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Portable pathogen detector

A detector and pathogen technology, applied in the field of medical detection, can solve the problems of large size, inconvenient carrying, high cost, etc., and achieve the effect of small size, good effect and convenient use

Inactive Publication Date: 2014-08-06
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above defects or improvement needs of the prior art, the present invention provides a pathogen detector, the purpose of which is to solve the problem of large volume, inconvenient portability and high cost of the current pathogen detector through compact design and selection of its various components. question

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0045] A pathogen detection instrument, such as figure 2 As shown, it includes a transparent amplification reaction chip 1 , a temperature controller, a heater 2 , a radiator 3 , a fluorescence excitation unit 4 and a fluorescence reception unit 5 .

[0046] The amplification reaction chip 1, such as image 3 As shown, it is made of glass, which is a microarray reaction chip, including a top sample introduction layer 11, a top reaction layer 12, a bottom reaction layer 13 and a bottom substrate layer 14 placed from top to bottom; wherein the top sample introduction layer 11 Bond with the top reaction layer 12 to form a top chip, and the bottom reaction layer 13 and the bottom substrate layer 14 are bonded to form a bottom chip; the sample injection holes 15, reaction holes 16, and sample outlet holes 17 are arranged on the top chip and the bottom chip. The communication holes and communication channels are arranged radially on the circumference of different radii with the ce...

Embodiment 2

[0054] A pathogen detection instrument, such as Figure 6 As shown, it includes a transparent amplification reaction chip 1 , a temperature controller 6 , a heater 2 , a heat sink 3 , a fluorescence excitation unit 4 and a fluorescence reception unit 5 .

[0055] The amplification reaction chip 1, such as Figure 7 As shown, it is made of plexiglass. The amplification reaction chip 1 can also include two layers placed from top to bottom, the upper layer 18 is a polydimethylsiloxane layer provided with a sample inlet 15, a pipeline and a sample outlet 17, and the lower layer is provided with a reaction hole 16 glass layer, the polydimethylsiloxane layer is covered on the glass layer, and each reaction hole 16 communicates with the corresponding sampling hole 15 and the sampling hole 17 through the channel on the covering layer. The reaction sample enters the reaction hole 16 on the glass layer through the injection hole 15 on the polydimethylsiloxane layer, is tested in the r...

Embodiment 3

[0063] A pathogen detection instrument, such as Figure 10 As shown, it includes a transparent amplification reaction chip 1 , a temperature controller 6 , a heater 2 , a heat sink 3 , a fluorescence excitation unit 4 and a fluorescence reception unit 5 .

[0064] The amplification reaction chip 1, such as Figure 11 Shown is Quartz. Described amplification reaction chip 1 is a kind of porous plate, comprises upper and lower two layers, and lower layer 19 is the transparent glass layer that is provided with reaction hole 16, and upper layer 18 is the covering layer that has connection channel, sample injection hole 15 and sample outlet hole 17 The glass layer is equipped with an array of reaction wells 16 in 2 rows and 4 columns, each reaction well 16 is independent, and each reaction well 16 communicates with the corresponding sampling hole 15 and sampling hole 17 on the covering layer.

[0065] The thermostat 6, such as Figure 12 As shown, it is a temperature sensor DS18...

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Abstract

The invention discloses a pathogen detector. The pathogen detector comprises a transparent amplified reaction chip, a temperature controller, a heater, a radiator, a fluorescence excitation unit and a fluorescence receiving unit, wherein the amplified reaction chip is provided with a reaction hole and is embedded on the heater; the temperature controller is arranged between the amplified reaction chip and the heater; the radiator is arranged below the heater; the fluorescence excitation unit is vertically arranged above the reaction hole of the amplified reaction chip; the fluorescence receiving unit is arranged above the reaction hole of the amplified reaction chip and forms an angle alpha together with the fluorescence excitation unit; the angle alpha ranges from 0 to 80 degrees. The pathogen detector is compact in structure, small in size, convenient to carry, low in cost, simultaneously good in constant temperature control effect and high in detection precision, and can be applied to bulk detection.

Description

technical field [0001] The invention belongs to the field of medical detection, and more specifically relates to a portable pathogen detection instrument. Background technique [0002] Nucleic acid amplification is one of the most commonly used methods in gene diagnosis technology. At present, there are many nucleic acid amplification methods, but they are rarely used in clinical diagnosis and On-site real-time detection occasions. The method has the advantages of simplicity, rapidity, strong specificity, high amplification efficiency and easy detection of products. [0003] The principle of fluorescence detection is that when microorganisms or compounds are irradiated by light of a certain wavelength, they can be excited to emit fluorescence, and the measurement of the emitted fluorescence energy is fluorescence detection. Different substances generally only absorb light of specific wavelengths and produce fluorescence of specific wavelengths, so fluorescence detection me...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 冯晓均刘笔锋刘志华夏赟
Owner HUAZHONG UNIV OF SCI & TECH
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