Bacillus licheniformis, microbial agent and application of bacillus licheniformis or microbial agent in mine ecological remediation

A technology of bacillus licheniformis and microbial agent, which is applied in the field of ecological restoration of mines and bacillus licheniformis, can solve the problems of low development and difficulty in further maintaining the stability of mine slopes, and achieve the effect of increasing stability

Active Publication Date: 2014-08-06
王夕刚
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the process of regreening the slope, commonly used plants include shrubs such as Lespedeza bicolorTurcz. and / or Amorpha fruticosa Linn. The degree of development in the slope is not high, and it is difficult to further maintain the stability of the mine slope

Method used

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  • Bacillus licheniformis, microbial agent and application of bacillus licheniformis or microbial agent in mine ecological remediation

Examples

Experimental program
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Embodiment approach

[0023] Among them, as a particularly preferred embodiment of the present invention, the solid medium contains 400-700g / kg of straw, 100-300g / kg of bean cake powder, 2-10g / kg of potassium dihydrogen phosphate and 1-10g of / kg of ferrous sulfate. Wherein, the solid medium may also contain the rest of water and / or other components. In this preferred mode, on the one hand, Bacillus licheniformis can grow rapidly during the fermentation process, and on the other hand, the obtained fermented material can be used as a microbial agent, which can better colonize the slope soil and establish a microenvironment.

[0024] Wherein, the straw can be crushed into a conventional size that can be used for fermentation, for example, the length can be 5-10mm, the width can be 3-8mm, and the thickness can be 3-8mm. The straw may be straw from various sources, for example including at least one of corn straw, sorghum straw, rice straw, wheat straw and soybean straw.

[0025] Among them, the ferm...

Embodiment 1

[0031] This example is used to illustrate the cultivation of Bacillus licheniformis of the present invention and the preparation of microbial inoculum.

[0032] The preservation number is CCTCC NO: M2013497 Bacillus licheniformis (Bacillus licheniformis) was inoculated into LB medium (containing 10g / L of tryptone, 5g / L of yeast extract and 10g / L of NaCl), at 35 ℃ and 80 Cultivate under rev / min shaking until the concentration of live bacteria is 1010 CFU / mL to obtain seed bacteria liquid.

[0033] Sprinkle 100mL of the above-mentioned seed bacteria liquid evenly into 100kg of fermentation medium (the fermentation medium contains 600g / kg of straw (corn stalk, crushed to a size of about 10mm long, 5mm wide, and 5mm thick), 300g / kg of bean cake powder, 5g / kg of potassium dihydrogen phosphate, 5g / kg of ferrous sulfate and the rest of water), stir evenly and then ferment at 30°C. During the fermentation process, samples are taken and observed by microscope direct counting method , ...

Embodiment 2

[0035] This example is used to illustrate the cultivation of Bacillus licheniformis of the present invention and the preparation of microbial inoculum.

[0036] The preservation number is CCTCC NO: M2013497 Bacillus licheniformis (Bacillus licheniformis) was inoculated into LB medium (containing 10g / L of tryptone, 5g / L of yeast extract and 10g / L of NaCl), at 35 ℃ and 80 Cultivate under rev / min shaking until the concentration of live bacteria is 5×10 9 CFU / mL to obtain the seed bacterial liquid.

[0037]Sprinkle 100mL of the above-mentioned seed bacteria liquid evenly into 100kg of fermentation medium (the fermentation medium contains 500g / kg of straw (corn stalk, crushed to a size of about 10mm long, 5mm wide, and 5mm thick), 200g / kg of bean cake powder, 5g / kg of potassium dihydrogen phosphate, 5g / kg of ferrous sulfate and the rest of water), stir evenly and ferment at 30°C. During the fermentation process, samples are taken and observed by microscope direct counting method ...

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Abstract

The invention provides a bacillus licheniformis of which the preservation number is CCTCC NO: M2013497. The invention further provides a microbial agent. The microbial agent is prepared by the preparation method comprising the following steps: inoculating the bacillus licheniformis into a liquid culture medium for cultivation to obtain a seed liquid; (2) inoculating the seed liquid into a solid culture medium for fermentation to obtain fermented materials. The invention further provides application of the bacillus licheniformis or the microbial agent in mine ecological remediation. By the adoption of the technical scheme, the root growth of green plants can be remarkably promoted and reach to a higher flourishing degree, and the stability of side slopes is further increased.

Description

technical field [0001] The invention relates to the field of microorganism application, in particular to a Bacillus licheniformis, microbial agents containing the Bacillus licheniformis and their application in mine ecological restoration. Background technique [0002] Mine restoration refers to the restoration of the pollution of mining abandoned land to realize the reuse of land resources. During the mining process, a large amount of land that cannot be used without treatment will be produced, also known as mining abandoned land. There are various pollutions caused by production in the abandoned land. [0003] There are many reasons for mining abandoned land, including the accumulation of stripped topsoil, the accumulation of rock fragments and low-grade ore, the formation of empty mining areas and subsidence areas, the formation of tailings accumulation, and the unusable land affected by mining. In a broad sense, it also includes the accumulation of smelting slag formed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00E02D17/20C12R1/10
Inventor 王夕刚
Owner 王夕刚
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