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Method for measuring chemical components in urine and excrement of rat orally taking relinqing granules

A technology of hot drenching and feces clearing, which is applied in the field of research on the metabolism of Chinese patent medicines, and can solve problems such as the lack of description of the content determination method

Active Publication Date: 2015-05-13
GUIZHOU WARMEN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the excretion research of protocatechuic acid, there are literatures (Huang Yong et al., UPLC-ESI-MS / MS method to study the excretion of Compound Orangis for injection in rats, Chinese patent medicine, 2012) on its content in urine The determination method has been studied, but its content determination method in feces is not described in the literature

Method used

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  • Method for measuring chemical components in urine and excrement of rat orally taking relinqing granules
  • Method for measuring chemical components in urine and excrement of rat orally taking relinqing granules
  • Method for measuring chemical components in urine and excrement of rat orally taking relinqing granules

Examples

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Embodiment 1

[0175] Method for determining the chemical composition in rat urine after oral administration of Relinqing Granules: Take 0.5 g of Relinqing Granules and dissolve them in 5 times of water to make an aqueous solution with a concentration of 0.1 g / mL, and administer them to rats by intragastric administration (245g) 2.5mL aqueous solution of hot shower clear granules, collect urine for 0-2h, centrifuge the urine in a low-speed centrifuge at 4000r / min for 10min, transfer the upper layer liquid to a new blank EP tube, press with ethyl acetate Extract at a volume ratio of 1:4 for 5 minutes, vortex evenly for 30 seconds, then centrifuge at a constant temperature at 4°C at a speed of 12,000r / min for 10 minutes, transfer the supernatant to a new EP tube, repeat 3 times, and blow at 40°C in a nitrogen blower. After drying, the residue was dissolved in 100 μL of mobile phase, vortexed for 60 seconds, then 12000 r / min, kept at 4°C, centrifuged for 10 minutes, and 10 μL of sample was taken...

Embodiment 2

[0182] Method for determining the chemical composition in rat urine after oral administration of Relinqing Granules: take 1 g of Relinqing Granules and dissolve them in 8 times of water to make an aqueous solution, and give rats (265 g) Relinqing Granules aqueous solution by intragastric administration 2.65mL, collect 10-12h of urine, centrifuge the urine in a low-speed centrifuge at 4000r / min for 10min, transfer the upper layer liquid to a new blank EP tube, extract with ethyl acetate at a volume ratio of 1:4 for 5min, Vortex evenly for 30s, then centrifuge at constant temperature at 4°C, rotate at 12000r / min, centrifuge for 10min, transfer the supernatant to a new EP tube, repeat 3 times, blow dry at 40°C with a nitrogen blower, and wash the residue with 100μL mobile phase Dissolve, vortex and mix for 60 seconds, then 12000r / min, 4°C constant temperature, centrifuge for 10 minutes, take 10μL sample, and treat the blank urine in the same way, using liquid chromatography-mass s...

Embodiment 3

[0189] Method for determining the chemical composition in rat urine after oral administration of Relinqing Granules: Take 4g of Relinqing Granules and dissolve them in 10 times of water to make an aqueous solution, and give rats (273g) Relinqing Granules aqueous solution by intragastric administration 2.73mL, collect 24-48h of urine, centrifuge the urine in a low-speed centrifuge at 4000r / min for 10min, transfer the upper layer liquid to a new blank EP tube, extract with ethyl acetate at a volume ratio of 1:4 for 5min, Vortex evenly for 30s, then centrifuge at constant temperature at 4°C, rotate at 12000r / min, centrifuge for 10min, transfer the supernatant to a new EP tube, repeat 3 times, blow dry at 40°C with a nitrogen blower, and wash the residue with 100μL mobile phase Dissolve, vortex and mix for 60 seconds, then 12000r / min, 4°C constant temperature, centrifuge for 10 minutes, take 10μL sample, and treat the blank urine in the same way, using liquid chromatography-mass sp...

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Abstract

The invention discloses a method for determining two components in urine and excrement of a rat orally taking relinqing granules and a method for measuring contents of the two components thereof. The content measuring method is used for measuring contents of gallic acid and protocatechuic acid in the urine and excrement of the rat orally taking relinqing granules by adopting high performance liquid chromatography-mass spectrum combined detection method on the basis of the established detecting method. The content measuring method disclosed by the invention is high in precision, good in repeatability, good in stability, accurate in measured result and capable of effectively evaluating excretion conditions of the gallic acid and protocatechuic acid in the relinqing granules in a living body.

Description

technical field [0001] The invention belongs to the technical field of in vivo metabolism research of Chinese patent medicines, and specifically relates to a method for determining two components in urine and feces of rats after oral administration of Relinqing granules and their content determination methods. Background technique [0002] Relinqing granule is a unilateral preparation made from the seedling medicine Polygonum chinensis, which is recorded in the 2010 edition of "Chinese Pharmacopoeia". It has the effects of clearing away heat and detoxification, diuresis and treating stranguria. It is used for damp-heat stagnation, yellow-red urine, dripping and astringent pain, urinary tract infection, and pyelonephritis with the above syndromes. It has definite curative effect on urinary system infection. "Relinqing Granule" is a national protected variety of traditional Chinese medicine, a national social security drug, and the only Miao medicine that has entered the 201...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/15G01N30/88
Inventor 周欣龚小见陈华国马风伟赵杨梁斌杨槐张丽艳
Owner GUIZHOU WARMEN PHARMA