Method for cultivating matsutake hyphae and special device thereof
A technology of matsutake mycelium and culture tank, which is applied in the field of artificial cultivation of matsutake mycelia, can solve the problems of restricting the deep development and utilization of functional substances, slow growth, small scale, etc., and achieve low cost, easy operation and small volume Effect
Active Publication Date: 2016-10-26
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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Problems solved by technology
[0003]Currently, the incubators used for matsutake mycelium cultivation are all in the shape of triangular flasks or cylinders, which are difficult to separate, slow to grow, small in scale and low in yield, etc. Problems, such as the liquid culture yield of 500ml Erlenmeyer flask is only about 4g / L, although there are reports about the yield of about 20g / L, but the culture volume is only 100ml scale or the culture time is as long as more than 1 month
The common problems in the artificial cultivation of matsutake mycelium are small scale, long period and low yield, which seriously restrict the field artificial propagation technology of matsutake and the deep development and utilization of functional substances, which need to be improved urgently
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experiment example 1
[0038] Experimental example 1 The experiment of cultivating matsutake mycelia in special cultivation tanks for matsutake mycelia of the present invention
[0039] 1. Training equipment and materials
[0040] 1. Cultivation tank: adopt figure 2 The special culture tank shown (the tank volume is 10L).
[0041] 2. Preparation of mycelium for inoculation
[0042] 2.1 Cultivate with solid medium (24°C) for 60 days;
[0043] 2.2 Mince the mycelium, put it in several 500ml Erlenmeyer flasks (200mL culture solution), and culture it in a shaker (110rpm, 24°C) for more than 20 days;
[0044] 2.3 The liquid culture mycelium was crushed and quantified with a mycelium masher (13000rpm, 30s).
[0045] 3. Composition of liquid culture medium (g / L)
[0046] Carbon (C) source 30-35, nitrogen (N) source 0.7-0.8, phosphorus (P) source 0.25-0.30, potassium (K) 0.28-0.30, magnesium (Mg) 0.045-0.050, calcium (Ca) 0.45-0.50 , manganese (Mn) 0.47-0.48, pH6.5.
[0047] 2. Training method
[0...
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The invention discloses a matsutake mycelium cultivation method and a special device, belonging to the field of matsutake mycelium artificial cultivation. The special device includes: tank body (1), tank cover (2), stirring rod (3), stirring blade (4), air outlet (5), inoculation port (6), two external circulation ports (7) and (8), sampling port (9), air inlet (11); wherein the upper and middle parts of the tank body (1) are spherical, the lower part is a gentle slope type tank neck, and the whole tank body (1) is in the shape of a balloon. The invention further discloses a method for cultivating matsutake mycelium in large quantities by using the special device. The special matsutake mycelia cultivation tank of the present invention solves the problems of slow matsutake mycelium growth and difficulty in mass production. The deep utilization of metabolites has important application potential.
Description
technical field [0001] The invention relates to a method for cultivating fungi and a special device for realizing the method, in particular to a method for cultivating matsutake hyphae and a special device thereof, and belongs to the field of artificial cultivation of matsutake mycelium. Background technique [0002] Matsutake is a precious wild fungus under the forest, which has been officially included in the national second-class protected plant list. Matsutake is an ectomycorrhizal fungus that forms a trophic symbiotic relationship with pine trees. It must rely on the roots of living trees to maintain growth. Due to the harsh requirements for growth conditions, matsutake cannot be cultivated artificially. Due to the destruction of the forest ecological environment and the disorderly and vicious collection, the biosphere in which it grows is shrinking day by day, and the resources are increasingly scarce; therefore, the demand for the artificial propagation of matsutake a...
Claims
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IPC IPC(8): A01G1/04
Inventor 朴春根李永郭民伟薛寒田国忠汪来发王曦茁林彩丽
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY