Rapid propagation method of temstroemia gymnanthe

A thick-skinned and fast technology, applied in the field of plants, can solve the problems of no research and achieve the effects of less energy consumption, large-scale planting, and short cycle

Inactive Publication Date: 2015-01-07
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Utilizing tissue culture method can effectively improve the reproductive efficiency of A. pachyrhiza, but there is no research at home and abroad at present.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the thick-skinned incense stick bud stem, soak it in bleaching powder for 3 minutes, remove the surface stains with a brush, wash it with running water for 25 minutes, treat it with 75% alcohol on an ultra-clean workbench for 30 seconds, treat it with sodium hypochlorite for 12 minutes, rinse it with sterile water 5-7 times, The sterilized A. saponica buds and stems are inserted into the culture medium with MS +IAA0.2mg / L+IBA0.5mg / l induction medium for bud induction culture, with 30g / L sucrose and 8g / L agar , PH5, light 1000lx, put the induced buds into proliferation medium B 5 +2,4-D0.1mg / L+TDZ0.2mg / L+ABA1mg / L, add sucrose 30g / L, agar 8g / L, PH5, light 1000lx, light period 10h, dark period 14h, proliferate and cultivate clusters Put the buds into the rooting medium White +NAA0.1mg / L, the rooting culture conditions are 20g / L sucrose, 6.5g / L agar, PH5, 1000lx light, 60-70% humidity, and then transplant the test-tube seedlings for hardening. The survival rate of thi...

Embodiment 2

[0011] Take the thick-skinned incense stick bud stem, soak it in bleaching powder for 3 minutes, remove the surface stains with a brush, wash it with running water for 25 minutes, treat it with 75% alcohol on an ultra-clean workbench for 30 seconds, treat it with sodium hypochlorite for 12 minutes, rinse it with sterile water 5-7 times, The sterilized thick-skinned saponin buds and stems are inserted into the medium formula of White+IAA0.2mg / L+IBA0.5mg / l induction medium for bud induction culture, additional sucrose 30g / L, agar 8g / L , PH5, light 1000lx, put the induced buds into proliferation medium B 5 +2,4-D0.2mg / L+TDZ0.5mg / L+ABA1mg / L, add sucrose 30g / L, agar 8g / L, PH5, light 1000lx, light period 10h, dark period 14h, proliferate and cultivate clusters Put the buds into the rooting medium White +NAA0.2mg / L, the rooting culture conditions are 20g / L sucrose, 6.5g / L agar, PH5, 1000lx light, 60-70% humidity, and then transplant the test-tube seedlings for hardening. The surviva...

Embodiment 3

[0013] Take the thick-skinned incense stick bud stem, soak it in bleaching powder for 3 minutes, remove the surface stains with a brush, wash it with running water for 25 minutes, treat it with 75% alcohol on an ultra-clean workbench for 30 seconds, treat it with sodium hypochlorite for 12 minutes, rinse it with sterile water 5-7 times, The sterilized thick-skinned scallop buds and stems are inserted into the medium formula of White +IAA0.2mg / L+IBA0.5mg / l induction medium for bud induction culture, with 30g / L sucrose and 8g / L agar , PH5, light 1000lx, put the induced buds into proliferation medium B 5 +2,4-D0.2mg / L+TDZ0.2mg / L+ABA1mg / L, add sucrose 30g / L, agar 8g / L, PH5, light 1000lx, light period 10h, dark period 14h, proliferate and cultivate clusters Put the buds into the rooting medium White +NAA0.1mg / L, the rooting culture conditions are 20g / L sucrose, 6.5g / L agar, PH5, 1000lx light, 60-70% humidity, and then transplant the test-tube seedlings for hardening. The survival ...

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PUM

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Abstract

The invention relates to a rapid propagation method of temstroemia gymnanthe. The rapid propagation method comprises disinfection of explants, bud induction culture, multiple shoot proliferation, rooting culture and acclimatization and transplanting. A tissue culture method of temstroemia gymnanthe, disclosed by the invention, is simple and convenient to operate, is short in growth cycle, high in survival rate and stable in genetic character, and can obtain a great number of temstroemia gymnanthe seedlings in a short period.

Description

technical field [0001] The invention relates to a rapid propagation method for tissue culture of A. pachyrhiza and belongs to the technical field of plants. Background technique [0002] thick skin fragrance, Ternstroemia gymnanthera (Wight et Arn.) Beddome., Camellia, shrub or small tree, also known as white flower fruit, called pole red, mo red cut, camellia tree, pig blood wood, blood vine. Distributed in Hubei, Hunan, Guizhou, Yunnan, Guangxi, Fujian, Guangdong, Taiwan and other provinces. There are also distributions in Japan, Cambodia, and India. It mostly grows in the evergreen broad-leaved forest or forest edge of acidic yellow soil and yellow brown soil, and is vertically distributed between 700 and 3500m above sea level. It has strong adaptability, shade tolerance, round crown, strong layering of branches and leaves, and thick leaves that turn crimson in winter. It likes a humid environment and grows vigorously under evergreen broad-leaved trees. It is also li...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张金芳杨存
Owner NANJING BIAOKE BIO TECH
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