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A mechanically stimulated bioreactor system imitating a physiological environment

A bioreactor, a technology of imitating physiological environment, which is applied in the field of bioreactor system of imitating physiological environment mechanical stimulation

Inactive Publication Date: 2016-01-20
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention is not suitable for in vitro culture of engineered bone tissue

Method used

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  • A mechanically stimulated bioreactor system imitating a physiological environment
  • A mechanically stimulated bioreactor system imitating a physiological environment
  • A mechanically stimulated bioreactor system imitating a physiological environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1——Case of implementation of pressure mode

[0036] Please refer to figure 1 with figure 2 , the simulated physiological environment mechanical stimulation type bioreactor system described in this embodiment includes CO 2Incubator 30, main body components, circulation flow components and control components.

[0037] The CO 2 The working environment of the incubator 30 is usually: 100% humidity, CO 2 The concentration is 5% and the temperature is 37°C.

[0038] The body part is placed in the CO 2 In the incubator 30, the main parts include an electric cylinder 1, a sealing cover 2, a motor frame 3, a dust cover 4, a connecting rod 5, a force sensor 6, an adapter plate 7, a fixing sleeve 10, a base 13, a fixing pin 16, two The rod 14, the culture chamber 12 and the loading member are secured.

[0039] The motor frame 3 is connected and fixed to the base 13 at the bottom through four upright support rods 8 to form the basic support of the main body componen...

Embodiment 2

[0048] Example 2——Pull mode implementation case

[0049] The system structure of this embodiment is basically the same as that of Embodiment 1, the difference lies in the loading component. see Figure 4 , the loading member of the present embodiment is a pull mode; its upper part includes a pull rod 18, a threaded chuck 19 and a through hole chuck 20, and the upper end of the pull rod 18 forms a rigid connection with the adapter plate 7 through a coupling 17, The threaded chuck 19 and the through-hole chuck 20 are installed on the lower end of the pull rod 18 stretching into the cultivation chamber 12, and are connected with the threaded chuck 19 through the through-hole chuck 20 with a screw, and the through-hole can be made by rotating the screw. Collet 20 cooperates with threaded collet 19 to clamp one end of the engineered tissue together; the lower part of the loading member includes a pull mode platform 22 and a lower end clamp 21 fixed on the pull mode platform 22, th...

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Abstract

The invention discloses a physiological environment-imitating mechanical stimulation type biological reactor system. The physiological environment-imitating mechanical stimulation type biological reactor system comprises a CO2 incubator, a main body part, a circulatory flowing part and a control part, wherein a working environment for the CO2 incubator is as follows: 100% humidity, CO2 concentration of 5% and a temperature of 37 DEG C; the main body part is placed in the CO2 incubator, comprises a culturing room used for placing engineered tissues and a loading component used for applying force to the engineered tissues; the loading component extends into the culturing room filled up with culture liquid; the control part is placed on the outer surface of the CO2 incubator and is connected with the main body part; the control part controls the loading component to apply the corresponding stretching force load or compressing force load to the engineered tissues according to preset change laws; the circulatory flowing part is connected with the culturing room; the culture liquid forms circulatory flow between the culturing room and the circulatory flowing part; the engineered tissues bear stimulation of fluid shearing force produced by the flow of the culture liquid. The physiological environment-imitating mechanical stimulation type biological reactor system disclosed by the invention has the multifunctional characteristic, so that the engineered tissues can bear shearing force of a more like human physiological environment and mechanical stimulation action of stretching or compressing in the system.

Description

technical field [0001] The invention relates to a tissue engineering bioreactor system in the field of biomedical engineering, in particular to a mechanically stimulated bioreactor system imitating a physiological environment. Background technique [0002] Bone tissue is a dynamic organ, its structure and function largely depend on the mechanical environment in which it is located, and adaptive changes occur according to the mechanical environment. In the physiological state, bone tissue is constantly remodeling and shaping to adapt to the physical support required for body activities, so as to meet the special needs of different individuals for movements or postures. Excessive and inappropriate external stimulation can easily lead to damage to human bones. Clinically, long-term bed rest or immobilization can lead to osteoporosis in the corresponding parts. Appropriate mechanical stimulation can promote the growth and reconstruction of human bones. For example, reasonable ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/36C12M1/34
CPCC12M35/04C12M41/14
Inventor 李祥冯辰栋
Owner SHANGHAI JIAOTONG UNIV
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