Ltd. Ltd., product number: SRP4304-20UG), insulin-transferrin-sodium selenite medium supplement (ITS, purchased from Sigma-Aldrich Shanghai Trading Co., Ltd., product number: I1884), luteinizing hormone (LH, purchased from From Sigma-Aldrich Shanghai Trading Co., Ltd., item number: L9773), ethanedimethanylsulfone (EDS, synthesized by the School of Chemical Engineering...

Embodiment 2

[0039] Example 2: Detection of the differentiation-promoting effect of rat nerve growth factor on rat testicular mesenchymal stem cells

[0040] Male SPRAGUE-DAWLEY rats (purchased from Guangdong Provincial Medical Animal Experiment Center, 12 weeks old, 250 ± 20 g / rat) were intraperitoneally injected with ethanedimethylsulfone (EDS, 90 mg / kg body weight) 7 days before the experiment, and carbon dioxide After execution, the testes were taken out, placed in ice-cold phosphate buffer, the capsule was cut off, the blood vessels and seminiferous tubules were stripped, and the seminiferous tubules were separated into single ones, and placed in DMEM / F-12 medium (containing 0.1% Bovine serum albumin and 1x insulin-transferrin-sodium selenite medium supplement) were cultured overnight at 34°C and 5% carbon dioxide. The next day, the seminiferous tubules were divided into 24-well plates, and rat nerve growth factor was added for treatment. The concentration of rat nerve growth factor w...

Embodiment 3

[0042] Embodiment 3: Radioimmunoassay analysis of testosterone content in culture medium

[0043]Take 90-day-old SAMR1 mice (3 months old, body weight 26±2g), use forceps to extract the seminiferous tubules in the testes under sterile conditions, wash them with phosphate buffer for 6 times, and then culture them with DMEM / F-12 The base was washed 3 times, and the final concentration of 375 micrograms / milliliter (ug / ml) was added to the DMEM / F-12 medium containing 0.1% bovine serum albumin, and 24 Hour. After washing 3 times, change the fresh medium, then place it in an environment of 34°C and 5% carbon dioxide to cultivate, and change it every 3.5 days to contain different concentrations (10, 100 and 200 ng / ml) of human nerve growth factor, Cell culture medium for luteinizing hormone (LH) and platelet-derived growth factor AA (PDGFAA). Finally, the testosterone content in the medium was analyzed by radioimmunoassay. The experimental results showed that on day 14, nerve grow...

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PUM

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Abstract

Provided in the present invention is the use of a nerve growth factor in preparing a drug for treating hypogonadism syndrome in middle-aged and elderly males, which is mainly used to prepare a drug for treating decreasing human testosterone levels due to the reduction of leuteinizing hormones secreted by the pituitary gland or Leydig cell ageing, wherein the nerve growth factor is derived from human beings, mice or rats, preferably from human beings. The amino acid sequence of the nerve growth factor is shown as SEQ ID No:1-3.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to the second medical application of nerve growth factor. Specifically, the present invention relates to the use of human nerve cell growth factor in the preparation of medicines for treating hypogonadism in middle-aged and elderly males. Nerve cell growth factor derived from humans, rats and mice can be administered through nasal administration or testicular intravenous injection to treat human middle-aged and elderly males with delayed hypogonadism syndrome. Background technique [0002] Late onset hypogonadism in humans (late onset hypogonadismales, LOH) is a type of age-related testicular hypofunction in middle-aged and elderly men, and its main features include decreased libido and erection quality (especially nocturnal erection), mood changes accompanied by Decreased mental power and spatial orientation, decreased lean body mass (LBM) with decreased muscle volume and muscle ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61P15/00A61P15/10
CPCA61K38/185A61K38/1858A61K38/24A61P15/00A61P15/08A61P15/10A61K2300/00
Inventor 黄亚东苏志坚葛仁山陈洁项琪苏泽轩张齐好张磊
Owner GUANGZHOU BIOPHARMACEUTICAL R&D CENT OF JINAN UNIV CO LTD
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