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Application of panax notoginseng flos saponin in preparing medicine for promoting angiogenesis

A technology for angiogenesis and total saponins, which is applied in the field of medicine, can solve the problems of less flowers of Panax notoginseng and underutilized medicinal value.

Active Publication Date: 2014-07-02
LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sanqi flower is commonly used in folks as a health care and treatment drug for hypertension and related diseases. Although it has a long history, there are relatively few studies on Sanqi flower at home and abroad, and its medicinal value has not been fully developed and utilized.

Method used

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  • Application of panax notoginseng flos saponin in preparing medicine for promoting angiogenesis
  • Application of panax notoginseng flos saponin in preparing medicine for promoting angiogenesis
  • Application of panax notoginseng flos saponin in preparing medicine for promoting angiogenesis

Examples

Experimental program
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Effect test

Embodiment 1 10

[0018] Example 1 Effect test of Panax notoginseng saponins on promoting angiogenesis in zebrafish model

[0019] 1. Materials and Methods

[0020] The animal transgenic zebrafish Tg(flila-EGFP)y1 was provided by Zebrafish International Resource Center (Oregon, PO), USA. Zebrafish were reared according to the zebrafish handbook method.

[0021] Drugs, reagents and instruments Panax notoginseng were provided by Yunnan Wenshan Science and Technology Innovation Center Co., Ltd. (origin: Wenshan, Yunnan). AB-8 macroporous adsorption resin (chemical plant of Nankai University, Tianjin) was selected, and the diameter-to-height ratio of the column was 1:10. Load 1.25g dry resin per gram of crude drug, and the sample concentration is 0.2g crude drug / ml. After fully adsorbed, wash with water until there is no Molish reaction, and then elute with 70% ethanol solution that is 3 times the volume of the resin. The adsorption and elution flow rate Both were 2 times the volume of the resin ...

Embodiment 2 10

[0031] Example 2 Effect experiment of Panax notoginseng saponins on the proliferation of human umbilical vein endothelial cells

[0032] Human umbilical vein endothelial cell culture: HUVECs were cultured in F-12K medium containing 10% inactivated newborn bovine serum, 1% penicillin-streptomycin, 100μg / ml heparin, 30μg / ml ECGS, 3-8 passages in logarithmic growth phase Cells are used for experiments, culture flasks for experiments, 24-well plates and 96-well plates are pre-coated with 0.1% gelatin;

[0033] Proliferation experiment of human umbilical vein endothelial cells: Seed human umbilical vein endothelial cells (HUVECs) in a 96-well plate at a density of 1×104 cells / well, culture for 24 hours, and replace with low-serum medium (0.5% FBS) The original culture medium (10% FBS), continue to culture for 24 hours to make the cells in a static state, and then replace the low Serum culture solution (culture solution containing 0.1% DMSO as blank control, 20ng / ml VEGF low serum ...

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Abstract

The invention belongs to the field of preparing traditional Chinese medicines, and relates to application of panax notoginseng flos saponin, as an active part extracted from Chinese herbal notoginseng flos, in preparing a medicine for promoting angiogenesis. Experimental results show that the panax notoginseng flos saponin disclosed by the invention has a protective effect on VRI induced vascular injury in an early stage and has a recovery effect of promoting angiogenesis in a later vascular development process; the panax notoginseng flos saponin has a proliferation function on human umbilical vein endothelial cells; moreover, the panax notoginseng flos saponin is more significant in effects of promoting angiogenesis and protecting blood vessel compared with panax notoginseng (root) saponin. The panax notoginseng flos saponin disclosed by the invention can serve as a raw material to further prepare a pharmaceutical preparation for promoting angiogenesis, and the pharmaceutical preparation is used for preventing and treating such diseases related to angiogenesis deficiency as coronary heart disease, myocardial ischemia, insufficient construction of collateral circulation complementarity, slow trauma and fracture healing and thronboangitis obliterans.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to the new application of total saponins of notoginseng flower in pharmacy, in particular to the application of total saponin of notoginseng flower in the preparation of drugs for promoting angiogenesis. More specifically, the present invention relates to the use of Panax notoginseng saponins in the preparation of drugs for treating diseases related to insufficient angiogenesis Background technique [0002] Coronary heart disease, myocardial ischemia, insufficient compensation of collateral circulation, slow healing of trauma and fractures, and obliterative vasculitis are all common clinical diseases and frequently-occurring diseases, which are important factors affecting the health and quality of life of the people. Insufficiency of angiogenesis is an important common pathophysiological basis of these diseases. Therefore, research on the effect of promoting angiogenesis is of great ...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P9/00A61P9/10A61P17/02A61P19/08A61P9/14A61K133/00
Inventor 王佑华李铭源周端许贝文魏莉
Owner LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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