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A method for increasing chlorogenic acid content in chrysanthemum inflorescences

A technology of chlorogenic acid and inflorescence, which is applied in botany equipment and methods, tea treatment before extraction, plant gene improvement, etc., can solve the problems of long promotion and use period, poor controllability, cumbersome steps, etc., and achieve the goal of avoiding chlorogenic acid The effect of content attenuation, low cost and simple steps

Inactive Publication Date: 2016-04-13
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method that can effectively increase the content of chlorogenic acid in chrysanthemum inflorescences, so as to solve the problems of cumbersome steps, poor controllability, low efficiency, long promotion and use period and high cost in the existing methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Place the picked fresh chrysanthemum inflorescences in the cultivation room, and irradiate with ultraviolet light UV-B under the condition of indoor temperature of 25°C and humidity of 60%, with an irradiation intensity of 200μw / cm 2 , the irradiation time is 30min;

[0030] (2) Transfer the inflorescences irradiated in step (1) into an incubator, and culture them in the dark for 12 hours under the conditions of a temperature of 25°C and a humidity of 60% in the incubator;

[0031] (3) Put the inflorescences treated in step (2) in a dryer, fix them at 105°C for 10 minutes, and then dry them at 55°C until they reach a constant weight.

Embodiment 2

[0033] (1) Place the picked fresh chrysanthemum inflorescences in the cultivation room, and irradiate them with ultraviolet light UV-B under the conditions of indoor temperature of 20°C and humidity of 70%, and the irradiation intensity is 200μw / cm 2 , the irradiation time is 120min; when the irradiation period reaches 60min, the irradiation surface and the backlight surface of the inflorescence are turned over;

[0034] (2) Transfer the inflorescences irradiated in step (1) into an incubator, and culture in the dark for 12 hours under the conditions of a temperature of 20°C and a humidity of 70% in the incubator;

[0035] (3) Put the inflorescences treated in step (2) in a dryer, fix them at 100°C for 8 minutes, and then dry them at 50°C until they reach a constant weight.

Embodiment 3

[0037] (1) Place the picked fresh chrysanthemum inflorescences in the cultivation room, and irradiate with ultraviolet light UV-B under the condition of indoor temperature of 22°C and humidity of 80%, and the irradiation intensity is 200μw / cm 2 , the irradiation time is 15min;

[0038](2) Transfer the inflorescences irradiated in step (1) into an incubator, and culture in the dark for 12 hours under the conditions of a temperature of 22°C and a humidity of 80% in the incubator;

[0039] (3) Place the inflorescences treated in step (2) in a dryer, fix them at 102°C for 9 minutes, and then dry them at 53°C until they reach a constant weight.

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Abstract

The invention discloses a method for increasing the content of chlorogenic acid in chrysanthemum inflorescences. The method comprises the following steps: (a) putting picked fresh chrysanthemum inflorescences into a culture room, and irradiating by using ultraviolet rays UV-B at irradiation intensity of 50-600muW / cm<2> for 15-120 minutes under the conditions that the temperature is 20-25 DEG C and the humidity is 50%-80%; (b) transferring the irradiated inflorescences into a culture box and performing dark culture for 12 hours under the conditions that the temperature is 20-25 DEG C and the humidity is 50%-80% in the box; (c) performing fixation on the inflorescences processed in the step (b) in a drying machine at 100-150 DEG C for 8-10 minutes, and drying at 50-55 DEG C until the weight is constant. According to the preparation method, the process is simple, the environment-friendly effect is achieved, the safety coefficient is high, the production cycle is short, the cost is low, the benefit is high, the operation controllability is good and the chlorogenic acid is suitable for large-scale industrialized production.

Description

technical field [0001] The invention relates to a method for increasing the content of medicinal components in plants, in particular to a method for increasing the content of chlorogenic acid in chrysanthemum inflorescences. Background technique [0002] Chrysanthemum (Chrysanthemum) is a perennial herb of Compositae, with erect stems, ovate to lanceolate leaves, pinnately lobed or half-cleft, flower heads, and involucral bracts with multiple layers and different sizes. Chrysanthemum is a homologous plant of medicine and food, and its dried flower head is a commonly used traditional Chinese medicine in China. It has various effects such as treating coronary heart disease, lowering blood pressure, preventing hyperlipidemia, antibacterial, antiviral, anti-inflammatory, and anti-aging. The chemical composition of its inflorescence includes amino acids, trace elements, triterpenoids, chlorogenic acid, volatile oil, etc. Among them, chlorogenic acid is clearly specified in the 20...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06A01H1/00A23F3/06A23F3/34
Inventor 姚晓芹楚建周司超贺学礼马春辉
Owner HEBEI UNIVERSITY
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