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Multi-locus molecular marker-assisted selection method combined with the whole process of conventional wheat breeding

A molecular marker-assisted and molecular marker technology, which is applied in the fields of botany equipment and methods, biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as unsatisfactory results and lack of multi-site molecular marker-assisted selection methods , to achieve the effects of improving breeding efficiency, improving selection accuracy, and improving efficiency

Active Publication Date: 2016-04-13
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the wheat genome is particularly large (1.8×10 10 bp), many repetitive sequences and most of the important traits are quantitative traits controlled by polygenes. The effect of random assisted selection in cross breeding with only 1-2 molecular markers of a certain trait is not ideal. Therefore, the molecular marker assisted selection of wheat The selection scheme still needs to be improved technically, and its high efficiency and large-scale problems need to be solved urgently. Molecular marker-assisted breeding must be organically combined with the whole process of conventional breeding in order to truly realize and play the role of molecular marker-assisted breeding and breed breakthroughs. new wheat variety
[0005] However, there is no multi-locus molecular marker-assisted selection method combined with the whole process of conventional wheat breeding.

Method used

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  • Multi-locus molecular marker-assisted selection method combined with the whole process of conventional wheat breeding
  • Multi-locus molecular marker-assisted selection method combined with the whole process of conventional wheat breeding
  • Multi-locus molecular marker-assisted selection method combined with the whole process of conventional wheat breeding

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Experimental program
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Effect test

Embodiment 1

[0044] Two large-grain gene markers TaGW2-6A and QGW6A-232 were used for multi-locus molecular marker-assisted selection of grain weight, including the following steps:

[0045] Selection of molecular breeding elements and formulation of F1 combinations

[0046] Selection of molecular breeding elements: From February to April 2008, 250 materials created by the "973" project team were identified by using the molecular markers (Hap-6A-P1 / P2) and QGW6A-232 of the grain weight gene TaGW2-6A, Wherein the upstream primer of the molecular marker Hap-6A-P1, the nucleotide sequence is as shown in SEQIDNO.1, the downstream primer of the molecular marker Hap-6A-P1, the nucleotide sequence is as shown in SEQIDNO.2; the molecular marker Hap- The upstream primer of 6A-P2, the nucleotide sequence is shown in SEQIDNO.3, the downstream primer of molecular marker Hap-6A-P2, the nucleotide sequence is shown in SEQIDNO.4; The upstream primer of molecular marker QGW6A-232, nuclear The nucleotide ...

Embodiment 2

[0090] In conventional breeding, using molecular markers of powdery mildew resistance and stripe rust resistance genes to carry out molecular marker-assisted selection of multi-site aggregation of disease resistance genes, and to breed high-yield and multi-resistance wheat Shannong 20, including the following steps:

[0091] 1, contain the screening of the parents of anti-powdery mildew and anti-stripe rust gene: Utilize 15 molecular markers of the anti-powdery mildew gene and 12 molecular markers of the anti-stripe rust gene that have been developed and use effect at present (molecular marker sequence sees Table 5), 100 wheat materials were identified and screened, combined with the surveyed agronomic traits, 6 powdery mildew resistance genes (Pm12, Pm16, Pm24, Pm30, Pm31 and Pm35) and 4 stripe rust resistance genes (Yr9 , Yr24, Yr26 and YrTp1), the parent PH82-2-2 and yield-related traits meet the breeding objectives and contain 6 powdery mildew resistance genes (Pm12, Pm24, ...

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Abstract

The invention relates to a multi-site molecular marker assistant selection method capable of combining with a conventional breeding whole process of wheat. The invention discloses a concrete enforcement method for performing the molecular marker assistant selection technology in the conventional breeding whole process for the first time, the method uses the prerequisite that a molecular marker assistant breeding selection colony is created through a molecule breeding element, the whole breeding process selects genotype through molecular marker, and the method overcomes the disadvantage that conventional breeding selects the genotype according to phenotype.

Description

technical field [0001] The invention relates to a multi-site molecular marker-assisted selection method combined with the whole process of conventional wheat breeding, and belongs to the technical field of molecular marker applications. Background technique [0002] Breeding of new crop varieties is a scientific innovation that has been carried out along with the development of human civilization. From the random collection activities that started about 10,000 years ago to the large-scale production of traditional agriculture, generations of ancestors have left us a rich variety of farm varieties, which are owned by the germplasm banks of various countries A high proportion of breeding materials is also a precious resource for us to create new varieties. [0003] The pea hybridization started by Mendel around 1886 is a symbolic event of modern breeding. In the past 130 years since then, breeders have bred a large number of new crop varieties through sexual hybridization bet...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A01H1/02
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 田纪春邓志英
Owner SHANDONG AGRICULTURAL UNIVERSITY
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