Electropositive metal fluorescent nanodots, preparation method and application in cell fluorescence imaging
A positively charged metal, fluorescent nanotechnology, applied in the application of cell fluorescence imaging, surface positively charged metal fluorescent nanodots, in the field of preparation, can solve the problems of harsh conditions, complicated synthesis methods, etc., and achieve mild conditions, The effect of good optical properties and water solubility, and simple preparation method
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Embodiment 1
[0017] Preparation of electropositive Au nanodots. Add 0.1mmol AgNO to 100mL water 3 , after mixing evenly, add 0.2 mmol of sodium citrate, heat the solution to 100°C for 1 hour, and then cool it naturally to obtain a gray turbid solution. The reacted solution is directly centrifuged, and the precipitate is collected. The obtained Ag nanoparticles have a particle size of 60 ~ 80nm, dispersed in 100mL water.
[0018] Take the above 5mL AgNPs solution, add 20μL, 50mmol / LHAuCl 4 Solution, after mixing evenly, add 100μL, 0.005mmol / L polyethyleneimine as a stabilizer, and stir the system evenly in an oil bath at 70°C for 2h, you can see that the flask becomes an orange-red transparent solution with a small amount of black precipitate generate. After cooling, first centrifuge the reaction solution directly to separate the black precipitate, add 30mL isopropanol precipitant to the obtained supernatant, centrifuge, disperse the precipitate into 5mL water, repeat 3 times, and obtain...
Embodiment 2
[0020] Preparation of electropositive Pd nanodots. Add 0.2mmol CH in 150mL water 3 COOAg, after mixing evenly, add 0.3mmol sodium borohydride, heat the solution to 90°C and react for 50 minutes, then cool naturally to obtain a gray turbid solution, centrifuge the reacted solution directly, and take the precipitate, and the obtained Ag nanoparticles have a particle size of 60 ~80nm, dispersed in 100mL water.
[0021] Take the above 5mL AgNPs solution, add 30μL, 50mmol / LPdCl 2 Solution, after mixing evenly, add 200μL, 0.003mmol / L polyallylamine hydrochloride, and stir the system evenly in an oil bath at 80°C for 3h, you can see that the flask becomes a light yellow-green transparent solution with a small amount of black precipitate generate. After cooling, first centrifuge the reaction solution directly to separate the black precipitate, add 50mL isopropanol precipitant to the obtained supernatant, centrifuge, disperse the precipitate into 5mL water, repeat 3 times, and obtai...
Embodiment 3
[0023] Preparation of electropositive Pt nanodots. Add 0.15mmolAgNO to 90mL water 3 , after mixing evenly, add 0.3mmol ascorbic acid, heat the solution to 100°C for 30min, then cool naturally, centrifuge the reacted solution directly, take the precipitate, and obtain Ag nanoparticles with a particle size of 60-80nm, which are dispersed in 100mL water. Take the above 5mL AgNPs solution, add 50μL, 50mmol / LH 2 PtCl 6 Solution, after mixing evenly, add 100μL, 0.01mmol / L polyethyleneimine as a stabilizer, and stir the system uniformly in an oil bath at 80°C for 3h, you can see that the flask becomes a light yellow transparent solution with a small amount of black precipitate generate. After cooling, first centrifuge the reaction solution directly to separate the black precipitate, add 50mL isopropanol precipitant to the obtained supernatant, centrifuge, disperse the precipitate into 5mL water, repeat 3 times, and obtain a small amount of yellow solid precipitate, which is PEI st...
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