Preparation method of color chart for rapidly detecting content of methemoglobin

A methemoglobin and colorimetric card technology, which is applied in the field of rapid detection of methemoglobin content colorimetric card preparation, can solve the problems of high equipment requirements, poor clinical effect, slow detection speed, etc., and achieve the effect of easy use

Inactive Publication Date: 2014-07-09
CHONGQING MEDICAL UNIVERSITY
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Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method for rapid detection of methemoglobin blood content colorimetric card, aiming to solve the problems of high equipment requirements, slow detection speed and poor clinical effect of existing detection methods

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  • Preparation method of color chart for rapidly detecting content of methemoglobin
  • Preparation method of color chart for rapidly detecting content of methemoglobin
  • Preparation method of color chart for rapidly detecting content of methemoglobin

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Embodiment Construction

[0022] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0023] The present invention provides a kind of preparation method of rapid detection methemoglobin blood content color comparison card, such as figure 1 shown, including the following steps:

[0024] S1. Use different concentrations of nitrite solutions to act on multiple first-class blood samples from different tissue sources, and draw the effect-dosage relationship diagram between nitrite and methemoglobin production rate;

[0025] In step S1, more specifically, the following steps are included:

[0026] A. Collect venous blood samples from 50 healthy people, define the venous blood samples as...

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Abstract

The invention provides a preparation method of a color chart for rapidly detecting content of methemoglobin. The method comprises the following steps: by respectively taking nitrite solutions with different concentrations as blood samples, drawing an effect-dose relational graph between nitrite and methemoglobin production rate; preparing the blood samples with different methemoglobin concentrations according to the effect-dose relational graph; and respectively dripping the blood samples with different methemoglobin concentrations on a blank color chart for scanning and measuring a red, green and blue (RGB) value, performing statistical analysis, and drawing a color chart of methemoglobin of different concentrations, and finally printing the color chart for use. The color chart prepared by the preparation method can rapidly detect the content of methemoglobin, is simple and convenient to use and is safe and economic.

Description

technical field [0001] The invention belongs to the technical field of methemoglobinemia detection, in particular to a preparation method of a colorimetric card for rapid detection of methemoglobin content. Background technique [0002] Methemoglobin (MetHb) is produced by red blood cells either genetically or after absorption of toxic compounds. When its content exceeds 1% of the total hemoglobin, it can cause methemoglobinemia (methemoglobinemia), which can be divided into two categories: acquired and hereditary according to the cause. Acquired methemoglobinemia is the most common, mostly due to ingestion of certain oxidative (reductive) drugs (such as sulfonamides, chloroquine, primaquine, etc.) or poisons (such as nitrite, ammonium nitrate, potassium nitrate, etc. ) transient performance. Hereditary methemoglobinemia (HM) is mostly caused by the genetic defect of NADH-cytochrome b5 reductase (NADH-cytochrome b5 reductase, b5R), which is an autosomal recessive inheritan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/72
Inventor 李剑波王徐
Owner CHONGQING MEDICAL UNIVERSITY
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