Method for transferring bean rhizobium to root systems of sunflowers for biological nitrogen fixation

Abstract

The invention relates to a cultivation technology in the field of crops, in particular to a new method for transferring rhizobium of beans to the root systems of sunflowers for biological nitrogen fixation through an ultra-distant combined breeding technology. According to the method, through sexual propagation (hybridization) of the sunflowers and the beans, nitrogenase of the beans is transferred to the sunflowers so that the root systems of the sunflowers can nodulate, but cross pollination cannot be performed on the sunflowers and the beans with the ultra-distant relationship; asexual propagation (grafting) must be performed first so that the genetic relationship between the sunflowers and the beans can be closer, and it is possible to achieve sexual propagation; the combined breeding technology is characterized in that success of grating of the sunflowers and the beans must be guaranteed first, particularly, grafting should be performed when seedlings just come up but do not send forth true leaves, only the seedlings at the time can interchange with one another most actively, and the variation probability is the largest. But, the seedlings just coming up are extremely weak, the plants are small and tender, and the grafting difficulty is very high.

Description

technical field [0001] The invention relates to a cultivation technology in the field of crops, in particular to a new method for transferring soybean rhizobia to sunflower roots to carry out biological nitrogen fixation by using super-distant compound breeding technology, that is, through sexual reproduction of sunflower and soybean, soybean Nitrogenase is transferred to sunflower, so that nodules can form on the root system of sunflower, but super-distant relationship between sunflower and soybean cannot be cross-pollinated. In order to make sunflower and soybean hybrid successfully, sunflower and soybean must first be asexually propagated , because asexual reproduction can increase the affinity between the two, and on the basis of improving the affinity of the two, the sexual reproduction can be successfully hybridized. The key to this compound breeding technology is to first ensure the successful grafting of sunflower and soybean. Background technique [0002] Biological...

AI Technical Summary

Problems solved by technology

At present, there is still a patented technology in China called "method for rhizobia transfer by remote grafting" with the application number 201010046310.9 (publication (announcement) date: 2010.06.23). The idea of ​​using grafting to transfer rhizobia is correct. But there are two shortcomings: one, it is too late for grafting when rootstock and scion grow to 1-6 leaves, although the plant is strong and strong at this time, it is easy to graft and survive, but at this time the plant attributes have been basically qualitative, and the active period has passed. Difficult to change each other's traits

Second, this technology only has grafting and no hybridization. Originally, the purpose of grafting is to create conditions for hybridization, because only hybridization can breed new varieties, and grafting cannot breed new varieties. Grafting is only a means rather than an end.

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