Primers for detecting breast cancer susceptibility gene SNP (single nucleotide polymorphism), fluorescent probes and applications

Abstract

The invention discloses primers for detecting the breast cancer susceptibility gene SNP (single nucleotide polymorphism), fluorescent probes and applications. The primers and corresponding fluorescent probe groups are used for performing rapid genetic typing on rs1219648, rs13281615, rs2046210, rs3803662, rs8051542, rs3817198 and rs889312 respectively. Detection results obtained by the aid of the primers and the fluorescent probes are accurate and reliable, and costs are lower, so that the primers and the fluorescent probes have the higher practicability and are particularly applicable to large-scale screening of breast cancer high-risk groups in regions in China.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer for detecting breast cancer susceptibility gene SNP, a fluorescent probe and application thereof. Background technique [0002] Breast cancer is one of the most common cancers among women all over the world, and its incidence rate ranks first among cancers among women in China. According to the data released by the Ministry of Health, the incidence of breast cancer in my country is still on the rise. In developed countries such as the United States, the morbidity and mortality of breast cancer are declining, not only because of the improvement of diagnosis and treatment, but also because of the early screening. Common screening for breast cancer includes self-examination, B-ultrasound examination, X-ray examination and so on. These inspection methods are all based on the occurrence of breast cancer. If screening can be performed before breast cancer occurs and high-risk gr...

AI Technical Summary

Problems solved by technology

However, this method is complicated to operate, and when the sample size is large, it is easy to cause cross-contamination of PCR products, and it is prone to insufficient or excessive digestion, resulting in false negative or false positive results, and low reliability.

Although the specificity of the multiplex PCR method has been improved, the principle of this method is still based on the principle of ordinary PCR, and factors such as low primer specificity and low-fidelity Taq enzymes can affect the results

Although the DNA sequencing method is currently the gold standard for genetic diagnosis, the steps are cumbersome, the process is complicated, the reagents are expensive, and cross-contamination between samples is prone to occur, resulting in sequencing failures; in addition, the equipment of the sequencer is beyond that of a general clinical laboratory. Tolerance range

Chip detection is more suitable for high-throughput gene detection, not suitable for detection of several genes

The high-resolution melting curve method in fluorescent quantitative PCR is a fast, simple, economical, and practical typing method, but genotyping depends on the precision of instrument temperature control, and the false positive rate is high.

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