A method for inducing oil in filamentous algae

A filamentous algae and oil technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of limited oil accumulation capacity of microalgae cells, difficulty in giving full play to the oil induction effect, etc. The effect of cumulative efficiency

Active Publication Date: 2020-08-14
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When microalgae cells are subjected to oil induction, oil inducers such as nutrient factors, environmental factors or chemical substances are generally added to the microalgae culture medium to promote the microalgae cells to produce more oil, but the oil inducers are often limited. Due to the oil accumulation ability of microalgae cells, it is difficult to fully exert the oil-inducing effect

Method used

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  • A method for inducing oil in filamentous algae
  • A method for inducing oil in filamentous algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The first step is to inoculate the Xanthophyll algae species into a 250mL Erlenmeyer flask, and add 100mL of heterotrophic medium (the preparation method of the heterotrophic medium includes: adding glucose aqueous solution in the BG11 medium, and the glucose is calculated as per 1LBG11 medium Add 10g of glucose to the medium), then put it into a shaker and carry out dark shaking culture at a shaking mode of 140 rpm. The algae cell concentration is 1.5g / L;

[0045] In the second step, add 1mol / L hydrochloric acid to the first microalgae liquid, so that the pH value of the first microalgae liquid=3, thereby controlling the yellow hair algae in the first microalgae liquid to start to break under the condition of pH value=3 ;

[0046] Sampling and checking the number of algal filaments every 6 hours: the specific method includes: shake the first microalgae liquid, then draw 20 μL of the first microalgae liquid from the first microalgae liquid and place it on a glass slide...

Embodiment 2

[0057] The first step is the same as the first step in Embodiment 1.

[0058] In the second step, the yellow silk microalgae cells in the first microalgae liquid are collected, and inoculated to the heterotrophic medium without phosphorus and magnesium elements (the preparation method of the heterotrophic medium comprises: in the absence of phosphorus and magnesium Add glucose aqueous solution to the elemental BG11 medium, and add 10 g of glucose per 1 L of BG11 medium without phosphorus and magnesium elements, and the total mass concentration of phosphorus and magnesium in the heterotrophic medium is the same as that of the heterotrophic medium in the first step. 10% of the total mass concentration of phosphorus and magnesium contained in the culture medium), the second microalgae liquid is obtained, the Erlenmeyer flask where the second microalgae liquid is located is sealed, and the second microalgae liquid is controlled to be in an airtight state, and Continue to carry out...

Embodiment 3

[0069]The first step is the same as the first step in Embodiment 1.

[0070] In the second step, the triangular flask where the first microalgae liquid is placed is airtight, and the first microalgae liquid is controlled to be in an airtight state, and continues to shake and cultivate in the dark at 140 rpm in a shaker to reduce the first microalgae liquid. When the dissolved oxygen content in the microalgae liquid reaches 5%, the Xanthophyllium in the first microalgae liquid begins to break; take samples every 6 hours to check the number of algae filaments under the microscope. For the specific method, see the second step of Example 1.

[0071] In the third step, when the first microalgae liquid is controlled to remain in an airtight state for 24 hours, the percentage of the number of algae growth stages in the first microalgae liquid to the initial number of algae filaments is equal to 60%, and the remaining algae filaments are shortened to the first 1 / 4-1 / 3 of the initial l...

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Abstract

The invention discloses a filamentous algae grease inducting method, and relates to the technical field of biodiesel. The grease accumulation efficiency of microalgae cells is improved, so that the grease induction effect is fully developed. The method comprises controlling the trichome fracture condition of filamentous algae in a filamentous algae nutrient solution to make filamentous algae in the filamentous algae nutrient solution to be broken into hormogons in such condition; and controlling the grease inducting condition of filamentous algae in a filamentous algae nutrient solution, and performing grease induction on filamentous algae in the filamentous algae nutrient solution according to the grease inducting condition. The provided filamentous algae grease inducting method is applied to extract biodiesel.

Description

technical field [0001] The invention relates to the technical field of biodiesel, in particular to a method for inducing filamentous algal oil. Background technique [0002] The main component of microalgae oil is triglyceride, which is an ideal raw material for the preparation of biodiesel. Compared with biodiesel extracted from oily crops and animal fat, the production cycle of biodiesel produced by high-efficiency microalgae is short and the growth rate is fast. It does not occupy arable land, can use seawater and industrial wastewater, and can be produced throughout the year. [0003] At present, the key technology of using microalgae to produce biodiesel is how to effectively induce oil in microalgae cells to increase the oil production of microalgae. When microalgae cells are subjected to oil induction, oil inducers such as nutrient factors, environmental factors or chemical substances are generally added to the microalgae culture medium to promote the microalgae cell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/6463Y02E50/10
Inventor 冯倩白雪梅王琳
Owner ENN SCI & TECH DEV
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