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Programmed temperature rise sterilization method of edible fungi culture material

A sterilization method and culture material technology, applied in heating and other directions, can solve the problems of folic acid loss and affecting mycelial growth, etc., and achieve the effects of convenient operation, reduced nutrient loss, and less investment

Inactive Publication Date: 2014-12-24
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Different manufacturers have different specific procedures for autoclaving, and different sterilization methods will cause different degrees of loss of folic acid and nicotinic acid, which are necessary for the vegetative growth of fungi in the compost, and then affect the growth of mycelia. to grow

Method used

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  • Programmed temperature rise sterilization method of edible fungi culture material
  • Programmed temperature rise sterilization method of edible fungi culture material
  • Programmed temperature rise sterilization method of edible fungi culture material

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Embodiment 1: A kind of sterilization method of edible fungus compost

[0016] Adopt the three-stage stepwise program sterilization method, that is, keep the temperature at 92°C for 60 minutes, raise the temperature to 103°C and keep it for 60 minutes, then raise the temperature to 122°C and keep it for 90 minutes, and simmer for 90 minutes. When the pressure of the autoclave drops to zero, the temperature drops to 90 ℃, open the sterilizer, start the air filter, and push out the sterilizer for cooling.

Embodiment 2

[0017] The determination and analysis of the content of folic acid in the compost of different sterilization methods of embodiment 2

[0018] Sterilization method 1: Sterilize at 121°C for 2 hours;

[0019] Sterilization method 2: 60 minutes at 100°C, intermittent vacuum pumping, the pressure in the kettle is pumped to -0.05~-0.06MPa, and it takes 15 minutes to raise the temperature in the kettle to 121°C, keep the pressure at 0.11MPa for 90 minutes, and turn off the steam , the sterilization is over;

[0020] Sterilization method 3: Sterilize at 100°C for 15 hours;

[0021] Sterilization method 4: the sterilization method described in Example 1;

[0022] Take 4.2g of the culture material obtained by the above-mentioned different sterilization methods and add 15mL of 0.1mol / mL NaOH buffer solution to 12 centrifuge tubes (3 centrifuge tubes for each), and put them into a digitally controlled ultrasonic cleaner for 40 minutes of ultrasonication. Take out the filter and measur...

Embodiment 3

[0028] The determination and analysis of the content of nicotinic acid in the compost of different sterilization methods in embodiment 3

[0029] Take 4.2 g of the culture material obtained by different sterilization methods described in Example 1, add 15 mL of distilled water to 12 centrifuge tubes (3 centrifuge tubes for each), and put them into a digitally controlled ultrasonic cleaner for 60 minutes of ultrasonication. Take out the filter and measure the absorbance at different wavelengths with a UV spectrophotometer. The data were brought into the corresponding standard curve, and the content of niacin in the compost was calculated. The results are shown in Table 2.

[0030] Preparation of niacin standard curve: Accurately weigh an appropriate amount of niacin standard product (calculated as anhydrous matter), dissolve it in distilled water, and prepare a niacin stock solution with a concentration of 100 μl / mL. Prepare 5, 10, 15, 25, 30, 40, 60 μl / mL solutions, use disti...

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Abstract

The invention discloses a programmed temperature rise sterilization method of an edible fungi culture material, and belongs to the technical field of edible fungi cultivation. The sterilization method adopts a three-step ladder type, and comprises the following steps: firstly, putting the culture material into a sterilization pot, rising the temperature to 90 to 95 DEG C, keeping the temperature for 60 min, secondly, rising the temperature to 105 to 110 DEG C continuously, keeping the temperature for 60 min, thirdly, rising the temperature to 121 to 125 DEG C continuously, keeping the temperature for 90 min, braising the culture material for 90 min, fourthly, opening the sterilization pot when the air pressure of the sterilization pot is reduced to zero and the temperature is reduced to 85 to 95 DEG C, starting an air filter, and cooling the culture material. The method can reduce the loss of nutrient substances namely folic acid, nicotinic acid, vitamin B and vitamin C required by the edible fungi during the sterilization process, provides more nutrient elements for the thallus growth, and simultaneously guarantees the sterilization effect.

Description

Technical field: [0001] The invention belongs to the technical field of fungi cultivation, and in particular relates to a programmed temperature rise sterilization method and its application in the sterilization of edible fungus compost. Background technique: [0002] There are a large number of microorganisms and insect pests such as fungi, bacteria, actinomycetes, and viruses in the edible fungi compost, and their existence will compete with edible fungi for nutrients and damage edible fungi. Therefore, in the cultivation process of edible fungi, the sterilization of compost is an important link. The sterilization method of edible fungus compost in the prior art is mainly moist heat sterilization, and moist heat sterilization includes three types: high pressure sterilization, atmospheric pressure steam sterilization and intermittent sterilization. [0003] Sterilization in factory cultivation generally adopts steam autoclaving, that is, put the culture material into the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L2/06A61L2/07
Inventor 班立桐杨红澎王玉黄亮
Owner TIANJIN AGRICULTURE COLLEGE
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