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Electropositive metal fluorescent nanodots, preparation method and application in cell fluorescence imaging

A positively charged metal, fluorescent nanotechnology, applied in the application of cell fluorescence imaging, surface positively charged metal fluorescent nanodots, in the field of preparation, can solve the problems of harsh conditions, complicated synthesis methods, etc., and achieve mild conditions, The effect of good optical properties and water solubility, and simple preparation method

Active Publication Date: 2015-11-18
深圳金昱纳米生物材料科技有限公司
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Problems solved by technology

It has been reported that the synthesis of electropositive nanomaterials is mainly a two-step method (Nanoscale, 2013, 5, 6154–6160). The synthesis method is cumbersome and the conditions are relatively harsh, and a variety of ligands need to be added during the reaction process.

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  • Electropositive metal fluorescent nanodots, preparation method and application in cell fluorescence imaging
  • Electropositive metal fluorescent nanodots, preparation method and application in cell fluorescence imaging
  • Electropositive metal fluorescent nanodots, preparation method and application in cell fluorescence imaging

Examples

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Effect test

Embodiment 1

[0017] Preparation of electropositive Au nanodots. Add 0.1mmol AgNO to 100mL water 3 , after mixing evenly, add 0.2 mmol of sodium citrate, heat the solution to 100°C for 1 hour, and then cool it naturally to obtain a gray turbid solution. The reacted solution is directly centrifuged, and the precipitate is collected. The obtained Ag nanoparticles have a particle size of 60 ~ 80nm, dispersed in 100mL water.

[0018] Take the above 5mL AgNPs solution, add 20μL, 50mmol / LHAuCl 4 Solution, after mixing evenly, add 100μL, 0.005mmol / L polyethyleneimine as a stabilizer, and stir the system evenly in an oil bath at 70°C for 2h, you can see that the flask becomes an orange-red transparent solution with a small amount of black precipitate generate. After cooling, first centrifuge the reaction solution directly to separate the black precipitate, add 30mL isopropanol precipitant to the obtained supernatant, centrifuge, disperse the precipitate into 5mL water, repeat 3 times, and obtain...

Embodiment 2

[0020] Preparation of electropositive Pd nanodots. Add 0.2mmol CH in 150mL water 3 COOAg, after mixing evenly, add 0.3mmol sodium borohydride, heat the solution to 90°C and react for 50 minutes, then cool naturally to obtain a gray turbid solution, centrifuge the reacted solution directly, and take the precipitate, and the obtained Ag nanoparticles have a particle size of 60 ~80nm, dispersed in 100mL water.

[0021] Take the above 5mL AgNPs solution, add 30μL, 50mmol / LPdCl 2 Solution, after mixing evenly, add 200μL, 0.003mmol / L polyallylamine hydrochloride, and stir the system evenly in an oil bath at 80°C for 3h, you can see that the flask becomes a light yellow-green transparent solution with a small amount of black precipitate generate. After cooling, first centrifuge the reaction solution directly to separate the black precipitate, add 50mL isopropanol precipitant to the obtained supernatant, centrifuge, disperse the precipitate into 5mL water, repeat 3 times, and obtai...

Embodiment 3

[0023] Preparation of electropositive Pt nanodots. Add 0.15mmolAgNO to 90mL water 3 , after mixing evenly, add 0.3mmol ascorbic acid, heat the solution to 100°C for 30min, then cool naturally, centrifuge the reacted solution directly, take the precipitate, and obtain Ag nanoparticles with a particle size of 60-80nm, which are dispersed in 100mL water. Take the above 5mL AgNPs solution, add 50μL, 50mmol / LH 2 PtCl 6 Solution, after mixing evenly, add 100μL, 0.01mmol / L polyethyleneimine as a stabilizer, and stir the system uniformly in an oil bath at 80°C for 3h, you can see that the flask becomes a light yellow transparent solution with a small amount of black precipitate generate. After cooling, first centrifuge the reaction solution directly to separate the black precipitate, add 50mL isopropanol precipitant to the obtained supernatant, centrifuge, disperse the precipitate into 5mL water, repeat 3 times, and obtain a small amount of yellow solid precipitate, which is PEI st...

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Abstract

The invention provides an electropositive fluorescent metal nanodot, a preparation method and application of the electropositive fluorescent metal nanodot in terms of cell fluorescence imaging, and belongs to the technical field of fluorescent nano materials. The method includes the steps that silver nanoparticles of large sizes are obtained by means of synthesis of reducing agents under the heating condition firstly and serve as template agents, a certain quantity of water-soluble electropositive polymers are added to the silver nanoparticles and serve as stabilizing agents, metal ions with certain concentration are added, the mixtures are evenly mixed and are heated and stirred for a period of time, and water-soluble metal nanodots are prepared; after centrifugation, the part of solutions is deposited through isopropyl alcohol, a solid is obtained through centrifugation and then is scattered through water, and finally the electropositive metal nanodots dispersed in aqueous solutions are finally obtained. The preparation method for the electropositive fluorescent metal nanodots is simple, moderate in condition, easy to operate, good in repeatability, capable of meeting the requirement of mass production and applied to the fields such as cell fluorescence imaging and marking, genetic loads, transfection and gene therapy.

Description

technical field [0001] The invention belongs to the technical field of fluorescent nanometer materials, and in particular relates to a positively charged metal fluorescent nanometer point on the surface, a preparation method and an application in cell fluorescence imaging. Background technique [0002] Compared with the previous small molecule dyes and fluorescent proteins, metal nanofluorescent materials have superior photophysical properties, large specific surface area, simple surface targeting and fluorescent tunability, making them useful in biosensing , Molecular imaging, optoelectronics, nanomedicine and other fields have broad application prospects and become the focus of scientists. At present, a variety of metal fluorescent nanomaterials have been reported to be synthesized, such as Cu (Small, 2013, 22, 3873–3879), Pd (Chem.Commun., 2011, 47, 5750-5752), Ag (Angew.Chem.Int .Ed.,2010,49,3925–3929), Au (J.Am.Chem.Soc.,2009,131,888–889), most of these materials show ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24B82Y40/00C09K11/58C09K11/87
Inventor 林权孙源卿陈阳陈洁杨旭东杨雪杨柏董凤霞
Owner 深圳金昱纳米生物材料科技有限公司
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