Breeding method of three-line male sterile line with high stress resistance and long panicle neck
A technology of three-line sterile line and long panicle neck, which is applied in the fields of botanical equipment and methods, application, plant gene improvement, etc. It can solve the problems of poor stress resistance and achieve the effect of increasing yield and good stress resistance
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Embodiment 1
[0019] 1) The drought-resistant variety Luhan 1 (A), the cold-resistant variety Dongye No. 1 (B), the saline-alkali-tolerant variety Dongdao No. 4 (C) were selected as resistant parents, and the three-line maintainer line II-32eB1 with long panicle neck was selected as the reincarnation Parent (D), cross A / B and D / C respectively, get cross F 1 Substituted by F 1-AB and F 1-CD express;
[0020] 2) Plant F 1-AB and F 1-CD , in the flowering stage for hybrid F 1-CD / F 1-AB , have to resubmit F 1 (F 1-ABCD ), planting F 1-ABCD , self-inbred to get F 2-ABCD , planting F 2-ABCD , the population is 1200-1500 plants, and the drought resistance, cold resistance, and salt-alkali tolerance tests are carried out at the seedling stage to remove the seedlings that are not drought-resistant, cold-resistant, and salt-alkali-resistant, and list the seedlings with high drought resistance, high cold resistance, and high salt-alkali tolerance. mark, and select the rice plants listed on...
Embodiment 2
[0029] 1) The drought-resistant variety Luhan 1 (A), the cold-resistant variety Dongye No. 1 (B), and the saline-alkali-tolerant variety Dongdao No. 4 (C) were selected as resistant parents, and the three-line maintainer line Xieqingzao eB1 with long panicle neck was The recurrent parent (D) crosses A / B and D / C respectively to obtain cross F 1 Substituted by F 1-AB and F 1-CD express;
[0030] 2) Plant F 1-AB and F 1-CD , in the flowering stage for hybrid F 1-CD / F 1-AB , have to resubmit F 1 (F 1-ABCD ), planting F 1-ABCD , self-inbred to get F 2-ABCD , planting F 2-ABCD , the population is 1200-1500 plants, and the drought resistance, cold resistance, and salt-alkali tolerance tests are carried out at the seedling stage to remove the seedlings that are not drought-resistant, cold-resistant, and salt-alkali-resistant, and list the seedlings with high drought resistance, high cold resistance, and high salt-alkali tolerance. mark, and select the rice plants listed on...
Embodiment 3
[0039] 1) The drought-resistant variety Luhan 1 (A), the cold-resistant variety Dongye No. 1 (B), the saline-alkali-tolerant variety Dongdao No. 4 (C) were selected as the resistant parents, and the three-line maintainer Jin 23eB with long panicle neck was selected as the recurrent parent (D), make crosses A / B and D / C respectively, and get cross F 1 Substituted by F 1-AB and F 1-CD express;
[0040] 2) Plant F 1-AB and F 1-CD , in the flowering stage for hybrid F 1-CD / F 1-AB , have to resubmit F 1 (F 1-ABCD ), planting F 1-ABCD , self-inbred to get F 2-ABCD , planting F 2-ABCD , the population is 1200-1500 plants, and the drought resistance, cold resistance, and salt-alkali tolerance tests are carried out at the seedling stage to remove the seedlings that are not drought-resistant, cold-resistant, and salt-alkali-resistant, and list the seedlings with high drought resistance, high cold resistance, and high salt-alkali tolerance. mark, and select the rice plants lis...
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