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Pig eperythrozoonosis vaccine and preparation method thereof

A technology of porcine eperythrozoon and porcine eperythrocytes, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of no protective effect and little clinical significance, and achieve low cost and high production process And the effect of simple structure and good immune response

Active Publication Date: 2014-09-10
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The subunit vaccines studied by Lu Zhou, Zheng Xiuhong, etc. have a certain effect on mice, but whether the vaccine can produce immune protection in other animals remains to be further studied, and its clinical significance is very little; the vaccines studied by Hoelzle K et al. Produces strong levels of immunity but is not protective

Method used

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  • Pig eperythrozoonosis vaccine and preparation method thereof
  • Pig eperythrozoonosis vaccine and preparation method thereof
  • Pig eperythrozoonosis vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] The preparation of the vaccine adopts a more effective and convenient inactivation method, and its detection is also more intuitive and feasible.

[0019] The immune effect and protective effect of the vaccine can be tested through the combination of laboratory and clinical methods, which provides a reliable basis for future clinical application and improves the feasibility of the research.

Embodiment 1

[0020] Embodiment 1, the preparation of vaccine comprises the following steps:

[0021] 1. The cultivation of porcine Eperythrozoon. The erythrocytes infected with porcine Eperythrozoon were inoculated into cell culture flasks and placed at 37°C with 5% CO 2 After about 72 hours of culture in an incubator, the red blood cells hemolyzed and turned into ghosts with worms, and then cultured continuously for 30 days to fully reduce the components of the red blood cell membrane, and use ultrasonic waves to dissociate the ghosts of porcine eperythrocytic bodies into single pathogens, count them with a red blood cell counting board and continue After culture, the proliferation reached the peak at 48 hours, and the number of proliferation reached 30-40%, and then decreased. In the field of vision, 2-6 proliferating Eperythrozoon porcine can be seen, and then ultrasonicated into a single antigen for detection The number of them reaches 2.87x10 9 cells / mL for vaccine preparation. Med...

Embodiment 2

[0025] Embodiment 2, the evaluation of vaccine effect

[0026] 1. Safety evaluation.

[0027] The prepared vaccine was prepared as described above. The prepared vaccine was injected into 20 Kunming mice, each with 0.2 mL intramuscularly. The diet and mental state of the mice were observed. No significant changes were found, and no mice died at 21 days.

[0028] The prepared vaccine was injected into 10 sows, 10 suckling piglets, 10 nursery pigs, and 10 fattening pigs. The mental state, respiration, and pulse of the pigs were observed to be normal, and no death occurred in 21 days.

[0029] 2. Evaluation of immune effect in mice

[0030] Thirty Kunming mice were randomly divided into 2 groups, 15 in each group, injected intramuscularly with 0.2 mL of the prepared vaccine, and boosted once 7 days later. The control group was injected with the same dose of normal saline. The mental state of the mice in the control group and the immunized group was observed, and the mice were ki...

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Abstract

The invention discloses pig eperythrozoonosis vaccine and a preparation method thereof. The preparation method comprises preparation processes such as cultivation of pig eperythrozoon, inactivation of pig eperythrozoon, emulsification of vaccine adjuvant and the like, thereby obtaining the inactivated pig eperythrozoonosis vaccine. The physicochemical properties such as the stability and the viscosity of the vaccine are detected, the security of the vaccine is detected through mice, piglets are subjected to immunological experiment, immunity detection on specificity immunity and non-specificity immunity is performed, and the detection on the protection function of the inactivated vaccine for animals and the clinical effects of the vaccine is performed. The vaccine has the advantages that the clinical and experiment data show that the vaccine has the security and the protection capability, further has the characteristics of simple preparation process, easiness to obtain, low cost and the like, and can be applied to clinical veterinarian in large scale. The vaccine is significant for prevention on pig eperythrozoon, and has important clinical meaning in reducing the pig industry loss caused by the pig eperythrozoonosis.

Description

technical field [0001] The invention belongs to the technical field of livestock vaccines, in particular to a vaccine for preventing porcine eperythrozoonosis and a preparation method thereof, which is an effective and safe vaccine in the field of veterinary medicine. Background technique [0002] Porcine Eperythrozoonosis is caused by Eperythrozoon porcine (M.suis) and has the characteristics of host specificity. It is a zoonotic infectious disease that often causes symptoms such as fever, hemolysis, and jaundice. The disease can cause weakness and anemia in newborn piglets and weaned piglets, slow growth in finishing pigs, reproductive failure, low conception rate, anoestrus, abortion and stillbirth in sows and other comprehensive symptoms. It not only leads to the reduction of the quality and output of livestock products, but also causes a large number of pigs to die. What is more serious is that the immune function of pigs infected with Eperythrozoonosis is greatly redu...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61P31/04C12N1/20C12R1/35
Inventor 贾杏林唐丽李晓云
Owner HUNAN AGRICULTURAL UNIV
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