A kind of multifunctional rice three-line male sterile line breeding method
A technology of three-line sterile line and multifunctional rice, which is applied in the field of breeding of multifunctional rice three-line sterile line, can solve the problem of low water-soluble protein content, and achieve good nutritional and health effects, agronomic traits and yield traits Excellent, gut-health-enhancing effects
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Embodiment 1
[0022] 1) Select high resistant starch content functional rice variety "Jiangtangdao No. 1" (A), low water-soluble protein rice variety "LGC21" (B), high-cellulose rice variety "Shuiyuan 464" (C), rich The special rice "Zibao Xiangnuo 1" (D) containing natural vitamin A was used for hybridization A / B and C / D to obtain hybrid F 1 Substituted by F 1-AB and F 1-CD express;
[0023] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / F 1-CD , have to resubmit F 1 , with F 1-ABCD express;
[0024] 3) Plant F 1-ABCD , F 2-ABCD , planting F 2-ABCD , a group of 2000 plants, harvest seeds from a single plant at maturity, measure the content of resistant starch, water-soluble protein, cellulose, and vitamin A, and screen to retain the content of resistant starch, cellulose, and vitamin A that are high in water-soluble low protein F 2-ABCD Single seed (F 3-ABCD ) 20 copies;
[0025] 4) Strain planting 3) F obtained by screening 3-ABCD , Investigate each l...
Embodiment 2
[0034] 1) Select high resistant starch content functional rice variety "Jiangtangdao No. 1" (A), low water-soluble protein rice variety "LGC21" (B), high-cellulose rice variety "Shuiyuan 464" (C), rich The special rice "Zibao Xiangnuo 1" (D) containing natural vitamin A was used for hybridization A / B and C / D to obtain hybrid F 1 Substituted by F 1-AB and F 1-CD express;
[0035] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / F 1-CD , have to resubmit F 1 , with F 1-ABCD express;
[0036] 3) Plant F 1-ABCD , F 2-ABCD , planting F 2-ABCD , a group of 2000 plants, harvest seeds from a single plant at maturity, measure the content of resistant starch, water-soluble protein, cellulose, and vitamin A, and screen to retain the content of resistant starch, cellulose, and vitamin A that are high in water-soluble low protein F 2-ABCD Single seed (F 3-ABCD ) 20 copies;
[0037] 4) Strain planting 3) F obtained by screening 3-ABCD , Investigate each l...
Embodiment 3
[0046] 1) Select high resistant starch content functional rice variety "Jiangtangdao No. 1" (A), low water-soluble protein rice variety "LGC21" (B), high-cellulose rice variety "Shuiyuan 464" (C), rich The special rice "Zibao Xiangnuo 1" (D) containing natural vitamin A was used for hybridization A / B and C / D to obtain hybrid F 1 Substituted by F 1-AB and F 1-CD express;
[0047] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / F 1-CD , have to resubmit F 1 , with F 1-ABCD express;
[0048] 3) Plant F 1-ABCD , F 2-ABCD , planting F 2-ABCD , a group of 2000 plants, harvest seeds from a single plant at maturity, measure the content of resistant starch, water-soluble protein, cellulose, and vitamin A, and screen to retain the content of resistant starch, cellulose, and vitamin A that are high in water-soluble low protein F 2-ABCD Single seed (F 3-ABCD ) 20 copies;
[0049] 4) Strain planting 3) F obtained by screening 3-ABCD In the flowering sta...
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