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A high-performance liquid chromatograph capable of controlling the flow rate

A high-performance liquid chromatography and control system technology, applied in instruments, scientific instruments, measuring devices, etc., to achieve the effects of improving accuracy, reducing system pressure pulsation, and improving measurement accuracy

Active Publication Date: 2018-04-24
苏州理瞳精密测量系统有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] As mentioned above, the prior art lacks a high-performance liquid chromatograph capable of rapidly responding to changes in system pressure and accurately controlling the flow rate of the mobile phase.

Method used

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  • A high-performance liquid chromatograph capable of controlling the flow rate
  • A high-performance liquid chromatograph capable of controlling the flow rate
  • A high-performance liquid chromatograph capable of controlling the flow rate

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Embodiment Construction

[0036] A preferred embodiment of the present invention will be introduced below in conjunction with the accompanying drawings.

[0037] refer to figure 1 , the high performance liquid chromatograph 1 in the present embodiment comprises solution organizer 101, infusion pump 102, injector 103, chromatographic column 104, detector 105, information processing system 106 and control system 107 etc., wherein solution organizer 101 After the solution in the sample is degassed, it is injected into the system of the liquid chromatograph 1 by the infusion pump 102 as the mobile phase, the sample solution is injected into the mobile phase through the injector 103, and is loaded into the chromatographic column 104 (stationary phase) by the mobile phase ), because the components in the sample solution and the chromatographic column have different polarities, when the sample solution runs relatively in the chromatographic column 104, after repeated adsorption-desorption distribution process...

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Abstract

The invention provides a high-performance liquid chromatograph capable of controlling the flow rate, comprising a double-pump plunger pump in series driven by a cam, a control system, and the series plunger pump including a main pump chamber and a main pump head plunger , an auxiliary pump chamber, an auxiliary pump head plunger, and an outlet check valve for connecting the main pump chamber and the auxiliary pump chamber, the control system is used to control the movement of the main pump head plunger and the auxiliary pump head plunger, The control system is also used to perform the following steps cyclically: obtain a calculated value of the opening point of the outlet check valve according to the system pressure and the configuration parameters of the liquid chromatograph; then obtain a calibration value of the opening point of the outlet check valve according to the system pressure ; Add the calculated value to the calibration value to obtain the actual value of the opening point of the outlet check valve; obtain a compression volume according to the actual value; and control the rotation speed of the cam according to the compression volume. The liquid chromatograph of the invention ensures the mixing ratio of the mobile phase and improves the accuracy of the measurement results.

Description

technical field [0001] The invention relates to the field of liquid chromatography. Background technique [0002] High-performance liquid chromatography is an important branch of chromatography. It uses liquid as the mobile phase and uses a high-pressure infusion system to connect a single solvent with different polarities or a mixed solvent with different proportions, buffer solution, etc. to the sample pump to be tested. The components of the sample to be tested are separated in the column and then enter the detector for detection, thereby realizing the qualitative and quantitative analysis of the sample. This method has become an important separation and analysis technique in the fields of chemistry, medicine, industry, agronomy, commodity inspection and legal inspection. High performance liquid chromatography (HPLC) is the instrument used for this analytical method. [0003] Such as figure 1 As shown, the high performance liquid chromatograph 1 is usually composed of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/32
Inventor 张琰王悦王铁军李维森
Owner 苏州理瞳精密测量系统有限公司
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