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AvrBs3/pthA family gene jval containing HD to NG virulence factor modes

A technology of factors and patterns, applied in the field of avrBs3/pthA family gene jva1, can solve the problem of insufficient understanding of avrBs3/pthA family member genes

Active Publication Date: 2014-06-25
海宁市钱江兴业投资开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The invention provides an avrBs3 / pthA family gene jva1 containing HD to NG toxicity factor pattern, which is an avrBs3 / pthA family member gene in the Xoo Japanese race JXOV strain, and solves the current problem of the avrBs3 / pthA family in the Japanese race JXOV Insufficient understanding of member genes, especially in the middle region of jva1 which contains 12.5 repeat units and contains HD to NG virulence factor repeat patterns

Method used

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  • AvrBs3/pthA family gene jval containing HD to NG virulence factor modes

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Embodiment Construction

[0015] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0016] The first part, the gene cloning of jva1 of the present invention

[0017] The present invention contains the avrBs3 / pthA family gene jva1 of HD to NG toxicity factor pattern, and the ribonucleic acid molecular sequence of the gene is:

[0018]ATGGATCCCATTCGTTCGCGCACGCCAAGTCCTGCCCGCGAGCTTCTGCCCGGACCCCAACCGGATAGGGTTCAGCCGACTGCAGATCGGGGGGGGGCTCCGCCTGCTGGCGGCCCCCTGGATGGCTTGCCCGCTCGGCGGACGATGTCCCGGACCCGGCTGCCATCTCCCCCTGCGCCCTCGCCTGCGTTCTCGGCGGGCAGCTTCAGCGATCTGCTCCGTCAGTTCGATCCGTCGCTTCTTGATACATCGCTTCTTGATTCGATGCCTGCCGTCGGCACGCCGCATACAGCGGCTGCCCCAGCAGAATGGGATGAGGCGCAATCGGGTCTGCGTGCAGCCGATGACCCGCCACCCACCGTGCCTGTCGCTGTCACTGCCGCGCGGCCGCCGCGCGCCAAGCCGGCCCCGCGACGGCGTGCGGCGCAACCCTCCGACGCTTCGCCGGCCGCGCAGGTGGATCTACGCACGCTCGGCTACAGTCAGCAGCAGCAAGAGAAGATCAAACCGAAGGTGCGTTCGACAGTGGCGCAGCACCACGAGGCACTGGTGGGCCATGGGTTTACACACGCGCACATCGTTGCGCTCAGCCAACACCCGGCAG...

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Abstract

The invention discloses an avrBs3 / pthA family gene jval containing HD to NG virulence factor modes. The avrBs3 / pthA family gene jval is one avrBs3 / pthA family member gene in a Xoo Japanese microspecies JXOV bacterial strain, solves a problem that avrBs3 / pthA family member genes in the Japanese microspecies JXOV are not known enough, and particularly contains 12.5 repetitive units in a jva1 intermediate region and contains HD to NG virulence factor repetitive modes. The avrBs3 / pthA family gene jval has the beneficial effects that the jva1 gene has HD to NG eight virulence factor repetitive modes, is beneficial to promoting research on a pathogen toxicity mechanism, provides a new idea for preventing rice from bacterial blight, and also provides foundation for finding corresponding anti-disease gene in rice and breeding for disease resistance.

Description

technical field [0001] The invention relates to the technical fields of gene cloning and gene expression in biotechnology, in particular to an avrBs3 / pthA family gene jva1 containing HD to NG toxicity factor patterns. Background technique [0002] Rice (Oryza sativa) is an important food crop worldwide [1] , is also a model plant for cereal biology [2,3] . Bacterial blight of rice is one of the three major diseases of rice. Its pathogen is Xanthomonas oryza epv.oryzae (Xoo). There are many different races of Xoo, and more than 30 races of Xoo have been reported. [4] . [0003] Like other Xanthomonas species, Xoo has a Ш-type exudation system (T3S) necessary for pathogenicity, and T3S crosses the bacterial double membrane and probably directly penetrates the plant cell wall [5,6] . Bacterial effector proteins are secreted by T3S and enter plant cells [7] , these effector molecules act on different plant physiological processes, inhibit plant defense responses, regulate ...

Claims

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Application Information

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IPC IPC(8): C12N15/31
Inventor 张燕夏更寿
Owner 海宁市钱江兴业投资开发有限公司
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