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Method for producing dendrobine by inducing dendrobium candidum by salicylic acid

Inactive Publication Date: 2014-09-03
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Dendrobium candidum has low reproductive rate, slow growth, over-harvesting and ecological environment damage under natural conditions, which lead to the endangerment of wild resources and limit the production and further development and utilization of Dendrobium
With the maturity of tissue culture technology, scholars at home and abroad use tissue culture technology to rapidly reproduce Dendrobium officinale to improve the reproduction coefficient and solve the problem of resource shortage. The content, chemical composition and effect are equivalent to those of wild Dendrobium, which still cannot effectively solve the problem of shortage of Dendrobium resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Step 1. Preparation of Dendrobium candidum tissue culture seedlings: use the seeds of Dendrobium candidum from Yandang Mountain, Zhejiang, and sow them on the surface of 1 / 2MS medium with 30g / L sucrose added, at a temperature of 23°C, light intensity of 1500lx, and light-to-dark ratio of 10h / 14h The seed embryos are allowed to differentiate into buds, and cultivated under fluorescent lamps until the aseptic seedlings of Dendrobium candidum are about 3 to 4 cm in height, and then the aseptic seedlings of Dendrobium candidum can be induced and cultivated.

[0012] Step 2, inducing Dendrobium officinale to accumulate dendrobine: salicylic acid produced by Sigma-Aldrich Company of the United States was used. First dissolve salicylic acid with 0.1% dehydrated alcohol solution, be mixed with concentration and be the salicylic acid mother liquor of 5mmol / L, add salicylic acid mother liquor in the 1 / 2MS medium that contains 30g / L sucrose, to salicylic acid Acid final concentrat...

Embodiment 2

[0014] Step 1. Preparation of Dendrobium candidum tissue culture seedlings: use the seeds of Dendrobium candidum from Yandang Mountain in Zhejiang, sow them on the surface of 1 / 2MS medium with 30g / L sucrose, at a temperature of 24°C, light intensity of 1600lx, and light-dark ratio of 10h / 14h Allow the embryos to differentiate into buds. Cultivate under fluorescent lamps until the height of the aseptic seedlings of Dendrobium candidum is about 3 to 4 cm, and then the aseptic seedlings of Dendrobium candidum can be induced.

[0015] Step 2, inducing Dendrobium officinale to accumulate dendrobine: salicylic acid produced by Sigma-Aldrich Company of the United States was used. First dissolve salicylic acid with 0.1% dehydrated alcohol solution, be mixed with concentration and be the salicylic acid mother liquor of 5mmol / L, add salicylic acid mother liquor in the 1 / 2MS medium that contains 30g / L sucrose, to salicylic acid Acid final concentration is 150 μ mol / L, then the dendrobiu...

Embodiment 3

[0017] Step 1. Preparation of Dendrobium candidum tissue culture seedlings: Use the seeds of Dendrobium candidum from Yandang Mountain in Zhejiang, sow them on the surface of 1 / 2MS medium with 30g / L sucrose, at a temperature of 25°C, light intensity of 1700lx, and light-to-dark ratio of 10h / 14h Allow the embryos to differentiate into buds. Cultivate under fluorescent lamps until the height of the aseptic seedlings of Dendrobium candidum is about 3 to 4 cm, and then the aseptic seedlings of Dendrobium candidum can be induced.

[0018] Step 2, inducing Dendrobium officinale to accumulate dendrobine: salicylic acid produced by Sigma-Aldrich Company of the United States was used. First dissolve salicylic acid with 0.1% dehydrated alcohol solution, be mixed with concentration and be the salicylic acid mother liquor of 5mmol / L, add salicylic acid mother liquor in the 1 / 2MS medium that contains 30g / L sucrose, to salicylic acid Acid final concentration is 155 μ mol / L, then the dendro...

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Abstract

The invention provides a method for producing dendrobine by inducing dendrobium candidum by salicylic acid. The method comprises the following steps: (1) planting the dendrobium candidum seeds on the surface of a 1 / 2MS culture medium; culturing under the condition that the temperature is 23-25 DEG C, the light intensity is 1500-1700lx and the light-dark ratio is 10h / 14h to obtain a dendrobium candidum tissue culture seedling; and (2) dissolving salicylic acid by 0.1% anhydrous ethanol solution to prepare salicylic acid mother liquor with the concentration of 5mmol / L; adding the salicylic acid mother liquor into the 1 / 2MS culture medium till the final concentration of salicylic acid is 145-155 mu mol / L; then, transferring the dendrobium candidum tissue culture seedling to the 1 / 2MS culture medium with salicylic acid and culturing for 29-31 days under the condition that the temperature is 26-28 DEG C, the relative humidity is 66-68%, the light intensity is 1800-2000lx and the light-dark ratio is 12h / 12h. According to the method provided by the invention, by applying small amount of salicylic acid in tissue culture, the accumulation efficiency of dendrobine in dendrobium candidum can be improved to a great extent, the investment is small, the effect is obvious, and the method is convenient and feasible.

Description

technical field [0001] The invention provides a method for using salicylic acid to induce dendrobium officinale to produce dendrobine, which belongs to the field of biotechnology. Background technique [0002] Alkaloids are one of the most important physiologically active components of Dendrobium plants, and their pharmacological activity has a certain relationship with the content of alkaloids. Studies on the biological function and pharmacological effects of Dendrobium officinale have shown that it is a safe and healthy natural active product, which mainly has the functions of enhancing the body's immunity, anti-cancer, lowering blood pressure, and sedative and analgesic. [0003] Dendrobium officinale has low reproductive rate, slow growth, over-harvesting and utilization, and damage to the ecological environment under natural conditions, which lead to the endangerment of wild resources and limit the production and further development and utilization of dendrobium. With ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 袁佐清张建勇刘涛林聪
Owner SHANDONG UNIV OF TECH
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