A kit for detecting circulating tumor cells
A tumor cell and kit technology, applied in the field of kits for detecting circulating tumor cells, can solve the problems of missing important disease information of CTCs, high missed detection rate, low sensitivity, etc., so as to reduce the detection cost and price, low cost and low cost low cost effect
Active Publication Date: 2016-08-17
南京至泰生物医药科技有限公司
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Problems solved by technology
The most representative one is to use the diameter of general tumor cells to be larger than that of normal cells to separate and detect CTCs. For example, membrane filtration separation technology is to design an 8 μm hole array on the filter membrane. It is assumed that the diameter of CTCs is generally above 10 μm. And the cell membrane has poor elasticity, so CTCs can be separated; but the diameter of some normal white blood cells in the blood is greater than 8 μm, and some exceed 10 μm; Small, and studies have found that tumor cells can easily pass through channels with a diameter of 5 μm. Therefore, the method of using the physical characteristics of tumor cells to separate and detect CTCs has been greatly questioned
[0004] To sum up, the current detection techniques and methods for CTCs have relatively large defects, such as low sensitivity and high missed detection rate, and generally only detect the number of CTCs, and seldom detect the expression of tumor-associated molecules on CTCs. The important disease information carried by CTCs cannot meet the requirements of clinical research and application. Therefore, there is an urgent need to develop an efficient and low-cost CTCs detection platform that can detect the number of all CTCs in blood samples and analyze the expression of tumor-related molecules at the same time. In order to provide more accurate information for clinical diagnosis, curative effect monitoring and prognosis judgment of tumors
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[0019] Circulating tumor cells (CTCs) are the key to tumor metastasis and recurrence, and have important research significance and application value. The current technical method of separating and detecting CTCs based on molecular markers on the surface of CTCs and their own physical properties has certain defects in principle, resulting in poor detection sensitivity and low specificity. The present invention uses ordinary polystyrene or silicon dioxide materials (such as glass slides, quartz, silicon wafers, etc.) Physically enrich all the cells in the suspension to the surface of the substrate, and then use tumor-associated antibodies to detect the cells to identify tumor cells. In experiments, it was found that the substrate can enrich almost all tumor cells artificially mixed into the blood of healthy people within 30 minutes. This method subverts the existing principle of CTCs detection, and is simple, efficient and low in cost.
[0020] 1. Preparation of positively cha...
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Abstract
The invention discloses a kit detecting circulating tumor cells. According to the kit, the cells are absorbed by adopting a substrate material coating positive charges, and the tumor cells are marked by using a tumor associated antibody, and thus the detection on the tumor cells is realized. The kit disclosed by the invention is low in detection cost, simple, efficient, and easy to popularize.
Description
technical field [0001] The invention belongs to the field of biotechnology, in particular to a kit for detecting circulating tumor cells. Background technique [0002] Circulating tumor cells (CTCs) are the key to tumor metastasis and recurrence. Tumor cells shed from the primary tumor, invade the blood circulation, become CTCs, evade the body's immunity, settle in distant organs or primary organs, and form metastatic recurrence lesions . Studies have found that detecting the number of CTCs is helpful for early detection of colon cancer, for judging the prognosis of patients with liver cancer and breast cancer, and evaluating the effect of chemotherapy for patients with colon cancer and lung cancer; when CTCs appear epithelial-mesenchymal transition (EMT ), and overexpression of epithelial cell adhesion molecule (EpCAM), it often indicates that the prognosis of tumor patients is poor. Therefore, the detection of CTCs is vividly called "liquid biopsy", which has important cl...
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IPC IPC(8): G01N33/574
CPCG01N33/5436G01N33/574
Inventor 万源郑勤王礼学
Owner 南京至泰生物医药科技有限公司