Evernia furfuracea fermented extract and preparation method and application thereof

A technology of extract and tree moss, applied in the field of tobacco additives, to achieve the effects of easy preservation, easy activation and fermentation, strong standardization and controllability

Active Publication Date: 2014-12-03
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, tree moss extract and absolute oil have been used by many tobacco companies to give cigarette products a unique aroma style, but tree moss is used as a substrate and aroma-producing yeast is used for fermentation to prepare tobacco flavor additives. Research not yet reported

Method used

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  • Evernia furfuracea fermented extract and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Media and culture conditions

[0029] The Saccharomyces longans CGMCC 6645 used in the experiment was inoculated in 50 mL of YPD liquid medium, and cultured at 28°C and 160 r / min for 24 hours to obtain activated liquid strains.

[0030] 2. Preparation of Moss Samples

[0031] After the tree moss is fully dried, it is pulverized and passed through a 40-mesh sieve. Weigh 20 g of tree moss powder, add 400 mL of distilled water (20 volume times), sterilize, and set aside.

[0032] 3. Aroma-producing yeast fermentation treatment of tree moss

[0033] Inoculate 8 mL of the activated Saccharomyces longiosa liquid prepared in 1 into the tree moss sample prepared in 2 (2% inoculum), and culture with shaking at 28°C and 160 r / min for 24 hours for the next experiment.

[0034] 4. Extraction of spice additives

[0035] After filtering the above fermented liquid, centrifuge at 8000r / min for 10 minutes, take the supernatant, concentrate it under reduced pressure at 50°C until ...

Embodiment 2

[0048] Repeat Example 1, with the following differences:

[0049] The culture conditions for the activation of the strain CGMCC 6645 are 28°C, 160r / min shaking culture, and the culture time is 24 hours; after the tree moss is crushed, add 22 times of distilled water to sterilize; the activated bacteria solution is inoculated on the surface of the tree moss material The inoculum amount is 2.5%; the fermentation and cultivation time after inoculation is 24 hours; the fermentation liquid supernatant is concentrated under reduced pressure at a temperature of 55° C.; the prepared spice additive is evenly sprayed on the shredded tobacco, and the addition amount is 12 g / box.

[0050] The results of flavoring and smoking test show that the flavor additive can harmonize with the aroma of cigarettes, improve the quality of aroma, increase the amount of aroma, improve the aftertaste, cover up the miscellaneous smell, and have a fresh and special aroma.

Embodiment 3

[0052] Repeat Example 1, with the following differences:

[0053] The culture conditions for the activation of the strain CGMCC 6645 are 30°C, 180r / min shaking culture, and the culture time is 12 hours; after the tree moss is crushed, add 15 times of distilled water to sterilize; the activated bacteria solution is inoculated on the surface of the tree moss material The inoculum amount is 1.5%; the fermentation and cultivation time after inoculation is 12 hours; the supernatant of the fermentation broth is concentrated under reduced pressure at a temperature of 45° C.; the prepared spice additive is evenly sprayed on the shredded tobacco, and the addition amount is 8 g / box.

[0054] The results of flavoring and smoking test show that the flavoring additive can harmonize with the aroma of cigarettes, improve the aroma quality, increase the amount of aroma, improve the aftertaste, cover up the miscellaneous smell, and have a fresh and special aroma.

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Abstract

The invention discloses an Evernia furfuracea fermented extract and a preparation method and application thereof. The Evernia furfuracea fermented extract is prepared from Evernia furfuracea, which serves as a raw material, through fermenting with Lodderomyces elongisporus and extracting aroma components from a fermented solution. The Lodderomyces elongisporus is collected in the China General Microbiological Culture Collection Center (CGMCC); the collection number is CGMCC No. 6645; the collection date is October 08, 2012. The product contains a variety of aroma substances, such as phenethyl alcohol, orcinol monomethyl ether, Alan phenol, beta-orcinol methyl ester, methyl haematommate, ethyl haematommate, ethyl everninate, methyl divaicate and the like. Shown by results of flavored smoking assessment tests, the product can be harmonized with cigarette flavors, can obviously promote the cigarette flavors, make smoke gas delicate and mellow, lower irritation, conceal impurity gases and improve the smoking flavor of cigarettes, and can generate a fresh and special aroma.

Description

technical field [0001] The invention belongs to the technical field of tobacco additives, in particular to a flavor for tobacco obtained by extracting aroma components from moss fermented products. At the same time, the present invention also relates to the preparation method of the tobacco spice and its specific application in tobacco processing. Background technique [0002] Cigarette aroma is the core content of cigarette quality. Whether it is international or domestic, reducing the amount of tar has become an important issue in cigarette production, and with the reduction of the amount of tar, the taste of cigarettes becomes lighter and the aroma is weakened, which is difficult to be accepted by consumers. While reducing cigarette tar, it is an effective compensation method to increase and improve smoke concentration and flavor by adding spice additives. [0003] Natural flavors and fragrances are high-value fine chemical products, but the original natural flavors ext...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A24B3/12C12R1/645
Inventor 陈微王明锋段焰青何雪峰吴俊刘娟曾熠程张翼鹏
Owner CHINA TOBACCO YUNNAN IND
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