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A method for producing isomaltooligosaccharides from high-concentration starch

A high-concentration isomaltooligosaccharide technology, which is applied in the field of producing high-purity isomaltooligosaccharide with high conversion rate, can solve the problems of low conversion rate and low yield of isomaltooligosaccharide, so as to increase yield and improve enzyme reaction Speed ​​and the effect of reducing production cost

Inactive Publication Date: 2017-05-17
吉林省轻工业设计研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention provides a method for producing isomaltooligosaccharides from high-concentration starch to solve the problem of low yield of isomaltooligosaccharides due to the low conversion rate of α-glucosidase at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) High-concentration starch liquefaction

[0025] In the mixing tank, use 40°C water and first-grade corn starch to prepare starch milk with a concentration of 50%. If the water temperature is too high, the starch will easily gelatinize, and if the water temperature is too low, it will not be easy to mix the starch thoroughly. Therefore, it is necessary to adjust the slurry with warm water, and adjust the pH value to 5.6 with dilute hydrochloric acid (chemically pure) and sodium hydroxide (chemically pure) (this is the optimum pH value of Novozymes’ high-temperature-resistant amylase). ) Add 0.4 liters of high-temperature-resistant α-amylase at a ratio of 0.4 liters of high-temperature-resistant α-amylase, then pump the starch milk from the mixing tank to the heat preservation liquefaction tank, heat it in the jacket with steam, and stir it to the temperature while raising the temperature Stop stirring when it reaches 80°C, keep it warm for 30 minutes, then add high-t...

Embodiment 2

[0033] (1) High-concentration starch liquefaction

[0034] Prepare starch milk with a concentration of 60% in the mixing tank with water at 50°C and first-grade corn starch, and adjust the pH value to 5.6 with dilute hydrochloric acid (chemically pure) and sodium hydroxide (chemically pure) (this is Novozymes’ resistance The optimum pH value of high-temperature amylase), add high-temperature-resistant α-amylase at a rate of 0.5 liters of high-temperature-resistant α-amylase per ton of starch (dry basis), and then pump the starch milk from the mixing tank to heat preservation liquefaction In the tank, use steam to heat in the jacket, stir while raising the temperature until the temperature reaches 85°C, stop stirring, keep warm for 40 minutes, and then add 0.3 liters of high-temperature-resistant α-amylase per ton of starch (dry basis). High-temperature α-amylase is sprayed and liquefied with a jet liquefier. The temperature is controlled at 105°C, and then the flash temperatur...

Embodiment 3

[0042] (1) High-concentration starch liquefaction

[0043] Prepare starch milk with a concentration of 55% in the mixing tank with water at 45°C and first-grade corn starch, and adjust the pH value to 5.6 with dilute hydrochloric acid (chemically pure) and sodium hydroxide (chemically pure) (this is Novozymes’ resistance) Optimum pH value of high temperature amylase), according to the ratio of 0.45 liters of high temperature resistant α amylase per ton of starch (dry basis), add high temperature resistant α amylase, and then pump the starch milk from the mixing tank to heat preservation liquefaction In the tank, use steam to heat in the jacket, stir while raising the temperature until the temperature reaches 83°C, stop stirring, keep warm for 35 minutes, and then add 0.25 liters of high-temperature-resistant α-amylase per ton of starch (dry basis) High-temperature α-amylase, using a jet liquefier for jet liquefaction, the temperature is controlled at 105°C, and then the flash ...

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Abstract

The invention relates to a method for producing isomalto-oligosaccharide from high-concentration starch, which belongs to the field of isomalto-oligosaccharide production. By increasing the initial concentration of starch milk, high-concentration maltodextrin is produced through heat preservation liquefaction and jet liquefaction. The content and concentration of maltose in the medium, and because the addition of pullulanase can reduce the viscosity of high-concentration sugar solution, provide a better substrate environment for α-glucosidase, thereby improving the yield of isomaltooligosaccharides and shortening Glycosylation transition time. The separation and purification technology of isomaltooligosaccharide is not advanced enough, resulting in waste of starch resources. The invention not only improves the purity of the isomaltooligosaccharide by adopting the nanofiltration membrane technology, but also can by-produce high-purity glucose solution and co-produce crystallized glucose, thereby effectively solving the problem of resource utilization.

Description

technical field [0001] The invention relates to the technical field of isomalto-oligosaccharide production, in particular to a production method for producing high-purity isomalt-oligosaccharide with a high conversion rate by using more than 50% high-concentration cornstarch milk as a raw material. Background technique [0002] Isomaltooligosaccharide (IMO) is a kind of starch sugar, its main component is isomaltose (IG) bound by α-1,6-glucosidic bond 2 ), panose (P), isomaltotriose (IG 3 ) and more than four sugars (including four sugars) (G n ) of oligosaccharides. It is a kind of starch syrup (powder) produced by enzymatic method. Isomaltooligosaccharide has mellow and soft sweet taste, low viscosity, heat and acid resistance, non-fermentability and good moisture retention. In terms of physiological functions, isomaltooligosaccharide has health functions such as improving the structure of intestinal flora, resisting dental caries, regulating body metabolism, improving ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14
Inventor 杨志强于涛张春泓李涛
Owner 吉林省轻工业设计研究院
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