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A SNP molecular marker primer for the major QTL site of pear peel smoothness and its application

A technology of marking primers and smoothness, which is applied in the field of molecular genetics and breeding, to achieve the effects of linkage and high reliability, and improve accuracy and efficiency

Active Publication Date: 2016-08-17
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on QTL mapping of quantitative traits of pears is still in its infancy. Existing studies are mainly aimed at some diseases or growth traits, QTL mapping of pear peel smoothness traits and detection of polymorphic expression of peel smoothness at QTL sites The research on the development and application of differential SNP molecular markers has not been reported yet

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  • A SNP molecular marker primer for the major QTL site of pear peel smoothness and its application
  • A SNP molecular marker primer for the major QTL site of pear peel smoothness and its application
  • A SNP molecular marker primer for the major QTL site of pear peel smoothness and its application

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Embodiment 1

[0029] Molecular markers linked to major QTLs for smoothness of pear peel were obtained by the following methods:

[0030] a) Using 'August Red' and 'Dangshansu pear' (variety is known and public, see literature: Zhang Ruiping et al., Construction of pear AFLP marker genetic map and QTL mapping of fruit-related traits, Acta Horticultural Science, 2011, 38(10): 1991–1998) to obtain 102 F 1 Progeny single plant.

[0031] b) Using RADseq method for high-throughput sequencing, analyze 'August Red' and 'Dangshan Suli' and their offspring, and statistically analyze the genetic type of polymorphic sites in the offspring population, using χ 2 The test analyzes whether the segregation of each marker conforms to the Mendelian genetic segregation ratio of 3:1 or 1:1.

[0032] c) Molecular genetic linkage maps of 'August Red' and 'Dangshan Suli' were constructed using Joinmap4.0 analysis software. Import the polymorphic marker sites obtained in step b) into Joinmap4.0 according to the ...

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Abstract

The invention discloses SNP (single nucleotide polymorphism) molecular marker primers for pear peel smoothness main effect QTL (quantitative trait locus) site and application thereof. In the closely linked SNP marker Pybd02-010 of the pear peel smoothness main effect QTL site, the sequence of a forward primer is as shown in the SEQ ID NO.1, and the sequence of a reverse primer is as shown in the SEQ ID NO.2. The SNP marker for pear peel smoothness is identified by utilizing the specific primers based on a high resolution melting curve, the peel smoothness can be well typed, namely the difference of transcript-derived peel smoothness on the QTL site qSmooth is detected. The pear peel smoothness main effect QTL molecular marker can be used in molecular marker-assisted selective breeding of pear peel smoothness characters, and has important theoretical and practical guiding meaning in speeding up the genetic improving process of pear varieties and improving breeding selection efficiency.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, and provides a SNP molecular marker primer for the main effect QTL site of pear peel smoothness and an application thereof. Background technique [0002] my country is one of the most important origins of Pyrus L. in the world, with rich genetic diversity. The cultivars of pears are widely distributed, and they are cultivated except in Hainan Province and Taiwan Province. Pear fruit has high nutritional value, sweet and juicy, and is deeply loved by consumers. The appearance quality of the fruit is one of the important factors to measure the fruit grade, and the smoothness of the peel is the first intuitive factor to evaluate the fruit appearance quality. With the improvement of people's living standards, people have higher and higher requirements for fruit quality. Fruits with rough fruit surface, chapped skin, fruit rust and small black spots are gradually eliminated from the high-qu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 吴俊张绍铃李雷廷孙江妹张全军刘伦齐开杰
Owner NANJING AGRICULTURAL UNIVERSITY