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Quick propagation method of panax japonicus C.A . Mey. var.

A fast and efficient technology of ginseng, which is applied in the field of plants, can solve the problems of limitations in the development and utilization of ginseng, and achieve the effects of high reproduction rate, low energy consumption and simple operation

Inactive Publication Date: 2014-12-10
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, wild picking is mainly used, and the propagation is mainly natural reproduction, which has brought limitations to the development and utilization of Panax ginseng

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the shoots of the underground part of Panax ginseng, wash them in clean water, soak them in bleach for 3 minutes, rinse them with running water for 45 minutes, treat them with 75% alcohol on a clean bench for 30 seconds, treat them with mercuric chloride for 10 minutes, and rinse them with sterile water 5-7 times , sterilized Panax ginseng buds were inserted into WPM+6-BA1.0mg / L+2iP0.5mg / L+NAA0.1mg / L+sodium thiosulfate 100mg / L culture medium, with 30g of sucrose added / L, agar 6-7g / L, PH5.8-5.9, light 1500lx, temperature 22°C, the axillary buds of Ginseng angustifolia grown out were put into the proliferation medium WPM+TDZ0.1mg / L+NAA0.1mg / L+2.0mg / LGA 3 Add 30g / L sucrose, 6-7g / L agar, PH5.8-5.9, light 2000lx, put the clustered shoots cultured by proliferation into rooting medium WPM+NAA0.4mg / L, add 25g / L sucrose, agar 5- 6g / L, PH5.8-5.9, light 3000lx, add 5mg / L phenylalanine to the cultured Panax ginseng test tube seedlings, regulate its growth and the content of...

Embodiment 2

[0011] Take the shoots of the underground part of Panax ginseng, wash them in clean water, soak them in bleach for 3 minutes, rinse them with running water for 45 minutes, treat them with 75% alcohol on a clean bench for 30 seconds, treat them with mercuric chloride for 10 minutes, and rinse them with sterile water 5-7 times , sterilized ginseng buds were inserted into WPM+6-BA2.0mg / L+2iP1.0mg / L+NAA0.1mg / L+sodium thiosulfate 100 mg / L culture medium, supplemented with sucrose 30g / L, agar 6-7g / L, PH5.8-5.9, light 1500lx, temperature 22°C, put the axillary buds of Panax japonicus angustifolia into the proliferation medium WPM+TDZ0.1mg / L+NAA0.1mg / L+2.0mg / LGA 3 Add 30g / L sucrose, 6-7g / L agar, PH5.8-5.9, light 2000lx, put the clustered shoots cultured by proliferation into rooting medium WPM+NAA0.4mg / L, add 25g / L sucrose, agar 5- 6g / L, PH5.8-5.9, light 3000lx, add 8 mg / L phenylalanine to the cultured Panax ginseng test tube seedlings to regulate its growth and the content of secon...

Embodiment 3

[0013] Take the shoots of the underground part of Panax ginseng, wash them in clean water, soak them in bleach for 3 minutes, rinse them with running water for 45 minutes, treat them with 75% alcohol on a clean bench for 30 seconds, treat them with mercuric chloride for 10 minutes, and rinse them with sterile water 5-7 times , the sterilized Ginseng buds were inserted into WPM+6-BA1.5mg / L+2iP0.5mg / L+NAA0.1mg / L+sodium thiosulfate 100mg / L culture medium, with 30g of sucrose added / L, agar 6-7g / L, PH5.8-5.9, light 1500lx, temperature 22°C, the axillary buds of Ginseng angustifolia grown out were put into the proliferation medium WPM+TDZ0.1mg / L+NAA0.1mg / L+2.0mg / LGA 3 Add 30g / L sucrose, 6-7g / L agar, PH5.8-5.9, light 2000lx, put the clustered shoots cultured by proliferation into rooting medium WPM+NAA0.4mg / L, add 25g / L sucrose, agar 5- 6g / L, PH5.8-5.9, light 3000lx, add 5mg / L phenylalanine to the cultivated test-tube seedlings of Panax ginseng, regulate its growth and the content...

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Abstract

The invention relates to a quick propagation method of panax japonicus C.A . Mey. var., which comprises the steps of explant disinfection, axillary bud inducing, cluster bud propagation, rootage inducing and regulation and control of phenylalanine. The tissue culture method of the panax japonicus C.A . Mey. var. is easy and simple to operate, achieves high growth coefficient, propagation rate, growth rate and secondary metabolite content, can obtain many seedlings of the panax japonicus C.A . Mey. var. in a short term and contributes to development and utilization of panax japonicus C.A . Mey. var. resources.

Description

technical field [0001] The invention relates to the cultivation of bamboo leaf ginseng under tissue culture conditions, and belongs to the technical field of plants. Background technique [0002] Panax ginseng, perennial herb, Araliaceae, Panax japonicusC.A Mey.var., also known as bamboo root seven, wild notoginseng, chicken head seven, distributed in Sichuan, Guizhou, Yunnan, Tibet and other places. Born in the mountain bushes at an altitude of 2000-3000m. Dispersing blood stasis to stop bleeding; promoting blood circulation to relieve pain; detoxifying and reducing swelling. Indications: hemoptysis, hematemesis, epistaxis, hematuria, hematochezia, metrorrhagia, postpartum hemorrhage, abdominal pain due to blood stasis, amenorrhea, bruises and swelling, traumatic bleeding, sores and swollen poison, snakebite. At present, it is mainly picked in the wild, and the propagation is mainly natural propagation, which has brought limitations to the development and utilization of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH