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Termitomyces albuminosus strain with high yield of cerebroside compounds and mycelium cultivation method for termitomyces albuminosus strain

A technology of cerebrosides and gallinaceous fungi, applied in the field of microorganisms, can solve the problems of low content of cerebrosides A and B and the like

Inactive Publication Date: 2014-09-03
金珠满江农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a high-yielding cerebroside compounds of the collybia fruticosa bacterial classification, overcome the problem that the content of cerebroside A and B in the fruit body of the wild collybia fruticosa is relatively low

Method used

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  • Termitomyces albuminosus strain with high yield of cerebroside compounds and mycelium cultivation method for termitomyces albuminosus strain
  • Termitomyces albuminosus strain with high yield of cerebroside compounds and mycelium cultivation method for termitomyces albuminosus strain
  • Termitomyces albuminosus strain with high yield of cerebroside compounds and mycelium cultivation method for termitomyces albuminosus strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] This example illustrates mycelium induction and strain purification.

[0059] 1. Collection of fruiting bodies of wild Gallus teratum

[0060] The fruiting bodies of Gallinarum albumen were collected from the mountainous area of ​​Jianshui County, Honghe Prefecture, Yunnan Province in August 2013, numbered, and stored in a refrigerator at 4-10°C.

[0061] 2. Hyphae induction

[0062] Clean and sterilize the collected fruiting body of the wild Gallus luteum, take the tissue of the fruiting body with a scalpel, and cut into about 0.1cm 3 of small pieces. The tissue pieces (86 pieces in total) were respectively connected to test tubes equipped with slant medium, placed in a constant temperature incubator at 25° C. for 5-16 days, and hyphae of each tissue were induced. The slant medium was prepared as follows. Recipe (g / L): Potato extract powder 15, glucose 20, agar 20, yeast extract powder 2, pH 5; sterilize at 121°C for 20 minutes; cool to about 60°C at room temperatu...

Embodiment 2

[0066] This example illustrates the screening of strains.

[0067] 1. Cell culture.

[0068] strain 51-S M -2 / P2, 51-S T -1 / P1 and 51-P-3 / P1 were used as objects for strain screening.

[0069] Try to select colonies with the same growth potential, punch holes at the edge of the plate colony with a sterile puncher with a pore diameter of 5 mm, take out circular colony slices with a diameter of 5 mm, and inoculate to a new plate medium (formula (g / L): potato Extract powder 15, glucose 20, agar 20, yeast extract powder 2, pH5). Each bacterial strain was connected with 6 plate mediums, and each plate medium was connected with 3 bacterial blocks. Cultivate at a constant temperature of 28°C, measure the diameter of the colony regularly, and observe the color, shape and growth of the colony.

[0070] figure 1 Indicates the change of colony diameter of each strain during plate culture. 27 days before plate culture, strain 51-S M -2 / P2, 51-S TThere was no significant differenc...

Embodiment 3

[0095] This example illustrates the identification of bacterial species.

[0096] 1. Morphological identification.

[0097] The conidia of the strain CTM-1 were oval, with a size of 5-10 μm×10-15 μm. The mycelium is less branched, with a diaphragm, no lock joints, and the diameter of mycelium is about 5-7μm; The characteristics of mycelium and conidia of this strain are consistent with those of Termitomyces albuminosus (Nie Xiaodong, optimization of submerged fermentation conditions for mycelia and saponins [D]. Jiangnan University, 2009). like Figure 5 shown.

[0098] 2. Molecular biological identification.

[0099] The 18SrRNA sequence of the fruiting body raw material (Fruiting body-5) used for mycelia induction is as follows:

[0100]

[0101] The 18S rRNA sequence of strain CTM-1 is as follows:

[0102]

[0103] Using the sequence local similarity query system BLAST (National Center for Biotechnology Information, USA), the 18S rRNA sequences of fruiting body ...

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Abstract

The invention relates to a termitomyces albuminosus strain Termitomyces sp. CTM-1 with the high yield of cerebroside compounds. The strain preservation number is CCTCC NO: M2014185. The invention further relates to a method for cultivating the termitomyces albuminosus strain. Mycelium dry matter with the yield of 15.90 g / L can be obtained through liquid cultivation of the termitomyces albuminosus strain, the content of cerebroside A and the content of cerebroside B in mycelia can reach 0.29 percent and 0.11 percent respectively, and the content of the cerebroside A and the content of the cerebroside B are higher than those in a wild termitomyces albuminosus strain. Artificial cultivation and industrial production of the termitomyces albuminosus strain can be achieved, and the problems that physical resources are rare, recovery seasonality is strong, the price is high, and the content of the cerebroside A and the content of the cerebroside B are low of the wild termitomyces albuminosus strain are solved. The strain preparation manner and the mycelium cultivation manner are also suitable for producing mycelia rich in cerebroside through liquid cultivation with other edible fungi as raw materials.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of Gallus arborvitae strain with high production of cerebroside compounds and a method for cultivating its mycelium. Background technique [0002] Pain is one of the most common clinical symptoms. Pain plagues people's health and quality of life, and long-term severe pain (such as cancer pain, intractable pain, etc.) is an unbearable torture. In the world, the prevalence of chronic pain is as high as 35.5%. There are at least 100 million pain patients in the United States and more than 200 million pain patients in China, but about 85% of the patients' pain has not been relieved in time. The existing problems of clinical analgesics include: 1. Narcotic analgesics and antipyretic analgesics can relieve the pain of patients, but there are still a large number of pains that cannot be effectively controlled; Neglected side effects—toxicity, addiction, and tolerance; 3 Analgesics...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 缪冶炼花卫俊陈玲袁丽红
Owner 金珠满江农业有限公司
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