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Method for obtaining a transformed ipomoea batatas plant body including highly accumulated carotenoid and anthocyanin, and plant body obtained by same

A technology of carotene and phytosomes, which is applied to the preparation of transformed sweet potato phytosomes that accumulate a large amount of carotenoids and anthocyanins and the field of phytosomes prepared therefrom, which can solve the problems of inability to meet the requirements of commercialization and low productivity , to achieve the effect of high use value and enlarged harvest

Inactive Publication Date: 2014-07-09
KOREA RES INST OF BIOSCI & BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Many studies have been conducted to produce useful substances from transformed plants, but the productivity is too low to meet the requirements of commercialization

Method used

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  • Method for obtaining a transformed ipomoea batatas plant body including highly accumulated carotenoid and anthocyanin, and plant body obtained by same
  • Method for obtaining a transformed ipomoea batatas plant body including highly accumulated carotenoid and anthocyanin, and plant body obtained by same
  • Method for obtaining a transformed ipomoea batatas plant body including highly accumulated carotenoid and anthocyanin, and plant body obtained by same

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preparation example Construction

[0042] Moreover, the present invention provides a method for preparing transformed sweet potato plants that accumulate a large amount of anthocyanins with increased tolerance to environmental stress compared to the wild type, which includes adding IbOr-Ins containing the base sequence of SEQ ID NO.1 The recombinant vector of the gene mutant is transformed into the sweet potato plant that accumulates anthocyanin, thereby making the step of overexpressing the IbOr-Ins gene mutant, and the IbOr-Ins gene mutant is artificially inserted into the IbOrange gene derived from sweet potato derived from a specific base sequence.

[0043] According to the method of an embodiment of the present invention, the sweet potato plant body accumulating anthocyanins is preferably purple sweet potato, more preferably sweet potato of Xinzimei variety, but not limited thereto.

[0044] According to the method of an embodiment of the present invention, the aforementioned environmental stress is prefer...

Embodiment 1

[0049] Cloning and base sequence analysis of embodiment 1.IbOr-Ins gene

[0050] PCR primers for cloning sweet potato Orange gene were prepared. The primer sequences are as follows: forward primer (5'-atggtatattcaggtagaatcttgtcgctc-3'; SEQ ID No.2) and reverse primer (5'-ttaatcaaatgggtcaattcgtgggtcatg-3'; SEQ ID No.3). Using the thus obtained IbOr as a template, overlapping PCR was performed to artificially mutate the specific base sequence of IbOr. In order to insert the nucleotide sequence deduced to be -KSQNPNL- from the 133rd amino acid in the amino acid sequence of the IbOrange gene (see Korean Patent No. 0990330), PCR primers were prepared, and the primer sequences used at this time were as follows: Forward Primer (5'-gaaaagcaagaaaataaacttaa atcccagaaccctaac-3'; SEQ ID No. 4) and reverse primer (5'-aagatttgcggatgtcaggtt agggttctgggattaag-3'; SEQ ID No. 5). As a result, a PCR product of about 921 bp was obtained, cloned into the pGEM-T-vector, and sequenced to confirm t...

Embodiment 2

[0052] Embodiment 2. Transformation is derived from the IbOr-Ins gene variant of sweet potato in new Zimei sweet potato somatic cell culture cell

[0053] Under the regulation of the 35S promoter, the IbOr-Ins gene mutant derived from sweet potato was transformed into a large number of anthocyanin-containing sweet potato cultivar Xinzimei somatic cell culture cells ( figure 2 A). As a result, transformed plantlets were obtained from the selection medium, and using this as an object, the expression of the transformant selection marker hygromycin gene HPT II was measured by genomic DNA PCR to select transformants. The above transformants were labeled Zm-Or ( figure 2 B).

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Abstract

The present invention relates to a method for obtaining a transformed Ipomoea batatas plant body having high accumulations of carotenoid and anthocyanin by transforming a recombinant vector, which includes an IbOr-Ins gene mutation obtained by artificially inserting a specific base sequence into an IbOrange gene derived from Ipomoea batatas, into an Ipomoea batatas plant body accumulating anthocyanin. The present invention further relates to a transformed Ipomoea batatas plant body including highly accumulated carotenoid and anthocyanin obtained by the method, and to seeds thereof. The present invention further relates to a method for obtaining a transformed Ipomoea batatas plant body including highly accumulated anthocyanin and having increased resistance to environmental stress compared to a wild plant body by transforming the recombinant vector into an Ipomoea batatas plant body accumulating anthocyanin, to a transformed Ipomoea batatas plant body including highly accumulated anthocyanin obtained by the method and having increased resistance to environmental stress compared to a wild plant body, and to seeds thereof. The present invention further relates to an amount of carotenoid in the Ipomoea batatas plant body accumulating anthocyanin and including the IbOr-Ins gene mutation, and to a composition for increasing resistance to environmental stress.

Description

technical field [0001] The present invention relates to a method for preparing transformed sweet potato plants that accumulate carotenoids and anthocyanins in large quantities and the plants prepared therefrom; A preparation method for transforming sweet potato plants, which is transformed into a large amount of accumulating anthocyanins by artificially inserting the recombinant vector of the IbOr-Ins gene mutant with a specific base sequence into the IbOrange gene derived from sweet potato (Ipomoea batatas) Realized in the sweet potato plant body of element; A large amount of accumulated carotenoids and anthocyanin transformed sweet potato plant and its seeds prepared by the above method; a kind of transformed sweet potato plant that accumulates anthocyanin with improved environmental stress tolerance compared with wild type A preparation method, which is obtained by transforming the above-mentioned recombinant vector into a large amount of sweet potato plants accumulating an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/00C12N15/29C12N15/82
CPCC07K14/415C12N15/825C12N15/8271C12N15/8205C12N15/8218C12N15/8273
Inventor 郭尚洙李幸顺金善荷朴成喆郑在哲
Owner KOREA RES INST OF BIOSCI & BIOTECH
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