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High performance liquid chromatography (HPLC) method for simultaneously determining 16 ginseng saponin monomers

A technology for ginsenosides and monomers, which is applied in the field of determination of ginsenoside monomer content, and can solve problems such as the simultaneous determination of ginsenoside monomer content, etc.

Inactive Publication Date: 2014-06-25
JILIN AGRICULTURAL UNIV
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Problems solved by technology

[0004] Although a large number of studies have reported HPLC assay methods for the content of ginsenoside monomers, most of them are methods for simultaneously measuring less than 12 kinds of ginsenoside monomers, and there is no report on the simultaneous determination of 16 kinds of ginsenoside monomers.

Method used

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  • High performance liquid chromatography (HPLC) method for simultaneously determining 16 ginseng saponin monomers
  • High performance liquid chromatography (HPLC) method for simultaneously determining 16 ginseng saponin monomers
  • High performance liquid chromatography (HPLC) method for simultaneously determining 16 ginseng saponin monomers

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Embodiment 1

[0018] Embodiment 1 A kind of simultaneous determination 16 kinds of ginsenoside monomer HPLC methods

[0019] 1. Materials

[0020] Japan Shimadzu LC-2010A high performance liquid chromatography, equipped with LC-2010A liquid chromatography pump, LC-2010A automatic sampler, CLASS-vP chromatography workstation; Japan Shimadzu AUY220 electronic analytical balance, Kunshan Shumei KQ -250DV ultrasonic cleaner.

[0021] Ginseng medicinal materials were purchased from Fusong County, Jilin Province and identified as ginseng by Professor Zhang Lianxue; ginseng stems and leaves were collected from the Medicine Plant Garden of Jilin Agricultural University; company, lot number Z22024479).

[0022] Ginsenoside reference substance Rg1(201022), Re(201035), Rf(201007), Rb1(201058), Rg2(201023), Rc(201069), Rb2(201078), Rb3(201029), F1(201225), Rd (201018), F2(201255), Rg3(201237), protopanaxatriol(201206), Compound K(201243), Rh2(2012009), protopanaxadiol(201235) were purchased from the...

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Abstract

The invention provides a high-performance liquid chromatography (HPLC) method for simultaneously determining 16 ginseng saponin monomers, such as Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, Rb3, F1, Rd, F2, Rg3, protopanaxatriol, Compound K, Rh2 and protopanaxadiol. The determination range comprises medicinal plants such as ginseng and American ginseng containing more than one saponin monomer, and preparations thereof. Compared with the prior art, the HPLC method has the advantages that the variety of the ginseng saponin monomers simultaneously determined by the method can be up to 16, and the efficiency is improved. Only are acetonitrile and water used as mobile phases, so that the problems that phosphate is large in loss on a chromatographic column, the system is troublesome to clean and the like are solved. The built HPLC method is wide in linearity range, accurate in method, good in reproducibility, fast and convenient, stable and reliable. A theoretical basis is provided for quality control of the medicinal plants, such as ginseng and American ginseng, and the preparations thereof.

Description

technical field [0001] The invention relates to a method for measuring the content of ginsenoside monomers in medicinal plants such as ginseng and American ginseng and preparations thereof, and belongs to the technical field of quality control of Chinese medicinal materials and preparations thereof. Background technique [0002] Ginseng is the most widely used representative of authentic medicinal materials in my country. Ginsenoside is the main medicinal component of ginseng. Ginsenoside has the functions of enhancing memory, improving immunity, improving cardiovascular, regulating endocrine, delaying aging and anti-tumor. Content determination has become a research hotspot at home and abroad. [0003] With the development of society, the requirements for drug quality control are getting higher and higher. However, due to the complex chemical composition of ginseng and the variety of ginsenosides, there are more than 50 kinds of ginsenoside compound monomers that have been i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 郜玉钢郭冲张连学臧埔何忠梅赵岩祝洪燕王立岩杨鹤刘双利
Owner JILIN AGRICULTURAL UNIV
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