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Extraction and detection method of aflatoxin b1 in fermented grains

A technology of aflatoxin and detection method, applied in the field of extraction and detection of aflatoxin B1 in fermented grains

Active Publication Date: 2016-10-05
LUZHOU PINCHUANG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection of aflatoxin B1 mainly focuses on grain and food, and distiller's grains in the liquor brewing process are a relatively complicated substance, and the existing detection methods cannot fully adapt to the detection of aflatoxin B1 in distiller's grains. Therefore, it is of great significance to establish a set of toxin detection methods that are accurate, low in cost, easy to operate, fast and adaptable to the liquor production process, and applied to the detection of aflatoxin B1 in the liquor brewing process.

Method used

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  • Extraction and detection method of aflatoxin b1 in fermented grains
  • Extraction and detection method of aflatoxin b1 in fermented grains
  • Extraction and detection method of aflatoxin b1 in fermented grains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Take a certain amount of comprehensive sample of unstrained spirits, put it in a sampling bag, take a certain amount of unstrained spirits, dry and pulverize, pass through a 20-mesh sieve, weigh 10g into a 250ml Erlenmeyer flask, add 45% (V / V) methanol- Extract the aqueous solution at a temperature of 20°C and an ultrasonic power of 140W for 15 minutes, filter, take 10ml of the filtrate into a 250ml Erlenmeyer flask, add 40ml of dichloromethane, shake for 5 minutes, pour it into a separatory funnel, let it stand for 25 minutes, and release the lower layer phase, repeat the extraction once, dry the dichloromethane layer in a 65°C water bath, and finally add 50% (V / V) methanol solution to be tested, use the kit and microplate reader to detect the sample to be tested, and the measured result is 2.0 μg / kg.

Embodiment 2

[0084] Take a certain amount of unstrained spirits, dry and pulverize, pass through a 20-mesh sieve, weigh 10g into a 250ml conical flask, add 85% (V / V) methanol-water solution, extract at a temperature of 20°C and an ultrasonic power of 200W for 40min , filter, take 10ml of the filtrate in a 250ml Erlenmeyer flask, add 40ml of dichloromethane, shake for 5min, pour it into a separatory funnel, let it stand for 25min, release the lower phase, repeat the extraction once, put the dichloromethane layer in a 65°C water bath Dry it, and finally add 50% (V / V) methanol solution to be tested. Use the kit and microplate reader to detect the sample to be tested, and the measurement result is 2.3 μg / kg.

Embodiment 3

[0086] Take a certain amount of unstrained spirits, dry and pulverize, pass through a 20-mesh sieve, weigh 10g into a 250ml conical flask, add 65% (V / V) methanol-water solution, extract at a temperature of 20°C and an ultrasonic power of 180W for 30min , filter, take 10ml of the filtrate in a 250ml Erlenmeyer flask, add 40ml of dichloromethane, shake for 5min, pour it into a separatory funnel, let it stand for 25min, release the lower phase, repeat the extraction once, put the dichloromethane layer in a 65°C water bath Dry it, and finally add 50% (V / V) methanol solution to be tested. Use a microplate reader to detect the sample to be tested, and the measurement result is 2.9 μg / kg.

[0087] Comparative example 1 national standard method to extract aflatoxin B1

[0088] Take a certain amount of unstrained spirits, dry and pulverize, sieve, weigh 20g of the sample in a 250ml Erlenmeyer flask, add 6ml of water dropwise with a dropper to wet the sample, add 60ml of chloroform accu...

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Abstract

The invention relates to a method or extracting aflatoxin B1 in fermented grains, belonging to the field of analysis and detection. The method for extracting aflatoxin B1 in fermented grains provided by the invention comprises the following steps: dried fermented grains are weighed, screened, undergo ultrasonic wave concussion by adding a methanol aqueous solution, dichloromethane or acetone and then are filtered, the filtrate is taken for later use and extracted by using dichloromethane, ethyl acetate or acetone, and then the dichloromethane, ethyl acetate or acetone layer is collected and dried. With the method, aflatoxin B1 can be quickly and accurately extracted from fermented grains, and then is detected by using enzyme linked immunosorbent assay; the method for extracting and detecting aflatoxin B1 in fermented grains provided by the invention can be applied to extraction and detection of aflatoxin B1 in fermented grains, and provides a better approach for research of aflatoxin B1.

Description

technical field [0001] The invention belongs to the field of analysis and detection, and in particular relates to a method for extracting and detecting aflatoxin B1 in fermented grains. Background technique [0002] Aflatoxin (Aflatoxin, AFT) is mainly a class of toxic secondary metabolites produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. It is widely found in crops and food. The hazard of aflatoxin lies in the damage to human and animal livers. The tissue has a destructive effect, which can lead to liver cancer or even death in severe cases. [0003] Liquor brewing mainly uses grain as raw material, so there is a possibility of contamination by aflatoxin B1, so it is particularly important to do a good job in the detection of aflatoxin B1 during the brewing process of liquor. At present, the detection of aflatoxin B1 mainly focuses on grain and food, and distiller's grains in the liquor brewing process are a relatively complicated substance, and th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/14G01N33/53
CPCC07D493/14G01N33/56961
Inventor 胡竹行沈才洪敖宗华王松涛王明廖源周军
Owner LUZHOU PINCHUANG TECH CO LTD
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