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A method for extracting and purifying selenium-containing protein from broken rice chestnuts in decolorized pots and bottles

A pot and bottle crushed rice chestnut and a purification method technology, which is applied in the field of purification and decolorization of pot and bottle crushed rice chestnuts to extract selenoproteins, can solve the problems of limited application range, affecting the biological activity of crushed rice chestnuts containing selenoproteins, etc., and achieve Effect of improving competitiveness and good free radical scavenging activity

Active Publication Date: 2018-02-27
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the water-extracted spray-dried product of crushed rice catkins in pots and bottles is dark brown, and its application range is limited. Therefore, the extract must be decolorized
At present, the main methods of decolorization are activated carbon method and hydrogen peroxide method, but our research has found that these two methods will greatly affect the biological activity of selenoproteins in broken rice chestnuts, such as antioxidant activity. We use our unique decolorization and purification process , obtained selenoproteins containing light yellow selenoproteins and evaluated their protein properties and antioxidant activity. The results showed that the decolorization and purification techniques had no adverse effects on its activity

Method used

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  • A method for extracting and purifying selenium-containing protein from broken rice chestnuts in decolorized pots and bottles
  • A method for extracting and purifying selenium-containing protein from broken rice chestnuts in decolorized pots and bottles
  • A method for extracting and purifying selenium-containing protein from broken rice chestnuts in decolorized pots and bottles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) The preparation of the extract of broken rice chestnuts in pots and bottles:

[0032] Grind and sieve the dried potted rice chestnut, then add water to the potted crushed rice chestnut powder and soak for 20 minutes, the ratio of solid to liquid is 1:20, then start heating, stir and extract at 75°C for 80 minutes, continue to heat up Stir and extract at 85°C for 80 minutes, then filter, and spray-dry the filtrate to obtain a pot of crushed rice chestnut extract powder;

[0033] (2) Decolorization of pot bottle broken rice chestnut extract:

[0034] Dissolve the extract powder of broken rice chestnut in the pot bottle prepared in step (1) in distilled water, and the weight-to-volume ratio of the broken rice chestnut extract powder in the pot bottle to distilled water is 5:200g / ml, and then adjust the pot bottle broken rice chestnut extract The pH value of the solution reaches the isoelectric point of the crushed oriole protein in the pot, and it is left to stand for...

Embodiment 2

[0043] (1) The preparation of the extract of broken rice chestnuts in pots and bottles:

[0044] Crush and sieve the dried potted rice chestnut, then add water to the potted crushed rice chestnut powder and soak for 40 minutes, the ratio of solid to liquid is 1:50, then start heating, stir and extract at 84°C for 40 minutes, continue to heat up at Stir and extract at 95°C for 40 minutes, then filter, and spray-dry the filtrate to obtain a pot of crushed rice chestnut extract powder;

[0045] (2) Decolorization of pot bottle broken rice chestnut extract:

[0046] Dissolve the extract powder of broken rice chestnut in the pot bottle prepared in step (1) in distilled water, and the weight-to-volume ratio of the broken rice chestnut extract powder in the pot bottle to distilled water is 15:200g / ml, and then adjust the pot bottle broken rice chestnut extract The pH value of the solution reaches the isoelectric point of the crushed oriole protein in the pot, and it is left to stand...

Embodiment 3

[0055] (1) The preparation of the extract of broken rice chestnuts in pots and bottles:

[0056] Grind and sieve the dried potted rice chestnut, then add clear water to the potted crushed rice chestnut powder and soak for 30 minutes. Stir and extract at 90°C for 60 minutes, then filter, and spray-dry the filtrate to obtain a pot of crushed orchardrum extract powder;

[0057] (2) Decolorization of pot bottle broken rice chestnut extract:

[0058] Dissolve the extract powder of the broken rice chestnut in the pot bottle prepared in step (1) in distilled water, the weight volume ratio of the broken rice chestnut extract powder in the pot bottle to the distilled water is 10:200g / ml, then adjust the broken rice chestnut extract in the pot bottle The pH value of the solution reaches the isoelectric point of the crushed rice chestnut protein in the pot, and it is left to stand for 12 hours, and then centrifuged at 4°C and 6000 r / min for 10 minutes, the precipitate is collected and v...

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Abstract

The invention discloses an extraction and purification method of decolorized cardamine hupingshanesis selenium-containing proteins, which comprises the following steps: (1) preparation of cardamine hupingshanesis extract; (2) decolorization of cardamine hupingshanesis extract; and (3) graded purification of cardamine hupingshanesis crude proteins. The method can obviously improve the color of the cardamine hupingshanesis extract, is advanced and scientific, and is convenient to operate; the cardamine hupingshanesis crude proteins and purified selenium-containing proteins still have favorable anti-oxidation activity; and the method provides a favorable technical means and use value for application of cardamine hupingshanesis when being used for preparing health-care products with anti-oxidation and antiaging actions, and thus, has huge economic and social benefits.

Description

technical field [0001] The invention relates to the technical field of plant extracts, in particular to a method for extracting and purifying selenoproteins from decolorized pot and bottle crushed catkins. Background technique [0002] Huping broken rice chestnut is a cruciferous plant with strong selenium-enriching ability. The selenium content of the water-extracted and spray-dried product of Huping broken rice chestnut is as high as 1000mg / kg, of which the organic selenium content accounts for about 93.30%. The added components of selenium health care products have the characteristics of high safety and good absorption, and have a large application space. [0003] At present, the water-extracted spray-dried product of crushed rice chestnuts in pots and bottles is dark brown, and its application range is limited. Therefore, the extract must be decolorized. At present, the main methods of decolorization are activated carbon method and hydrogen peroxide method, but our rese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/18C07K1/16C07K1/14
Inventor 徐晓云田秀丽
Owner HUAZHONG AGRI UNIV
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