Multiplex PCR (polymerase chain reaction) primers and method for identifying Panthera pardus, and application thereof in identifying Panthera pardus

A golden leopard, multiple technology, applied in the fields of biochemical equipment and methods, recombinant DNA technology, microbial determination/inspection, etc., can solve the problems of time-consuming and labor-intensive, and achieve the reduction of sampling difficulty, economical and practical identification, and simplicity. The effect of identification
CN104059979AInactive Publication Date: 2014-09-24ANHUI NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ANHUI NORMAL UNIV
Publication Date
2014-09-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses multiplex PCR primers for identifying Panthera pardus, which comprise a primer pair 1 disclosed as SEQ ID NO:1 and SEQ ID NO:2 and a primer pair 2 disclosed as SEQ ID NO:3 and SEQ ID NO:4. The invention also discloses a method for identifying Panthera pardus by utilizing multiplex PCR, which comprises the following steps: extracting total DNA (deoxyribonucleic acid) of a sample to be detected, and carrying out PCR amplification on the total DNA; and carrying out agarose gel electrophoresis detection on the amplified product, wherein the multiplex PCR primers are the multiplex PCR primers in the invention, and the sample to be detected is judged to be from Panthera pardus if two strips appear in the lane. The invention also discloses application of the multiplex PCR primers or identification method in identifying Panthera pardus. The two pairs of primers are designed to achieve the goal of identifying Panthera pardus in a simple, feasible, economical and practical way.
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Description

technical field

[0001] The invention relates to the field of biological detection, in particular to a multiple PCR primer for identifying leopards, a method for identifying leopards using the multiple PCR primers, and their application in identifying leopards. Background technique

[0002] Accurate species identification based on taxonomy is a necessary prerequisite for human cognition of nature and sustainable development. It provides a knowledge and theoretical basis for the conservation of biodiversity, the sustainable use of species resources, and the development of new products from biological sources. For the identification of species, traditional methods mainly use morphological evidence. However, some shortcomings of this method have also caused limitations in the protection of endangered animals, such as phenotypic plasticity and genetic variability, inability to distinguish cryptic taxa, and restrictions by biological sex and developmental stage. In the practice ...

Claims

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