Predatory mite toxicity testing method

A measurement method and technology of predatory mite, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high escape rate of predatory mite, difficult to guarantee the accuracy of the results, and strong self-activity, so as to avoid suffocation and death, and the results are accurate Reliable, easy-to-produce results

Active Publication Date: 2014-11-26
CITRUS RES INST SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to their small size, strong mobility and lack of bristles on the back, predatory mites cannot learn from traditional methods for determining the toxicity of spider mites such as the leaf residue method.
At present, the method for assessing the toxicity of predatory mite is mainly the leaf residue method, which is adopted by IOBC (The International Organization for Biological Control, International Organization for Biological Control), and also by EPPO (European and Mediterranean Plant Protection Organization, Europe and Mediterranean Plant Protection Organization) is listed as the official evaluation method, but the escape rate of predatory mite is very high, and the accuracy of the obtained results is difficult to guarantee

Method used

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  • Predatory mite toxicity testing method
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  • Predatory mite toxicity testing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Toxicity Determination of Chlorpyrifos and Lambda-Cyhalothrin to Neoseiusius pastereni

[0026] 1. Materials

[0027] 1.1 Source of tested mite

[0028] The object of the experiment was Neoseius mite, which was collected from the lemon leaves around the orchard of the Citrus Research Institute of the Chinese Academy of Agricultural Sciences.

[0029] 1.2 Chemical reagents: see Table 1

[0030] Table 1 Chemical reagents to be tested

[0031]

[0032] 2 methods

[0033] 2.1 Preparation of reagents with different concentration gradients

[0034] According to the instructions of the chemical reagents, the field concentration of predatory mites was diluted to prepare different concentration gradients. The different concentration gradients of chlorpyrifos and lambda-cyhalothrin set in this experiment are shown in Table 2. Each reagent set 8 different concentration gradients. concentration gradient.

[0035] 2.2 Toxicity assay operation method

[0036] For ...

Embodiment 2

[0054] Example 2 Comparison of the escape rate of Leaf Residue Method and the method of the present invention for Neoseiius pasteurii

[0055] The escape rate of N. pasteurii was compared between the traditional leaf residue method and the method of the present invention. Each method was used to detect 40 Neoseiusius mite, and each method was replicated three times. After 24 hours, the mites on the leaves and in the device of the present invention were counted to calculate the escape rate.

[0056] As shown in Table 3, it can be seen from the significance analysis results that the escape rate of Neoseiius pasteurii using the method of the present invention is significantly lower than the escape rate of the leaf residue method, indicating that the method of the present invention is used to detect the drug resistance of predatory mite. More accurate and reliable.

[0057] Table 3 Leaf residue method compares with the escape rate of the predatory mite virulence assay device of ...

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Abstract

The invention discloses a predatory mite toxicity testing method which comprises the following steps: (1) keeping predatory mites at minus 20 DEG C for 5min so that the predatory mites are under a cold paralytic state, picking the predatory mites on a nylon screen above 300 meshes through a fine writing brush, fully soaking the nylon screen attached with the predatory mites in an agent to be tested and keeping for 5-10s; and then, lifting the nylon screen, placing on a piece of dry filter paper and absorbing residual solution; (2) opening an upper cover of a predatory mite toxicity testing device, putting the nylon screen with the predatory mites in the step 1 onto a mite supporting screen in the predatory mite toxicity testing device, covering the testing device, placing the device on a tray that contains water, breeding the tray in an artificial incubator, observing and recording death rate of the predatory mites after a period and calculating a toxicity testing result. The predatory mite toxicity testing method disclosed by the invention is simple and convenient to operate and is accurate and reliable in result.

Description

technical field [0001] The invention relates to a method for measuring the pesticide resistance of agricultural beneficial insects, in particular to a method for measuring the toxicity of predatory mite. Background technique [0002] Predatory mite is one of the good natural enemies of biological control. It can prey on various pests (mite) such as spider mites, greenhouse whitefly and thrips. It has the advantages of safety and pollution-free. It has been widely used in fruit trees, tea and vegetables abroad. It is also a natural enemy product that is mainly promoted in the green prevention and control of crop pests (mite) in my country. However, chemical control is still an indispensable pest control measure in production. The application of chemical pesticides inevitably leads to the contradiction between chemical control and biological control, especially the application of broad-spectrum insecticides such as organophosphorus and pyrethroids is extremely toxic to predato...

Claims

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Application Information

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IPC IPC(8): G01N33/00
Inventor 丛林冉春陈飞刘浩强李鸿筠田慧霞于士将杨涓丁莉莉
Owner CITRUS RES INST SOUTHWEST UNIV
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