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A method for detecting mechanical beating of cardiomyocytes based on optical image analysis

A cardiomyocyte and optical image technology, applied in the field of cell optical image detection and analysis, can solve the problems of complex operation, high cost, complex chip processing technology, etc., and achieve the effect of simple operation steps and low cost

Inactive Publication Date: 2016-06-08
ZHEJIANG UNIV
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Problems solved by technology

Therefore, the operation is complicated and there are limitations in drug application
Micromechanical methods, such as silicon micro-cantilever arrays and vertical PDMS micro-column arrays, also have disadvantages such as complex chip processing technology, complex experimental operations, and high costs.

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  • A method for detecting mechanical beating of cardiomyocytes based on optical image analysis
  • A method for detecting mechanical beating of cardiomyocytes based on optical image analysis
  • A method for detecting mechanical beating of cardiomyocytes based on optical image analysis

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Embodiment Construction

[0026] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments, but the present invention is not limited.

[0027] The present invention is based on the cardiac muscle cell mechanical beating detection method of optical image analysis, and this method is realized on the cardiac muscle cell mechanical beating detection system, such as figure 1 As shown, the cardiomyocyte mechanical beating detection system includes: a computer 1, a CCD camera 3, an upright microscope 4 and a 96-well cell culture plate 5; wherein, the computer 1 and the CCD camera 3 are electrically connected by a USB connection line 2; the CCD camera 3 is fixed on the top of the upright microscope 4 by threads; the 96-well cell culture plate 5 is fixed on the stage of the upright microscope 4; the method includes the following steps:

[0028] (1) Cardiomyocyte culture: place the apex of the rat heart in pre-cooled high-glucose medium...

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Abstract

The invention discloses a cardiomyocyte mechanical beating detection method based on optical image analysis. The method adopts image processing such as image acquisition, RGB image conversion to grayscale image, image binarization, and image matrixing through a cardiomyocyte mechanical beating detection system. Analytical technology to detect the change of the beating image during the mechanical beating of myocardial cells, and use the difference value of the beating image to quantify the mechanical beating of cardiomyocytes to realize the detection of the mechanical beating curve of cardiomyocytes; The algorithm calculates the rate and amplitude of cardiomyocyte pulsation, and analyzes the state of cardiomyocyte mechanical pulsation; compared with the existing cardiomyocyte mechanical pulsation detection method, the present invention has the advantages of no label, no loss, low cost, simple operation steps and synchronous and intuitive detection of cell pulsation Image and analysis of cell beating state and other advantages.

Description

technical field [0001] The invention relates to cell optical image detection and analysis technology, in particular to a method for detecting cardiac muscle cell mechanical beating based on optical image analysis. Background technique [0002] In recent years, it has become a hot topic in the field of cardiology to study changes in the contractile function of cardiomyocytes induced by heart disease or drugs. Rhythmic contraction is the basic feature of cardiomyocytes and the source of power for the whole body's blood circulation system. It is closely related to the basic components of cardiomyocytes, such as microtubules, sarcoplasmic reticulum, and actin, and is an important reflection of cardiac function. In addition, drugs can also cause changes in the contractile function of cardiomyocytes. The detection of cardiomyocyte mechanical beating is of great significance in the field of medicine and drug research. At present, laser confocal microscopy calcium imaging technol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02G06T7/00
Inventor 王平苏凯麒胡宁王琴黎洪波邹玲曹端喜邹瞿超
Owner ZHEJIANG UNIV
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