Application of lindley eupatorium herb in preparing anti-hepatitis B virus medicaments

An anti-hepatitis B, wild horse technology, used in antiviral agents, drug combinations, pharmaceutical formulations, etc.

Active Publication Date: 2014-10-01
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

) But whether Yemachai has the effect of anti-hepatitis B virus, there is no r...

Method used

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  • Application of lindley eupatorium herb in preparing anti-hepatitis B virus medicaments
  • Application of lindley eupatorium herb in preparing anti-hepatitis B virus medicaments
  • Application of lindley eupatorium herb in preparing anti-hepatitis B virus medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation of Four Groups of Yemazhui Extracts and Chain Diterpene Compounds in Yemazhui

[0031] (1) Weigh 1000g of Yemazhui dried whole herb, cut into sections of 2-3cm, add 15 times the volume of 80% ethanol aqueous solution, reflux extraction for 2 hours, repeat twice, recover the solvent under reduced pressure at 60°C to obtain Yemazhui Ethanol extract (EUP-EtOH) 158g, dissolved in about 150ml water, adsorbed by 1800ml AB-8 macroporous adsorption resin, first eluted with 20000ml water to remove macromolecular compounds, and then eluted with 20000ml volume fraction 95% ethanol aqueous solution, The solvent was recovered under reduced pressure at 60°C to obtain 61g of macroporous resin parts; the obtained macroporous resin parts were adsorbed by 1500ml Cosmsil ODS reversed-phase silica gel filler particle size 70um reversed-phase silica gel chromatography column, and eluted with 10000ml volume fraction as 50% methanol aqueous solution, 60 The solvent was ...

Embodiment 2

[0033] Example 2: Detection of active ingredients of sesquiterpene parts, flavonoid parts and chain diterpene compounds in Yemazhui

[0034] (1) Detection of the active ingredient in the sesquiterpene part of Yemazhui (EUP-SQT): In this example, high performance liquid chromatography was used to determine its ingredients. High performance liquid chromatography (Agilent1260Infinity, Agilent Corporation of the United States), wherein mobile phase A is acetonitrile; mobile phase B is water; mobile phase conditions are shown in Table 1. Chromatographic column: YMC-PACK ODS-A (Ф4.6mm×250mm, 5μm); column temperature: 28℃; detection wavelength: 210nm; injection volume: 20μl; flow rate: 1ml min -1 .

[0035] Table 1 HPLC chromatographic analysis conditions

[0036] time (min)

Mobile phase A(%)

Mobile phase B(%)

0

30

70

5

30

70

15

32

68

25

36

64

40

36

64

[0037] Accurately weigh 6.02 ...

Embodiment 3

[0063]Example 3: Anti-hepatitis B virus HBV experiment of four kinds of Yemazhui extracts and chain diterpene compounds in Yemazhui

[0064] 1. Experimental drugs and reagents: chain diterpene compounds (1) and (2) prepared in the above-mentioned embodiment 1; dimethyl sulfoxide; MTT (thiazolium blue), Shanghai Jianglai Biotechnology Co., Ltd.; reference drug, Lamy Furidin Tablets (3TC); GlaxoSmithKline Pharmaceuticals (Suzhou) Co., Ltd.; cell culture medium DMEM, growth fluid, prepared, containing 10% fetal bovine serum, 380 μg / ml G418, 0.03% glutamine, penicillin, streptamine Mycin each 100μg / ml, etc.;

[0065] 2. Experimental equipment: ELISA kits for detecting hepatitis B surface antigen HBsAg and hepatitis B core antigen HBeAg; incubators; 24-well plates, etc.

[0066] 3. Cell line: 2.2.15 cell line of Hep G2, Ministry of Education / Ministry of Health Key Laboratory of Medical Molecular Virology, Shanghai.

[0067] 4. Experimental method:

[0068] Take the 2.2.15 cell l...

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Abstract

The invention discloses an application of lindley eupatorium herb in preparing anti-hepatitis B virus medicaments. Lindley eupatorium herb ethanol extract, lindley eupatorium herb flavone part, lindley eupatorium herb hemiterpene part, lindley eupatorium herb 70% ethanol water elution part and chain diterpene are prepared from lindley eupatorium herb and are subjected to hepatitis B virus (HBV) testing, and testing results indicate that the four extracts and the chain diterpene have the effect of remarkably inhibiting hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBeAg), and have better drug activities than those of clinical medicament lamivudine (3TC) for treating hepatitis B. The lindley eupatorium herb can be used for preparing medicaments for resisting hepatitis B virus.

Description

technical field [0001] The present invention relates to the application of Yemazhui in the preparation of anti-hepatitis B virus drugs, more specifically, Yemazhui ethanol extract, Yemazhui flavonoid parts, Yemazhui sesquiterpene parts, and Yemazhui 70% ethanol water Application of elution site and chain diterpene compound in preparation of anti-hepatitis B virus drug. Background technique [0002] Hepatitis B virus (HBV) infection is one of the major diseases affecting human health. About 300 million people in the world are chronic hepatitis B virus carriers. China is the country with the largest number of hepatitis B virus carriers, with about 93 million people. Acute hepatitis B can easily turn into chronic hepatitis, and even worsen into cirrhosis and liver cancer. At present, the domestic medicines for the treatment of chronic viral hepatitis are mainly 1, oral nucleoside (acid) chemical drugs, such as lamivudine, adefovir, entecavir, telbivudine, tenofovir, etc., but ...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61K31/352A61K31/365A61K31/22A61P31/20A61P1/16
Inventor 张健陈道峰任慧玲褚纯隽李显伦严彪吴双庆
Owner SUZHOU UNIV
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