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Kit for detecting amplification of REL gene

A detection kit and gene amplification technology, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of inaccurate results and achieve simple operation, suitable for large-scale promotion and application, The effect of high specificity and sensitivity

Inactive Publication Date: 2014-12-24
天津蒙特立医疗科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the PCR method is mainly used to detect REL gene amplification in the market. Since the detection is a mixture of tumor and normal cells, the result is not accurate enough, and a more accurate detection method is urgently needed in the market.

Method used

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  • Kit for detecting amplification of REL gene
  • Kit for detecting amplification of REL gene
  • Kit for detecting amplification of REL gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Acquisition of probe DNA

[0018] 1) Streak inoculation: Streak inoculate the BAC strain carrying the REL gene and the centriole of chromosome 2 on an agar plate containing antibiotics (chloramphenicol), and culture at 37° C. overnight (about 14 hours).

[0019] 2) Initial culture: Pick a monoclonal strain with good growth status the next day, inoculate it in 2ml of LB liquid medium containing antibiotics (chloramphenicol), put it in a shaker at 37°C, set the shaker speed to about 200rpm, and culture 6-8 hours.

[0020] 3) Overnight culture: transfer each 1ml of bacterial liquid to 200ml culture system, place on a shaker at 37°C with a rotation speed of 200rpm, and culture overnight (about 14-16 hours).

[0021] 4) On the next day, a large amount of DNA was extracted and purified according to the operating instructions of the QIAGEN plasmid mass extraction kit.

[0022] 2. Probe labeling

[0023] 1. Digestion of DNA:

[0024] 1) Digest the target DNA according to...

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Abstract

The invention relates to a kit for detecting the amplification of an REL gene. The kit comprises a group of bi-color FISH probes, wherein one group of bi-color probes (1) are REL gene probes (1) marked to be red and No.2 chromosome centriole probes (2) marked to be green, wherein the REL is a nucleotide sequence shown as SEQ ID NO:1. According to the kit disclosed by the invention, the specificity and the sensitivity are strong; the amplification conditions of the REL genes in lung cancer cells can be detected quickly, accurately and visually; the detection result is accurate and reliable; the kit is suitable for large-scale popularization and use.

Description

technical field [0001] The invention relates to the technical field of disease-related gene detection, in particular to a FISH detection method for lung cancer REL gene amplification, related detection probes and a kit. Background technique [0002] Lung cancer is one of the malignant tumors with the fastest increasing morbidity and mortality and the greatest threat to the health and life of the population. In the past 50 years, many countries have reported that the morbidity and mortality of lung cancer have increased significantly. The incidence and mortality of lung cancer in men rank first among all malignant tumors, and the incidence and mortality of lung cancer occupy the second place in women. The etiology of lung cancer is still not completely clear. A large number of data show that long-term heavy smoking has a very close relationship with the occurrence of lung cancer. [0003] According to the growth, invasion and metastasis speed and scope of lung cancer and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q1/6841C12Q2600/124
Inventor 缪为民
Owner 天津蒙特立医疗科技有限责任公司