Method for constructing electro-catalytic bacterial biofilm at anode of microbial electrochemical reactor

A technology of microbial electrochemistry and bacterial biofilm, applied in the direction of stress-stimulated microbial growth, biochemical equipment and methods, methods of supporting/immobilizing microorganisms, etc., can solve poor bearing capacity, low electrocatalytic efficiency, and limit operating current Small problems, to achieve the effect of improved catalytic activity, strong catalytic activity, and large catalytic current

Inactive Publication Date: 2014-07-30
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The electrocatalytic bacterial biofilm constructed by the potential adaptation method generally has the following shortcomings: (1) the limit working current that the electrode can reach is small, and the electrocatalytic efficiency is low; Poor endurance, when the working current passing through the electrode approaches or exceeds its limit current, the electrode will be rapidly over-polarized, and over-polarization will cause the anode potential to move positively to +1.2V in a short period of time (usually no more than 10 minut

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  • Method for constructing electro-catalytic bacterial biofilm at anode of microbial electrochemical reactor
  • Method for constructing electro-catalytic bacterial biofilm at anode of microbial electrochemical reactor
  • Method for constructing electro-catalytic bacterial biofilm at anode of microbial electrochemical reactor

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Embodiment 1

[0024] The present invention constructs the method for electrocatalytic bacterial biofilm at the anode of microbial electrochemical reactor, comprises the following steps successively:

[0025] (1) Assemble the microbial electrochemical reactor, which includes an anode chamber and a cathode chamber.

[0026] ⑵ if figure 1 The circuit components are connected as shown, and the circuit components include a selection switch QS1, a fixed resistor R0 with a resistance value of 500Ω, a DC stabilized power supply DV1 with an output voltage of 24V, an ammeter A, and a variable resistor whose resistance value is adjustable within the range of 0 to 40000 ohms. Resistor RX, auxiliary switch QS2 and cycle time relay KT with adjustable on / off time ratio in the range of (0.1s~10 s): (0.1s~10 s), select the common terminal of the switch QS1 to connect with the anode, select The first selection terminal of the switch QS1 is connected with the cathode in series with the fixed resistor R0, and...

Embodiment 2

[0036] The present invention constructs the method for electrocatalytic bacterial biofilm at the anode of microbial electrochemical reactor, comprises the following steps successively:

[0037] (1) Microbial electrochemical reactor is installed, and the microbial electrochemical reactor includes an anode chamber and a cathode chamber.

[0038] ⑵ if figure 1 The circuit components are connected as shown, and the circuit components include a selection switch QS1, a fixed resistor R0 with a resistance value of 500Ω, a DC stabilized power supply DV1 with an output voltage of 24V, an ammeter A, and a variable resistor whose resistance value is adjustable within the range of 0 to 40000 ohms. Resistor RX, auxiliary switch QS2 and cycle time relay KT with adjustable on / off time ratio in the range of (0.1s~10 s): (0.1s~10 s), select the common terminal of the switch QS1 to connect with the anode, select The first selection terminal of the switch QS1 is connected with the cathode in se...

Embodiment 3

[0048] The present invention constructs the method for electrocatalytic bacterial biofilm at the anode of microbial electrochemical reactor, comprises the following steps successively:

[0049] (1) Microbial electrochemical reactor is installed, and the microbial electrochemical reactor includes an anode chamber and a cathode chamber.

[0050] ⑵ if figure 1 The circuit components are connected as shown, and the circuit components include a selection switch QS1, a fixed resistor R0 with a resistance value of 500Ω, a DC stabilized power supply DV1 with an output voltage of 24V, an ammeter A, and a variable resistor whose resistance value is adjustable within the range of 0 to 40000 ohms. Resistor RX, auxiliary switch QS2 and cycle time relay KT with adjustable on / off time ratio in the range of (0.1s~10 s): (0.1s~10 s), select the common terminal of the switch QS1 to connect with the anode, select The first selection terminal of the switch QS1 is connected with the cathode in se...

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Abstract

The invention relates to a method for constructing an electro-catalytic bacterial biofilm at an anode of a microbial electrochemical reactor. The method comprises the following steps: firstly assembling the microbial electrochemical reactor and connecting with circuits, wherein the first path is formed by connecting a fixed resistor between the anode and a cathode in series, and the second path is formed by connecting a direct current stabilized power supply, an ampere meter, a variable resistor and a working contact of a cycle time relay between the anode and the cathode in series; then injecting a bacterial growth culture medium liquid into an anode chamber, inoculating a bacteria source, conducting the first path to carry out prestart on the microbial anode, measuring the anode potential change to obtain a polarization curve and determining an ultimate current value of the anode; and after electro-catalytic bacterial biofilm is stimulated by using ultimate pulse current to grow, successively obtaining a new ultimate current value again, then stimulating again under the new ultimate pulse current, repeating the steps till no significant growth of the ultimate current exists and finishing construction of the anodic electro-catalytic bacterial biofilm. The electro-catalytic bacterial biofilm can stably run under relatively high working current.

Description

technical field [0001] The invention relates to a method for constructing an electrocatalyzed bacterial biofilm on the anode of a microbial electrochemical reactor, and belongs to the technical field of microbial electrochemical reactors. Background technique [0002] Microbial electrochemical reactors include microbial fuel cells, microbial electrolysis cells, and microbial electrochemical sensors. In microbial electrochemical reactors, the electrocatalytic activity and operational stability of the electrocatalytic bacterial biofilm attached to the electrode surface are the key components that determine the performance of the reactor. A stable and efficient electrocatalytic bacterial biofilm can improve the performance of microbial fuel cells and microorganisms. Energy efficiency of electrolytic cells, increased sensitivity of microbial electrochemical sensor signal responses. Electrocatalytic bacterial biofilm is a cell accumulation layer with a certain spatial structure ...

Claims

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Application Information

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IPC IPC(8): C12M1/42H01M4/88
CPCC12M25/02C12M43/00
Inventor 张恩仁汪霄刘奕刁国旺
Owner YANGZHOU UNIV
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