Microwave extraction separation and purification method of pea polysaccharide

A technology for separation and purification of peas, which is applied in the field of microwave extraction, separation and purification of pea polysaccharides, can solve the problems of low average extraction rate and purity, limited application of pea polysaccharides, and few methods for extraction, separation and purification of pea polysaccharides, and achieves the effect of improving the purity.

Active Publication Date: 2014-09-03
敖汉旗北方粮仓农产品批发市场有限公司
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Problems solved by technology

There are few extraction, separation and purification methods for pea polysaccharides, and the average extr...

Method used

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  • Microwave extraction separation and purification method of pea polysaccharide

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Embodiment 1

[0012] (1) Take 500g of fresh peas, and crush them with a grinder; (2) Extract the crushed peas with 20wt% ethanol water with a solid-liquid ratio of 1:5, microwave power of 200w, and microwave The extraction time is 3 hours, and the number of extractions is 3 times; (3) After the extraction, the extract is centrifuged at a speed of 4000 rpm for 20 minutes, and the upper supernatant is collected after centrifugation; (4) The supernatant is concentrated, Concentrate until the weight of the concentrate is 20% of the weight of the original supernatant, and after concentration, cool the concentrate to room temperature; (5) pass the concentrate through D3520 macroporous adsorption resin, the sample loading speed is 1.5BV / h, and the elution speed is optimal 2BV / h, collect the effluent, concentrate the effluent until pea crude polysaccharide is obtained; (6) purify the pea crude polysaccharide with ethanol, the weight ratio of ethanol to crude polysaccharide is 1:3, after purification...

Embodiment 2

[0014] (1) Take 300g of fresh peas, and crush them with a grinder; (2) Extract the crushed peas with 20wt% ethanol water with a solid-liquid ratio of 1:7, microwave power of 200w, and microwave The extraction time is 3 hours, and the number of extractions is 3 times; (3) After the extraction, the extract is centrifuged at a speed of 4000 rpm for 20 minutes, and the upper supernatant is collected after centrifugation; (4) The supernatant is concentrated, Concentrate until the weight of the concentrate is 20% of the weight of the original supernatant, and after concentration, cool the concentrate to room temperature; (5) pass the concentrate through D3520 macroporous adsorption resin, the loading speed is preferably 2BV / h, and the elution speed is preferably 1.5BV / h, collect the effluent, concentrate the effluent until pea crude polysaccharide is obtained; (6) purify the pea crude polysaccharide with ethanol, the weight ratio of ethanol to crude polysaccharide is 1:5, and purify ...

Embodiment 3

[0016] (1) Take 400g of fresh peas, and crush them with a grinder; (2) Extract the crushed peas with 20wt% ethanol water with a solid-liquid ratio of 1:10, microwave power of 200w, and microwave The extraction time is 3 hours, and the number of extractions is 3 times; (3) After the extraction, the extract is centrifuged at a speed of 4000 rpm for 20 minutes, and the upper supernatant is collected after centrifugation; (4) The supernatant is concentrated, Concentrate until the weight of the concentrate is 20% of the weight of the original supernatant, and after concentration, cool the concentrate to room temperature; (5) pass the concentrate through D3520 macroporous adsorption resin, the loading speed is preferably 2BV / h, and the elution speed is preferably 2BV / h, collect the effluent, concentrate the effluent until pea crude polysaccharide is obtained; (6) purify the pea crude polysaccharide with ethanol, the weight ratio of ethanol to crude polysaccharide is 1:4, and purify t...

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Abstract

The invention relates to a microwave extraction separation and purification method of pea polysaccharide. The microwave extraction separation and purification method comprises the following steps: (1) preparing fresh peas and grinding the peas by using a grinder; (2) carrying out microwave extraction on the ground peas by using 20wt% ethyl alcohol water for three times; (3) carrying out centrifugal treatment on an extracting solution, and then collecting liquid supernatant at the upper layer; (4) concentrating the liquid supernatant till the weight of a concentrate is 20% of the weight of the original liquid supernatant, and then cooling a concentrated solution to a room temperature; (5) enabling the concentrated solution to pass through D3520 macroporous adsorption resin, collecting effluent liquid, and concentrating till obtaining pea crude polysaccharide; and (6) purifying the pea crude polysaccharide by using ethyl alcohol, and drying so as to obtain the pea polysaccharide. The solution extraction rate of the pea polysaccharide prepared by the method is 12wt% to 16wt%, and after separation and purification, and the yield of the pea polysaccharide still can reach 76%-82%.

Description

technical field [0001] The invention relates to a method for extraction, separation and purification of polysaccharides, in particular to a method for microwave extraction, separation and purification of pea polysaccharides. Background technique [0002] Polysaccharide is an active ingredient, which is obtained by condensation and dehydration of monosaccharides, disaccharides or other sugars. Most polysaccharides have good application value, such as immune regulation, antibacterial, hypoglycemic and other effects. Polysaccharides are more common in plants, and the average content can reach 10wt% to 30wt%. Because of its good medicinal value, polysaccharides in various plants are a research hotspot in the corresponding field. [0003] There are few relevant studies on pea polysaccharides at present, and currently known pea polysaccharides have anti-oxidation and hypoglycemic effects. There are few extraction, separation and purification methods for pea polysaccharides, and...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 牛士华
Owner 敖汉旗北方粮仓农产品批发市场有限公司
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