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A kind of method of cassava tissue culture seedling strengthening and rooting

A technology of strong seedlings rooting and tissue culture seedlings, applied in the field of agricultural reproduction, can solve the problems of brittleness, easy breakage, water loss and wilting, etc., and achieve the effect of improving quality, increasing dry matter content, and inducing good quality

Active Publication Date: 2016-04-06
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to: in view of the above-mentioned existing problems, provide a kind of method for cassava tissue-cultured seedling strong seedling rooting, overcome traditional cassava tissue-cultured seedling in the process of seedling hardening and transplanting more brittle and easy to break, easy to lose water and wilt, etc. , improve the survival rate of cassava tissue culture seedlings transplanted, and improve work efficiency and reduce production costs

Method used

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  • A kind of method of cassava tissue culture seedling strengthening and rooting

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Embodiment 1

[0017] First, the sterile cassava tissue culture seedlings were cut into single-bud stem segments, and transferred to medium A for proliferation and subculture. Medium A was MS+0.01mg / LBA+0.02mg / LNAA+30g / L The semi-solid medium of sucrose has a pH value of 5.8 and is cultivated under the conditions of a culture temperature of 27±1°C, a light intensity of 1500lx, and a light time of 12h / d, and the culture period is 40d. Then, the single-bud stem section of cassava tissue culture seedlings after the proliferation and subculture is transferred to medium B for cultivation, and medium B is a semi-solid medium of MS+0.01mg / LNAA+40g / L sucrose, pH The value is 5.8, the culture temperature is 27±1°C, the light intensity is 1500lx, the light time is 12h / d, and the culture period is 40d. Finally, the rooted tissue cultured seedlings of sterile strong seedlings up to 5 cm were moved from the cultivation room into the greenhouse for hardening for 7 days, and then the hardened tissue cultur...

Embodiment 2

[0021] First, the sterile cassava tissue culture seedlings were cut into single-bud stem segments, and transferred to medium A for proliferation and subculture. Medium A was MS+0.01mg / LBA+0.02mg / LNAA+30g / L The semi-solid medium of sucrose has a pH value of 5.8 and is cultivated under the conditions of a culture temperature of 27±1°C, a light intensity of 1500lx, and a light time of 12h / d, and the culture period is 40d. Then, the cassava tissue culture seedling single-bud stem section after the proliferation and subculture is transferred to medium B for cultivation, and medium B is a semi-solid medium of MS+0.01mg / LNAA+45g / L sucrose, pH The value is 5.8, the culture temperature is 27±1°C, the light intensity is 1500lx, the light time is 12h / d, and the culture period is 40d. Finally, the rooted tissue cultured seedlings of sterile strong seedlings up to 5 cm were moved from the cultivation room into the greenhouse for hardening for 7 days, and then the hardened tissue cultured s...

Embodiment 3

[0025] First, the sterile cassava tissue culture seedlings were cut into single-bud stem segments, and transferred to medium A for proliferation and subculture. Medium A was MS+0.01mg / LBA+0.02mg / LNAA+30g / L The semi-solid medium of sucrose has a pH value of 5.8 and is cultivated under the conditions of a culture temperature of 27±1°C, a light intensity of 1500lx, and a light time of 12h / d, and the culture period is 40d. Then, the single-bud stem section of the cassava tissue culture seedling after the proliferation and subculture is transferred to the culture medium B in the culture room for cultivation, and the culture medium B is half of MS+0.01mg / LNAA+50g / L sucrose The solid medium has a pH value of 5.8, a culture temperature of 27±1°C, a light intensity of 1500lx, a light time of 12h / d, and a culture period of 40 days. Finally, the rooted tissue cultured seedlings of sterile strong seedlings up to 5 cm were moved from the cultivation room to the greenhouse for hardening for...

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Abstract

The invention discloses a method for promoting rooting of strong seedlings of cassava tissue culture seedlings, relates to the field of agricultural propagation methods. The method comprises the steps of successive transfer culture of tissue culture seedlings, rooting culture of strong seedlings of the tissue culture seedlings and transplant and survival of hardening-seedlings of the rooting tissue culture seedlings, wherein the successive transfer culture of the tissue culture seedlings comprises the steps as follows: germfree tissue culture seedlings are sliced into single bud stems, and then the stems are transferred to a culture medium A for multiplication successive transfer culture; the rooting culture of the strong seedlings of the tissue culture seedlings comprises the steps as follows: multiplication successive transfer cultured cassava tissue culture seedling single bud stems are then transferred to a culture medium B of a culture room for culture at the culture temperature of 26 to 28 DEG C, with the illumination intensity of 1500 to 2000 lx, within the illumination time of 12 h / d and the culture period of 40 d, wherein the culture medium B is a semisolid medium of cane sugar with MS (or 1 / 2 MS) + 0.01-0.02 mg / LNAA + 40-50 g / L and with the PH value of 5.8. The method overcomes the problems that the cassava tissue culture seedlings are fragile and easy to broke, easy to wither due to water loss, and meanwhile, the induction quality of seedlings and roots is good, the surviving rate of transplant of the cassava tissue culture seedlings is improved, the working efficiency is improved, and the production cost is reduced.

Description

technical field [0001] The invention relates to the field of agricultural propagation methods, in particular to the field of cassava propagation methods, and in particular to a method for rooting cassava tissue culture seedlings. Background technique [0002] Cassava (Manihotesculenta Crantz), also known as cassava, cassava, cassava, is a plant of the genus Manihot in the family Euphorbiaceae. Large food crops, edible, forage and industrial use, have huge market development space in our country. [0003] Due to the complex genetic background of cassava and severe separation of sexual offspring, seed stem propagation is usually used in production, which limits the promotion and production of new cassava varieties to a certain extent. Tissue culture technology can obtain a large number of propagules in a short time, which is of great significance to speed up the breeding of improved varieties and the promotion of new varieties. At the same time, tissue culture technology is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 严华兵曾文丹谢向誉陆柳英周慧文赖大欣
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI