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Construction method for compound glossy privet fruit for mouse model infected with pig pasteurella multocida

A Pasteurella and mouse model technology, applied in the field of pharmacological experiments, can solve the problems of multiple serotypes and poor vaccine prevention effect

Inactive Publication Date: 2014-10-15
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Pasteurellosis multocida is one of the common diseases in pig farms. At present, vaccines are mainly used to prevent the disease, but the bacteria are widely distributed and have many serotypes, resulting in poor vaccine prevention effect

Method used

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  • Construction method for compound glossy privet fruit for mouse model infected with pig pasteurella multocida
  • Construction method for compound glossy privet fruit for mouse model infected with pig pasteurella multocida
  • Construction method for compound glossy privet fruit for mouse model infected with pig pasteurella multocida

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Experimental program
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Effect test

Embodiment 1

[0038] 1. Experimental materials

[0039] 1.1 Test strain: PM-C439 Pasteurella multocida strain.

[0040] 1.2 Experimental animals: 80 3-week-old and 60 8-week-old SPF grade white male mice were fed with sterilized feed and drinking water, the indoor temperature was 28°C, and the relative humidity was (50±10)%. Animals were fed and adapted to the test after 3 days.

[0041] 1.3 Main reagents: Ligustrum lucidum, Epimedium, Atractylodes macrocephala, and Poria cocos, India ink, lysozyme detection kit (nephelometric method), chocolate agarose medium

[0042] TSB liquid medium: add calf serum to 3% TSB solution to prepare a liquid medium containing 5% CS (V / V) and 3% TSB (M / V).

[0043] 1.4 Main Instruments

[0044] Electric constant temperature water bath, Japan Shimadzu UV spectrophotometer, desktop centrifuge, German automatic high pressure sterilizer, microplate reader, biochemical incubator, ultra-clean workbench

[0045] figure 1 It shows the construction method proces...

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Abstract

The invention discloses a construction method for compound glossy privet fruit for a mouse model infected with pig pasteurella multocida. The method comprises the following steps: preparing the compound glossy privet fruit; grouping and treating the experiment mice for measuring the median lethal dose of pig pasteurella multocida; after feeding, conducting the carbon granule clearance test on the mice, and measuring the activity of lysozyme of mice; measuring the median lethal dose of the pig pasteurella multocida on mice, and measuring the protective effect on the mice infected with pig pasteurella multocida; analyzing the data. According to the method, that the compound glossy privet fruit is used as a feed additive and the activity of lysozyme is improved obviously by increasing the immune organ index and the activity of the mononuclear macrophage is verified, thereby reaching the good protective effect on the mice infected with pig pasteurella multocida.

Description

technical field [0001] The invention belongs to the field of pharmacological experiments, in particular to a method for constructing a mouse model of Pasteurella multocida infected by compound Ligustrum lucidum. Background technique [0002] China is the most important pig farming and pork consumption country in the world, and has a long tradition of pig farming. Pasteurellosis multocida is one of the common diseases in pig farms. At present, vaccines are mainly used to prevent the disease, but the bacteria are widely distributed and have many serotypes, resulting in poor vaccine prevention effect. Pharmacological studies of modern Chinese medicines show that "nourishing" Chinese medicines have a certain immune-enhancing effect. According to the principles of Chinese medicine compatibility and prescription theory, the present invention uses Chinese medicines related to performance, screens and optimizes empirical formulas to obtain Ligustrum lucidum and Epimedium. Huo, Atra...

Claims

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Application Information

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IPC IPC(8): A61K36/638A61K49/00A61P31/04A61P37/04
Inventor 朱丽娜陈仕贵雷凌华陈扬张子烨
Owner HUNAN AGRICULTURAL UNIV
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