Microfluidic nucleic acid extraction device and method based on fta card

Abstract

The invention relates to a microfluidic nucleic acid extraction device and method based on a FTA (fault tree analysis) card, and the device comprises a sample introduction unit, an extracting unit, a collection unit and a power device; the sample introduction unit includes multiple reagent containers and a first control valve, wherein the multiple reagent containers are respectively loaded with a variety of sample introduction components for making a nucleic acid extraction liquid, the first control valve connects the multiple reagent containers and can switch the reagent containers to allow the reagent containers to be connected to the extracting unit, the first control valve is connected with the power device, the power device provide power for liquid or gas flow among the sample introduction unit, the extracting unit and the collection unit, the extracting unit is internally provided with a containing space for embedding the FTA card, the collection unit includes an extraction liquid collecting container for collection of the nucleic acid extraction liquid; the whole device is simple and convenient to complete nucleic acid extraction operation, fast in extraction, and convenient for preservation and analysis of nucleic acids, can handle complex samples, and has the advantages of simple preparation and low cost.

Description

Technical field [0001] The invention relates to a nucleic acid extraction device, in particular to a nucleic acid extraction device and method based on FTA card microfluidics. Background technique [0002] At present, the rapid extraction and complete preservation of nucleic acids are in huge demand in the fields of scientific research and testing. Because the related operations can be simplified, solid phase extraction has attracted more and more attention. The solid-phase extraction technology of nucleic acid is a technology that does not require centrifugal operation, but only needs to use the principle that nucleic acid can be combined with or separated from the solid-phase matrix under different liquid environments to perform accounting extraction or preservation. Since there is no need for a centrifuge, the solid phase extraction device is usually very small and simple, and it is also convenient for automation or integration with other downstream technologies. At present,...

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Problems solved by technology

Using microfluidic chip technology, people have tried to integrate silicon bead method and magnetic bead method into the chip, but there are still various problems in these devices, for example, Chinese patent application numbers are 200880023815.4, 201110372801.7 and 201010042681.X Although the nucleic acid microfluidic extraction method mentioned in the literature can initially extract the required nucleic acid, there are various problems such as complicated steps, high cost, difficult operation, and limited scope of application.

At the same time, the research and development attempts of integrating FTA cards into microfluidic devices have just begun, such as integrating FTA immobilized cards as solid-phase extraction media and chips made of PMMA, but the disadvantage of this device is that the processing is very complicated and the cost is relatively high

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