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Method for batch induction of scophthalmus maximus tetraploid fries

A technology of turbot and tetraploid, applied in the field of chromosome manipulation

Inactive Publication Date: 2014-08-06
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the situation that the treatment time cannot be accurately determined in the previous tetraploid induction experiment and the induction efficiency is affected, the purpose of the present invention is to provide a method for batch induction of turbot tetraploid fry

Method used

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  • Method for batch induction of scophthalmus maximus tetraploid fries
  • Method for batch induction of scophthalmus maximus tetraploid fries
  • Method for batch induction of scophthalmus maximus tetraploid fries

Examples

Experimental program
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Effect test

Embodiment 1

[0026] In the fertilization period, incubation period and induction period of this embodiment, the seawater temperature used in the three periods is a constant water temperature suitable for fertilization and hatching of turbot eggs, that is, the seawater temperature in the three periods of the fertilization period, incubation period and induction period is 14.5 ± 0.1°C.

[0027] 1) Collection of gametes

[0028] Select broodstock that are in the process of laying eggs and expecting to lay them gently on the cushion, wipe off the water and dirt around their body surface and reproductive holes, and then slowly squeeze from back to front, and squeeze the sperm and eggs into the beaker respectively Or reserve in a basin. Observed under the dissecting microscope, the eggs are spherical, transparent, with concentrated oil globules, moderate in diameter and uniform in size;

[0029] 2) Artificial insemination

[0030] Control group artificial insemination:

[0031] Take 0.1mL of...

Embodiment 2

[0044] In the fertilization period, incubation period and induction period of this embodiment, the seawater temperature used in the three periods is a constant water temperature suitable for the fertilization and hatching of turbot eggs, that is, the seawater temperature in the three periods of the fertilization period, the incubation period and the induction period is 15.0 ± 0.1°C.

[0045] 1) Collection of gametes

[0046] Select broodstock that are in the process of laying eggs and expecting to lay them gently on the cushion, wipe off the water and dirt around their body surface and reproductive holes, and then slowly squeeze from back to front, and squeeze the sperm and eggs into the beaker respectively Or reserve in a basin. Observed under an anatomical microscope, the eggs are spherical, transparent, with concentrated oil globules, moderate in diameter and uniform in size;

[0047] 2) Artificial insemination

[0048] Control group artificial insemination:

[0049] Ta...

Embodiment 3

[0062] In the fertilization period, incubation period and induction period of this embodiment, the seawater temperature used in the three periods is a constant water temperature suitable for fertilization and hatching of turbot eggs, that is, the seawater temperature in the three periods of the fertilization period, incubation period and induction period is 15.5 ± 0.1°C.

[0063] 1) Collection of gametes

[0064] Select broodstock that are in the process of laying eggs and expecting to lay them gently on the cushion, wipe off the water and dirt around their body surface and reproductive holes, and then slowly squeeze from back to front, and squeeze the sperm and eggs into the beaker respectively Or reserve in a basin. Observed under an anatomical microscope, the eggs are spherical, transparent, with concentrated oil globules, moderate in diameter and uniform in size;

[0065] 2) Artificial insemination

[0066] Control group artificial insemination:

[0067] Take 0.15mL of...

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Abstract

The invention relates to a chromosome manipulation technique, in particular to a method for batch induction of scophthalmus maximus tetraploid fries. The method comprises the steps as follows: selecting pubescent scophthalmus maximus parent fishes, and respectively collecting seminal fluid and fish eggs for standby use; taking the seminal fluid and the fish eggs at the volume ratio of 1:(100-200) to perform fertilization, the process of which is used as a control group; after artificial fertilization is performed for 15-25 min in the control group, taking collected seminal fluid and fish eggs at a volume ratio of 1:(100-200) to perform fertilization, the process of which is used as an induction group; taking the time when 70-80 percent of fertilized eggs in the control group generate egg cracks for the first time as the time when fertilized eggs in the induction group generate egg cracks for the first time, and in the time period of 10-20 min before egg cracks are generated for the first time in the induction group, putting the fertilized eggs in the induction group into a pressurizing vessel of a hydrostatic press, performing pressure shock treatment for 6-8 min under the hydrostatic pressure of 60-70 MPa and performing pressure relief, taking out the fertilized eggs and putting into seawater for continuous culturing; measuring the tetraploid rate in the gastrula period and fry period. By adopting the method, the tetraploid rate can be up to 90 percent, and scophthalmus maximus tetraploid fries can be obtained efficiently and stably through induction.

Description

technical field [0001] The invention relates to chromosome manipulation technology, in particular to a method for inducing turbot tetraploid fry in batches by utilizing the principle of hydrostatic pressure to suppress fertilized egg cleavage. Background technique [0002] Turbot (Scophthalmus maximus) belongs to the Scophthalmidae family and the genus Scophthalmus. It grows fast and has become the main marine aquaculture species in northern my country. The aquaculture technology and industrial chain are relatively complete. However, in recent years, the turbot breeding industry has seriously degraded its germplasm due to generations of breeding and inbreeding. Therefore, it is urgent to adopt effective methods to genetically improve turbot and fundamentally solve the problem of improved species in turbot breeding industry. Polyploidy has always been concerned by researchers and breeders because of its short cycle, quick effect and high safety. Among them, triploid fish ha...

Claims

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Application Information

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IPC IPC(8): A01K61/00A01K61/10
CPCY02A40/81
Inventor 尤锋吴志昊胡金伟马得友谭训刚张培军
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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