Novel IgM-IgG polymer and polymerization method thereof
A polymerization method and polymer technology, applied in the direction of hybrid immunoglobulin, etc., can solve the problems of antibody non-specific binding reaction, false positive, false negative, interference, etc.
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Embodiment 1
[0057] Example 1: A novel IgM-IgG polymer and its polymerization method
[0058] Consists of the following steps in turn:
[0059] The first part, IgM and S-acetylthioacetate succinimidyl ester【 N -succinimidyl S-acetylthioacetate (SATA)] (purchased from Pierce, USA)
[0060] In the first step, dissolve IgM immunoglobulin in PBS mixed buffer to obtain IgM immunoglobulin buffer with a concentration of 1mg / ml, then put it into a dialysis bag, and mix it in the PBS at 2-8°C Dialysis in the buffer solution for 8-15 hours; wherein, the PBS mixed buffer solution is a mixed solution of 10mmol / L and pH 7.4 phosphate buffer solution and 0.15mol / L sodium chloride;
[0061] In the second step, remove the dialyzed IgM immunoglobulin buffer in the first step, and then slowly add freshly prepared S-acetylthioacetic acid at a concentration of 10-50 mmol / L to the dialyzed IgM immunoglobulin buffer Dimethyl sulfoxide solution of succinimide ester, shake and mix, and react at 15~35°C for 25~...
Embodiment 2
[0110] Example 2: A novel IgM-IgG polymer and its polymerization method
[0111] Consists of the following steps in turn:
[0112] The first part, the cross-linking of IgG and S-acetylthioacetate succinimidyl ester (purchased from Pierce, USA)
[0113]The first step is to dissolve IgG immunoglobulin in PBS mixed buffer to obtain IgG immunoglobulin buffer with a concentration of 1 mg / ml, then put it into a dialysis bag, and mix it in the PBS at 2-8°C Dialysis in the buffer solution for 8-15 hours; wherein, the PBS mixed buffer solution is a mixed solution of 10mmol / L and pH 7.4 phosphate buffer solution and 0.15mol / L sodium chloride;
[0114] In the second step, remove the dialyzed IgG immunoglobulin buffer in the first step, and then slowly add freshly prepared S-acetylthioacetic acid at a concentration of 10-50 mmol / L to the dialyzed IgG immunoglobulin buffer Dimethyl sulfoxide solution of succinimide ester, shake and mix, and react at 15~35°C for 25~35 minutes to obtain a ...
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